TBX10 (NM_005995) Human Untagged Clone

CAT#: SC303717

TBX10 (untagged)-Human T-box 10 (TBX10)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TBX10
• Synonyms: TBX7; TBX13
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC303717 representing NM_005995.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGCAGCCTTCCTATCTGCTGGCCTCGGCATACTTGCACCCTCAGAGACCTACCCCCTACCTACAACC
  AGCTCTGGCTGGGAGCCCCGGCTGGGGTCACCATTCCCATCAGGCCCTTGCACCAGCTCTACTGGGGCC
  CAAGCTGTGGCCGAGCCCACTGGGCAGGGCCCCAAGAACCCACGTGTGTCCAGAGTGACAGTTCAGCTG
  GAGATGAAGCCTCTGTGGGAGGAATTCAACCAGCTGGGCACTGAGATGATCGTCACCAAGGCAGGCAGG
  AGGATGTTCCCCCCCTTCCAGGTGAAGATCCTGGGCATGGACTCCCTGGCCGACTACGCCCTGCTCATG
  GACTTCATCCCCCTGGACGACAAGAGATACAGGTATGCCTTCCACAGCTCGGCCTGGCTGGTGGCGGGC
  AAGGCAGACCCAGCCACACCTGGCCGCGTGCACTTCCACCCCGACTCGCCAGCCAAGGGTGCCCAGTGG
  ATGCGCCAGATTGTGTCCTTTGACAAGCTCAAGCTGACCAACAACCTGCTGGATGACAATGGCCACATC
  ATTCTCAACTCTATGCACCGCTACCAGCCCCGTTTCCACGTGGTCTTCGTGGACCCACGCAAGGACAGT
  GAGCGCTATGCCCAGGAGAACTTCAAGTCCTTCATCTTCACAGAGACCCAGTTCACAGCAGTGACAGCC
  TATCAGAACCACAGGATCACCCAGCTGAAAATCGCCAGCAACCCTTTTGCCAAAGGCTTTAGAGAGAGT
  GACCTGGACTCCTGGCCTGTGGCCCCACGGCCCCTGCTCAGTGTCCCAGCCCGGAGTCACAGCAGCCTC
  AGTCCCTGTGTGCTGAAGGGTGCCACAGACAGGGAGAAAGACCCCAACAAAGCTTCAGCTTCCACCTCC
  AAGACCCCTGCTTGGCTCCATCATCAGCTGCTGCCCCCACCTGAGGTCCTGCTGGCCCCGGCCACCTAC
  AGGCCTGTCACGTATCAGAGCCTGTACTCTGGAGCCCCGAGCCACCTAGGGATCCCAAGGACCCGACCA
  GCACCATACCCCCTCCCCAACATCCGGGCTGATAGGGATCAAGGAGGCCTGCCTCTCCCAGCTGGGCTG
  GGGCTCCTGTCCCCCACTGTGGTGTGCCTGGGGCCTGGCCAGGACTCCCAGTGA
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_005995
• Insert Size: 1158 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005995.4
• RefSeq Size: 1557 bp
• RefSeq ORF: 1158 bp
• Locus ID: 347853
• UniProt ID: O75333
• Cytogenetics: 11q13.2
• MW: 42.3 kDa
• Gene Summary: This gene encodes a member of the T-box family of transcription factors. These transcription factors share a DNA-binding domain called the T-box, and play a role in several developmental processes including early embryonic cell fate and organogenesis. The encoded protein is a member of the T-box 1 subfamily. Mutations in this gene are thought to be a cause of isolated cleft lip with or without cleft palate. [provided by RefSeq, Nov 2010]