TAF1C (NM_005679) Human Untagged Clone

CAT#: SC303681

TAF1C (untagged)-Human TATA box binding protein (TBP)-associated factor, RNA polymerase I, C, 110kDa (TAF1C), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TAF1C
• Synonyms: MGC:39976; SL1; TAFI95; TAFI110
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC303681 representing NM_005679.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGACTTCCCCAGCTCCCTCCGCCCTGCATTGTTTCTGACCGGCCCCCTTGGTCTGAGCGACGTCCCT
  GACCTCTCTTTCATGTGCAGCTGGCGAGACGCACTGACTCTGCCAGAGGCCCAGCCCCAGAACTCAGAG
  AATGGGGCACTGCATGTGACCAAGGACCTGCTGTGGGAGCCGGCAACCCCTGGGCCTCTCCCCATGCTG
  CCTCCCCTCATCGATCCCTGGGACCCTGGCCTGACTGCCCGGGACCTGCTTTTCCGCGGAGGGTACCGG
  TATCGGAAGCGGCCCCGAGTCGTGCTGGATGTGACTGAGCAGATCAGCCGGTTCCTCTTGGATCATGGA
  GACGTAGCCTTTGCGCCCCTGGGGAAGCTGATGCTGGAGAATTTCAAGCTGGAGGGAGCGGGGAGCCGC
  ACTAAGAAGAAGACAGTGGTCAGTGTGAAGAAGCTGCTCCAGGACCTCGGTGGACACCAGCCCTGGGGG
  TGTCCCTGGGCTTACCTCAGCAACCGACAGCGCCGCTTCTCTATCCTCGGGGGCCCCATCCTGGGCACG
  TCGGTGGCGAGCCACTTGGCAGAGCTGCTGCACGAGGAGCTGGTGCTGCGGTGGGAGCAGCTGCTTCTG
  GATGAGGCCTGCACTGGGGGCGCGCTGGCCTGGGTTCCTGGAAGGACACCCCAGTTCGGGCAGCTGGTC
  TACCCTGCTGGAGGCGCCCAGGACAGGCTGCATTTCCAAGAGGTCGTTCTGACCCCAGGTGACAATCCC
  CAATTCCTTGGGAAACCTGGACGCATCCAGCTCCAGGGACCTGTCCGGCAAGTGGTGACATGCACCGTC
  CAGGGAGAAAGTAAGGCCCTTATATACACTTTCCTCCCTCACTGGCTGACCTGCTACCTGACCCCTGGC
  CCTTTCCATCCCTCCTCAGCTCTGCTGGCCGTCCGCTCTGACTACCACTGTGCCGTGTGGAAGTTTGGT
  AAACAGTGGCAGCCAACCCTTCTGCAGGCAATGCAGGTGGAGAAAGGGGCCACGGGGATCAGCCTCAGC
  CCTCACCTGCCCGGGGAGCTGGCCATCTGCAGCCGCTCGGGAGCCGTCTGCCTGTGGAGCCCTGAGGAT
  GGGCTGCGGCAAATCTACAGGGACCCTGAGACCCTCGTGTTCCGGGACTCCTCTTCGTGGCGTTGGGCA
  GACTTCACTGCGCACCCTCGGGTGCTGACCGTGGGTGACCGCACCGGAGTGAAGATGCTGGACACTCAG
  GGCCCGCCGGGCTGTGGTCTGTTGCTTTTTCGTTTGGGGGCAGAGGCTTCGTGCCAGAAAGGGGAACGT
  GTCCTGCTTACCCAGTACCTGGGGCACTCCAGCCCCAAATGCCTCCCCCCTACTCTTCATCTCGTCTGT
  ACCCAGTTCTCTCTCTACCTAGTGGACGAGCGCCTTCCCCTGGTGCCGATGCTGAAGTGGAACCATGGC
  CTCCCCTCCCCGCTCCTGCTGGCCCGACTGCTGCCTCCGCCCCGGCCCAGCTGCGTGCAGCCCCTGCTC
  CTCGGAGGCCAGGGTGGGCAGCTGCAGCTGCTGCACCTGGCAGGAGAAGGGGCGTCGGTGCCCCGCCTG
  GCAGGCCCCCCCCAGTCTCTTCCTTCCAGGATCGACTCCCTCCCTGCATTTCCTCTGCTGGAGCCTAAG
  ATCCAGTGGCGGCTGCAGGAGCGCCTGAAAGCACCGACCATAGGTCTGGCTGCCGTCGTCCCGCCCTTG
  CCCTCAGCGCCCACACCAGGCCTGGTGCTCTTCCAGCTCTCGGCGGCGGGAGATGTCTTCTACCAGCAG
  CTCCGCCCCCAGGTGGACTCCAGCCTCCGCAGAGATGCTGGGCCTCCTGGCGACACCCAACCTGACTGC
  CATGCCCCCACAGCTTCCTGGACCTCCCAGGACACTGCCGGCTGCAGCCAGTGGCTGAAGGCCCTGCTA
  AAAGTGCCCCTGGCTCCTCCTGTGTGGACAGCACCCACCTTCACCCACCGCCAGATGCTGGGCAGCACA
  GAGCTGCGGAGGGAGGAAGAGGAAGGGCAGCGGCTGGGTGTGCTCCGCAAGGCCATGGCCCGAGGGCAG
  CTCCTGCTGCAGAGAGACCTGGGCTCCCTCCCTGCGGCAGAGCCACCCCCTGCACCCGAGTCAGGCCTA
  GAGGACAAGCTCAGTGAGCGCCTGGGGGAAGCCTGGGCAGGCCGAGGGGCTGCCTGGTGGGAGAGGCAG
  CAGGGCAGGACCTCGGAGCCCGGGAGACAGACCAGGCGGCCCAAGCGCCGGACCCAGCTGTCCAGCAGC
  TTTTCGCTCAGTGGCCATGTGGATCCCTCAGAGGACACCAGCTCCCCTCATAGCCCTGAGTGGCCACCT
  GCTGATGCTCTGCCCCTGCCCCCCACGACCCCGCCCTCCCAGGAGTTGACTCCGGATGCATGCGCCCAG
  GGCGTCCCATCAGAGCAGCGGCAGATGCTCCGTGACTACATGGCCAAGCTACCACCCCAGAGGGACACC
  CCAGGCTGTGCCACCACACCTCCCCACTCCCAGGCCTCCAGCGTCCGGGCCACTCGCTCCCAGCAGCAC
  ACACCCGTCCTCTCTAGCTCTCAGCCCCTCCGGAAGAAGCCTCGAATGGGCTTCTGA
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_005679
• Insert Size: 2610 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005679.3
• RefSeq Size: 3962 bp
• RefSeq ORF: 2610 bp
• Locus ID: 9013
• UniProt ID: Q15572
• Cytogenetics: 16q24.1
• Protein Families: Transcription Factors
• MW: 95.3 kDa
• Gene Summary: Initiation of transcription by RNA polymerase I requires the formation of a complex composed of the TATA-binding protein (TBP) and three TBP-associated factors (TAFs) specific for RNA polymerase I. This complex, known as SL1, binds to the core promoter of ribosomal RNA genes to position the polymerase properly and acts as a channel for regulatory signals. This gene encodes the largest SL1-specific TAF. Multiple alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2011]
  Transcript Variant: This variant (1) encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.