VNN2 (NM_004665) Human Untagged Clone

CAT#: SC303515

VNN2 (untagged)-Human vanin 2 (VNN2), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: VNN2
• Synonyms: FOAP-4; GPI-80
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_004665 edited
  ATGGTCACTTCCTCTTTTCCAATCTCTGTGGCAGTTTTTGCCCTAATAACCCTGCAGGTT
  GGTACTCAGGACAGTTTTATAGCTGCAGTGTATGAACATGCTGTCATTTTGCCAAATAAA
  ACAGAAACACCAGTTTCTCAGGAGGATGCCTTGAATCTCATGAACGAGAATATAGACATT
  CTGGAGACAGCGATCAAGCAGGCAGCTGAGCAGGGTGCTCGAATCATTGTGACTCCAGAA
  GATGCACTTTATGGATGGAAATTTACCAGGGAAACTGTTTTCCCTTATCTGGAGGATATC
  CCAGACCCTCAGGTGAACTGGATTCCGTGTCAAGACCCCCACAGATTTGGTCACACACCA
  GTACAAGCAAGACTCAGCTGCCTGGCCAAGGACAACTCTATCTATGTCTTGGCAAATTTG
  GGGGACAAAAAGCCATGTAATTCCCGTGACTCCACATGTCCTCCTAATGGCTACTTTCAA
  TACAATACCAATGTGGTGTATAATACAGAAGGAAAACTCGTGGCACGTTACCATAAGTAC
  CACCTGTACTCTGAGCCTCAGTTTAATGTCCCTGAAAAGCCGGAGTTGGTGACTTTCAAC
  ACCGCATTTGGAAGGTTTGGCATTTTCACGTGCTTTGATATATTCTTCTATGATCCTGGT
  GTTACCCTGGTGAAAGATTTCCATGTGGACACCATACTGTTTCCCACAGCTTGGATGAAC
  GTTTTGCCCCTTTTGACAGCTATTGAATTCCATTCAGCTTGGGCAATGGGAATGGGAGTT
  AATCTTCTTGTGGCCAACACACATCATGTCAGCCTAAATATGACAGGAAGTGGTATTTAT
  GCACCAAATGGTCCCAAAGTGTATCATTATGACATGAAGACAGAGTTGGGAAAACTTCTC
  CTTTCAGAGGTGGATTCACATCCCCTATCCTCGCTTGCCTACCCAACAGCTGTTAATTGG
  AATGCCTACGCCACCACCATCAAACCATTTCCAGTACAGAAAAACACTTTCAGGGGATTT
  ATTTCCAGGGATGGGTTCAACTTCACAGAACTTTTTGAAAATGCAGGAAACCTTACAGTC
  TGTCAAAAGGAGCTTTGCTGTCATTTAAGCTACAGAATGTTACAAAAAGAAGAGAATGAA
  GTATACGTTCTAGGAGCTTTTACAGGATTACATGGCCGAAGGAGAAGAGAGTACTGGCAG
  GTCTGCACAATGCTGAAGTGCAAAACTACTAATTTGACAACTTGTGGACGGCCAGTAGAA
  ACTGCTTCTACAAGATTTGAAATGTTCTCCCTCAGTGGCACATTTGGAACAGAGTATGTT
  TTTCCTGAAGTGCTACTTACCGAAATTCATCTGTCACCTGGAAAATTTGAGGTGCTGAAA
  GATGGGCGTTTGGTAAACAAGAATGGATCATCTGGGCCTATACTAACAGTGTCACTCTTT
  GGGAGGTGGTACACAAAGGACTCACTTTACAGCTCATGTGGGACCAGCAATTCAGCAATA
  ACTTACCTGCTAATATTCATATTATTAATGATCATAGCTTTGCAAAATATTGTAATGTTA
  TAG
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004665 unedited
  GGGTTTGGGGGGGTTCACCATTTGGATACGACTCACTATAGGGCGGCCGCGATTCAGATC
  TGGTACCGAGCTCGGGTCCACTAGTAACGGCCGCCAGTGTGCTGGAATTCGCCCTTTATG
  CAAACATTCTCCCCTCTGTAAATACTACATGAATGTTATTTTTGCTTTCAGAAATCACTA
  AACCTTGGCCATGGTCACTTCCTCTTTTCCAATCTCTGTGGCAGTTTTTGCCCTAATAAC
  CCTGCAGGTTGGTACTCAGGACAGTTTTATAGCTGCAGTGTATGAACATGCTGTCATTTT
  GCCAAATAAAACAGAAACACCAGTTTCTCAGGAGGATGCCTTGAATCTCATGAACGAGAA
  TATAGACATTCTGGAGACAGCGATCAAGCAGGCAGCTGAGCAGGGTGCTCGAATCATTGT
  GACTCCAGAAGATGCACTTTATGGATGGAAATTTACCAGGGAAACTGTTTTCCCTTATCT
  GGAGGATATCCCAGACCCTCAGGTGAACTGGATTCCGTGTCAAGACCCCCACAGATTTGG
  TCACACACCAGTACAAGCAAGACTCAGCTGCCTGGCCCAAGACAACTCTATCTATGTCTT
  GGCAAATTTGGNGGACAAAAAGCCATGTAATTCCCGTGACTCCACATGTCCTCCTAATGG
  CTACTTTCAATACAACCAATGTGGTGTATATACAGAAGGAAACTCGTGGCACGTTACCAT
  AGTACACCTGTACTCTGAGCCTCAGTTTATGTCCCTGAAAAGCCGAGTTGGTGACTTTCA
  CCACCCATTTGGAGGTTTGCCATTTTCCGTGCTTGGATAATTCTTCAATGACCCGGTGTA
  ACCTGTGGAAATTTCCTTTGGCACCTACTGTTCCCACGCTTGGT
  
• Restriction Sites: Please inquire
• ACCN: NM_004665
• Insert Size: 1700 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004665.2, NP_004656.2
• RefSeq Size: 2034 bp
• RefSeq ORF: 1563 bp
• Locus ID: 8875
• UniProt ID: O95498
• Cytogenetics: 6q23.2
• Protein Families: Druggable Genome, Transmembrane
• Gene Summary: This gene product is a member of the Vanin family of proteins that share extensive sequence similarity with each other, and also with biotinidase. The family includes secreted and membrane-associated proteins, a few of which have been reported to participate in hematopoietic cell trafficking. No biotinidase activity has been demonstrated for any of the vanin proteins, however, they possess pantetheinase activity, which may play a role in oxidative-stress response. The encoded protein is a GPI-anchored cell surface molecule that plays a role in transendothelial migration of neutrophils. This gene lies in close proximity to, and in same transcriptional orientation as two other vanin genes on chromosome 6q23-q24. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, May 2011]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).