SLIT1 (NM_003061) Human Untagged Clone

CAT#: SC303271

SLIT1 (untagged)-Human slit homolog 1 (Drosophila) (SLIT1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SLIT1
• Synonyms: MEGF4; SLIL1; SLIT-1; SLIT3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC303271 representing NM_003061.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGCGCTGACTCCCGGGTGGGGGTCCTCGGCGGGGCCGGTCCGGCCGGAGCTCTGGCTGCTGCTGTGG
  GCAGCCGCGTGGCGCCTGGGTGCCTCGGCGTGCCCCGCCCTCTGCACCTGCACCGGAACCACGGTGGAC
  TGCCACGGCACGGGGCTGCAGGCCATTCCCAAGAATATACCTCGGAACACCGAGCGCCTGGAACTCAAT
  GGCAACAACATCACTCGGATCCATAAGAATGACTTTGCGGGGCTCAAGCAGCTGCGGGTGCTGCAGCTG
  ATGGAGAACCAGATTGGAGCAGTGGAACGTGGTGCTTTTGATGACATGAAGGAGCTGGAGCGGCTGCGA
  CTGAACCGAAACCAGCTGCACATGTTACCGGAACTGCTGTTCCAGAACAACCAGGCTTTGTCAAGACTG
  GACTTGAGTGAGAACGCCATCCAGGCCATCCCCAGGAAAGCTTTTCGGGGAGCTACGGACCTTAAAAAT
  TTACAGCTGGACAAGAACCAGATCAGCTGCATTGAGGAAGGGGCCTTCCGTGCTCTGCGGGGGCTGGAG
  GTGCTGACCCTGAACAACAACAATATCACCACCATCCCCGTGTCCAGCTTCAACCATATGCCCAAGCTA
  CGGACCTTCCGCCTGCACTCCAACCACCTGTTTTGCGACTGCCACCTGGCCTGGCTCTCGCAGTGGCTG
  AGGCAGCGGCCAACCATCGGGCTCTTCACCCAGTGCTCGGGCCCAGCCAGCCTGCGTGGCCTCAATGTG
  GCAGAGGTCCAGAAGAGTGAGTTCAGCTGCTCAGGCCAGGGAGAAGCGGGGCGCGTGCCCACCTGCACC
  CTGTCCTCCGGCTCCTGCCCGGCCATGTGCACCTGCAGCAATGGCATCGTGGACTGTCGTGGAAAAGGC
  CTCACTGCCATCCCGGCCAACCTGCCCGAGACCATGACGGAGATACGCCTGGAGCTGAACGGCATCAAG
  TCCATCCCTCCTGGAGCCTTCTCACCCTACAGAAAGCTACGGAGGATAGACCTGAGCAACAATCAGATC
  GCTGAGATTGCACCCGACGCCTTCCAGGGCCTCCGCTCCCTGAACTCGCTGGTCCTCTATGGAAACAAG
  ATCACAGACCTCCCCCGTGGTGTGTTTGGAGGCCTATACACCCTACAGCTCCTGCTCCTGAATGCCAAC
  AAGATCAACTGCATCCGGCCCGATGCCTTCCAGGACCTGCAGAACCTCTCACTGCTCTCCCTGTATGAC
  AACAAGATCCAGAGCCTCGCCAAGGGCACTTTCACCTCCCTGCGGGCCATCCAGACTCTGCACCTGGCG
  CAGAACCCTTTCATTTGCGACTGTAACCTCAAGTGGCTGGCAGACTTCCTGCGCACCAATCCCATCGAG
  ACGAGTGGTGCCCGCTGTGCCAGTCCCCGGCGCCTCGCCAACAAGCGCATCGGGCAGATCAAGAGCAAG
  AAGTTCCGGTGCTCAGCCAAAGAGCAGTACTTCATTCCAGGCACGGAGGATTACCAGCTGAACAGCGAG
  TGCAACAGCGACGTGGTCTGTCCCCACAAGTGCCGCTGTGAGGCCAACGTGGTGGAGTGCTCCAGCCTG
  AAGCTCACCAAGATCCCTGAGCGCATCCCCCAGTCCACGGCAGAACTGCGATTGAATAACAATGAGATT
  TCCATCCTGGAGGCCACTGGGATGTTTAAAAAACTTACACATCTGAAGAAAATCAATCTGAGCAACAAC
  AAGGTGTCAGAAATTGAAGATGGGGCCTTCGAGGGCGCAGCCTCTGTGAGCGAGCTGCACCTAACTGCC
  AACCAGCTGGAGTCCATCCGGAGCGGCATGTTCCGGGGTCTGGATGGCTTGAGGACCCTAATGCTGCGG
  AACAACCGCATCAGCTGCATCCACAACGACAGCTTCACGGGCCTGCGCAACGTCCGGCTCCTCTCGCTC
  TACGACAACCAGATCACCACCGTATCCCCAGGAGCCTTCGACACCCTCCAGTCCCTCTCCACACTGAAT
  CTCCTGGCCAACCCTTTCAACTGCAACTGCCAGCTGGCCTGGCTAGGAGGCTGGCTACGGAAGCGCAAG
  ATCGTGACGGGGAACCCGCGATGCCAGAACCCTGACTTTTTGCGGCAGATTCCCCTGCAGGACGTGGCC
  TTCCCTGACTTCAGGTGTGAGGAAGGCCAGGAGGAGGGGGGCTGCCTGCCCCGCCCACAGTGCCCACAG
  GAGTGCGCCTGCCTGGACACCGTGGTCCGATGCAGCAACAAGCACCTGCGGGCCCTGCCCAAGGGCATT
  CCCAAGAATGTCACAGAACTCTATTTGGACGGGAACCAGTTCACGCTGGTTCCGGGACAGCTGTCTACC
  TTCAAGTACCTGCAGCTCGTGGACCTGAGCAACAACAAGATCAGTTCCTTAAGCAATTCCTCCTTCACC
  AACATGAGCCAGCTGACCACTCTGATCCTCAGCTACAATGCCCTGCAGTGCATCCCGCCTTTGGCCTTC
  CAGGGACTCCGCTCCCTGCGCCTGCTGTCTCTCCACGGCAATGACATCTCCACCCTCCAAGAGGGCATC
  TTTGCAGACGTGACCTCCCTGTCTCACCTGGCCATTGGTGCCAACCCCCTATACTGTGACTGCCACCTC
  CGCTGGCTGTCCAGCTGGGTGAAGACTGGCTACAAGGAACCGGGCATTGCTCGTTGTGCTGGGCCCCAG
  GACATGGAGGGCAAGCTGCTCCTCACCACGCCTGCCAAGAAGTTTGAATGCCAAGGTCCTCCAACGCTG
  GCTGTCCAGGCCAAGTGTGATCTCTGCTTGTCCAGTCCGTGCCAGAACCAGGGCACCTGCCACAACGAC
  CCCCTTGAGGTGTACAGGTGCGCCTGCCCCAGCGGCTATAAGGGTCGAGACTGTGAGGTGTCCCTGGAC
  AGCTGTTCCAGTGGCCCCTGTGAAAATGGGGGCACCTGCCATGCACAGGAGGGCGAGGATGCCCCGTTC
  ACGTGCTCCTGTCCCACCGGCTTTGAAGGACCAACCTGTGGGGTGAACACAGATGACTGTGTGGATCAT
  GCCTGTGCCAATGGGGGCGTCTGTGTGGATGGTGTGGGCAACTACACCTGCCAGTGCCCCCTGCAGTAT
  GAGGGAAAGGCCTGTGAGCAGCTGGTGGACTTGTGCTCTCCGGATCTGAACCCATGTCAACACGAGGCC
  CAGTGTGTGGGCACCCCGGATGGGCCCAGGTGTGAGTGCATGCCAGGTTATGCAGGTGACAACTGCAGT
  GAGAACCAGGATGACTGCAGGGACCACCGCTGCCAGAATGGGGCCCAGTGTATGGATGAAGTCAACAGC
  TACTCCTGCCTCTGTGCTGAGGGCTACAGTGGACAGCTCTGTGAGATCCCTCCCCATCTGCCTGCCCCC
  AAGAGCCCCTGTGAGGGGACTGAGTGCCAGAATGGGGCCAACTGTGTGGACCAGGGCAACAGGCCTGTG
  TGCCAGTGCCTCCCAGGCTTCGGTGGCCCTGAGTGTGAGAAGTTGCTCAGTGTCAACTTTGTGGATCGG
  GACACTTACCTGCAGTTCACTGACCTGCAAAACTGGCCACGGGCCAACATCACGTTGCAGGTCTCCACG
  GCAGAGGACAATGGGATCCTTCTGTACAACGGGGACAACGACCACATTGCAGTTGAGCTGTACCAGGGC
  CATGTGCGTGTCAGCTACGACCCAGGCAGCTACCCCAGCTCTGCCATCTACAGTGCTGAGACGATCAAC
  GATGGGCAATTCCACACCGTTGAGCTGGTTGCCTTTGACCAGATGGTGAATCTCTCCATTGATGGCGGG
  AGCCCCATGACCATGGACAACTTTGGCAAACATTACACGCTCAACAGCGAGGCGCCACTCTATGTGGGA
  GGGATGCCCGTGGATGTCAACTCAGCTGCCTTCCGCCTGTGGCAGATCCTCAACGGCACCGGCTTCCAC
  GGTTGCATCCGAAACCTGTACATCAACAACGAGCTGCAGGACTTCACCAAGACGCAGATGAAGCCAGGC
  GTGGTGCCAGGCTGCGAACCCTGCCGCAAGCTCTACTGCCTGCATGGCATCTGCCAGCCCAATGCCACC
  CCAGGGCCCATGTGCCACTGCGAGGCTGGCTGGGTGGGCCTGCACTGTGACCAGCCCGCTGACGGCCCC
  TGCCATGGCCACAAGTGTGTCCATGGGCAATGCGTGCCCCTCGACGCTCTTTCCTACAGCTGCCAGTGC
  CAGGATGGGTACTCGGGGGCACTGTGCAACCAGGCCGGGGCCCTGGCAGAGCCCTGCAGAGGCCTGCAG
  TGCCTGCATGGCCACTGCCAGGCCTCAGGCACCAAGGGGGCACACTGTGTGTGTGACCCCGGCTTTTCG
  GGCGAGCTGTGTGAGCAAGAGTCCGAGTGCCGGGGGGACCCTGTCCGGGACTTTCACCAGGTCCAGAGG
  GGCTATGCCATCTGCCAGACCACGCGCCCCCTGTCATGGGTGGAGTGCCGGGGCTCGTGCCCAGGCCAG
  GGCTGCTGCCAGGGCCTTCGGCTGAAGCGGAGGAAGTTCACCTTTGAGTGCAGCGATGGGACCTCTTTT
  GCCGAGGAGGTGGAAAAGCCCACCAAGTGTGGCTGTGCCCTCTGCGCATAG
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_003061
• Insert Size: 4605 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003061.2
• RefSeq Size: 7949 bp
• RefSeq ORF: 4605 bp
• Locus ID: 6585
• UniProt ID: O75093
• Cytogenetics: 10q24.1
• Protein Families: Druggable Genome
• Protein Pathways: Axon guidance
• MW: 167.9 kDa
• Gene Summary: Thought to act as molecular guidance cue in cellular migration, and function appears to be mediated by interaction with roundabout homolog receptors. During neural development involved in axonal navigation at the ventral midline of the neural tube and projection of axons to different regions (By similarity). SLIT1 and SLIT2 together seem to be essential for midline guidance in the forebrain by acting as repulsive signal preventing inappropriate midline crossing by axons projecting from the olfactory bulb.[UniProtKB/Swiss-Prot Function]