I 309 (CCL1) (NM_002981) Human Untagged Clone

CAT#: SC303257

CCL1 (untagged)-Human chemokine (C-C motif) ligand 1 (CCL1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: I 309
• Synonyms: I-309; P500; SCYA1; SISe; TCA3
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_002981 edited
  AAGCTGCTCCAGGAAGGCCCAAGCCAGACCAGAAGACATGCAGATCATCACCACAGCCCT
  GGTGTGCTTGCTGCTAGCTGGGATGTGGCCGGAAGATGTGGACAGCAAGAGCATGCAGGT
  ACCCTTCTCCAGATGTTGCTTCTCATTTGCGGAGCAAGAGATTCCCCTGAGGGCAATCCT
  GTGTTACAGAAATACCAGCTCCATCTGCTCCAATGAGGGCTTAATATTCAAGCTGAAGAG
  AGGCAAAGAGGCCTGCGCCTTGGACACAGTTGGATGGGTTCAGAGGCACAGAAAAATGCT
  GAGGCACTGCCCGTCAAAAAGAAAATGAGCAGATTTCTTTCCATTGTGGGCTCTGGAAAC
  CACATGGCTTCACCTGTCCCCGAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002981 unedited
  GAGACTTGTATACGACTCCTATAGGGCGGCCGCGAATTCGCCCTTAAGCTGCTCCAGGAA
  GGCCCAAGCCAGACCAGAAGACATGCAGATCATCACCACAGCCCTGGTGTGCTTGCTGCT
  AGCTGGGATGTGGCCGGAAGATGTGGACAGCAAGAGCATGCAGGTACCCTTCTCCAGATG
  TTGCTTCTCATTTGCGGAGCAAGAGATTCCCCTGAGGGCAATCCTGTGTTACAGAAATAC
  CAGCTCCATCTGCTCCAATGAGGGCTTAATATTCAAGCTGAAGAGAGGCAAAGAGGCCTG
  CGCCTTGGACACAGTTGGATGGGTTCAGAGGCACAGAAAAATGCTGAGGCACTGCCCGTC
  AAAAAGAAAATGAGCAGATTTCTTTCCATTGTGGGCTCTGGAAACCACATGGCTTCACCT
  GTCCCCGAACCGTGATGAAGGGCGAATTCAGATCTGGTACCGATATCAAGCTTGTCGACT
  CTAGATTGCGGCCGCGGTCATAGCTGTTTCCTGAACAGATCCCGGGTGGCATCCCTGTGA
  CCCCTCCCCAGTGCCTCTCCTGGCCCTGGAAGTTGCCACTCCAGTGCCCACCAGCCTTGT
  CCTAATAAAATTAAGTTGCATCATTTTGTCTGACTAGGTGTCCTTCTATAATATTATGGG
  GTGGAGGGGGTGGGTATGGGAGCAAGGGGCAAGTTGGGAAGACAACCTGTAGGGCCTGCG
  GGGTCTATTGGGAACCAAGCTGGAGTGCAGTGGCACAATCTTGGCTCACTGCATCTCCGC
  CTCCTGGGTTCAAGCGATTCTCCTGCTCAGCCTCCGAGTTGTTGGATTCCAGGCATGCAT
  GACCAGCTCAGCTAATTTTTGTTTTTTTGTAGAGACGGTTTCACCCATATTGGCCCAGCT
  G
  
• Restriction Sites: Please inquire
• ACCN: NM_002981
• Insert Size: 400 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.

Reference Data

• RefSeq: NM_002981.1, NP_002972.1
• RefSeq Size: 542 bp
• RefSeq ORF: 291 bp
• Locus ID: 6346
• UniProt ID: P22362
• Cytogenetics: 17q12
• Protein Families: Druggable Genome, Secreted Protein
• Protein Pathways: Chemokine signaling pathway, Cytokine-cytokine receptor interaction
• Gene Summary: This antimicrobial gene is one of several chemokine genes clustered on the q-arm of chromosome 17. Chemokines form a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. The superfamily is divided into four subfamilies based on the arrangement of the N-terminal cysteine residues of the mature peptide. This chemokine, a member of the CC subfamily, is secreted by activated T cells and displays chemotactic activity for monocytes but not for neutrophils. It binds to the chemokine (C-C motif) receptor 8. [provided by RefSeq, Sep 2014]