Sec8 (EXOC4) (NM_001037126) Human Untagged Clone

CAT#: SC302849

EXOC4 (untagged)-Human exocyst complex component 4 (EXOC4), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: EXOC4
• Synonyms: SEC8; SEC8L1; Sec8p
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_001037126 edited
  CCAAGATGGCGGCAGAAGCAGCTGGTGGGAAATACAGAAGCACAGTCAGCAAAAGCAAAG
  ACCCCTCGGGGCTGCTCATCTCTGTGATCAGGACTCTGTCTACTAGTGACGATGTCGAAG
  ACAGGGAAAATGAAAAGGGTCGCCTTGAAGAAGCCTACGAGAAATGTGACCGTGACCTGG
  ATGAATTGATTGTACAGCACTACACAGAATTGACGACAGCCATTCGCACATACCAGAGCA
  TCACAGAGCGCATCACTAACTCCCGAAATAAAATAAAGCAGGTAAAAGAGAACCTGCTTT
  CATGCAAGATGCTGCTGCACTGCAAACGGGATGAGCTTCGGAAACTGTGGATTGAAGGAA
  TTGAGCATAAGCATGTCCTGAACTTGTTGGATGAAATTGAGAATATCAAGCAAGTGCCTC
  AAAAGCTGGAACAGTGCATGGCCAGCAAGCACTATCTCAGTGCCACTGACATGTTGGTGT
  CAGCAGTTGAGTCTTTGGAGGGCCCCCTGCTCCAGGTGGAAGGACTGAGTGACCTTCGAC
  TAGAGCTTCACAGCAAGAAGATGAACCTTCACTTGGTTCTCATAGATGAACTACACCGGC
  ACCTGTACATCAAATCGACTAGCCGAGTTGTGCAGCGTAACAAGGAAAAAGGGAAAATCA
  GCTCCCTCGTGAAAGATGCTTCTGTTCCTCTGATTGATGTTACAAACCTCCCTACTCCTC
  GAAAATTCCTTGATACCTCTCACTATTCTACTGCTGGAAGCTCAAGTGTGAGGGAGATAA
  ATCTGCAGGACATCAAGGAAGATTTAGAATTGGATCCAGAGGAAAACAGCACCCTGTTTA
  TGGGTATCCTCATTAAGGGCTTGGCGAAACTGAAGAAGATCCCAGAAACAGTTAAGGCAA
  TCATAGAGCGCTTGGAGCAGGAGTTGAAGCAAATTGTGAAGAGGTCTACAACCCAGGTGG
  CAGACAGTGGCTATCAGCGGGGGGAGAACGTTACTGTGGAGAACCAACCAAGGTTGCTTC
  TAGAACTGCTGGAGTTACTGTTTGACAAGTTTAATGCTGTAGCCGCTGCACACTCTGTGG
  TCCTGGGATACCTGCAGGACACTGTAGTGACTCCACTGACTCAGCAGGAAGATATCAAAC
  TGTATGATATGGCAGATGTATGGGTGAAGATCCAAGATGTTCTACAGATGCTATTAACTG
  AGTACTTGGATATGAAAAATACTCGTACGGCCTCTGAACCATCAGCTCAACTAAGCTATG
  CCAGCACTGGACGAGAGTTTGCAGCCTTTTTTGCCAAGAAGAAACCTCAAAGGCCAAAAA
  ATTCTCTTTTCAAGTTCGAATCGTCCTCCCATGCCATCAGTATGAGCGCCTATCTGCGAG
  AACAGAGAAGGGAGCTCTATAGTCGGAGTGGAGAACTGCAAGGGTAG
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001037126 unedited
  GTGCCTTTGTATACGACTCCTATAGGGCGGCCGAGTAACTCGGCACGAGGCCAAGATGGC
  GGCAGAAGCAGCTTGGTGGGAAATACAGAAGCACAGTCAGCAAAAGCAAAGACCCCTCGG
  GGCTGCTCATCTCTGTGATCAGGACTCTGTCTACTAGTGACGATGTCGAAGACAGGGAAA
  ATGAAAAGGGTCGCCTTGAAGAAGCCTACGAGAAATGTGACCGTGACCTGGATGAATTGA
  TTGTACAGCACTACACAGAATTGACGACAGCCATTCGCACATACCAGAGCATCACAGAGC
  GCATCACTAACTCCCGAAATAAAATAAAGCAGGTAAAATAGAACCTGCTTTCATGCAAGA
  TGCTGCTGCACTGCAAACGGGATGAGCTTCGGAAACTGTGGATTGAAGGAATTGAGCATA
  AGCATGTCCTGAACTTGTTGGATGAAATTGAGAATATCAAGCAAGTGCCTCAAAAGCTGG
  AACAGTGCATGGCCAGCAAGCACTATCTCAGTGCCACTGACATGTTGGTGTCAGCAGTTG
  AGTCTTTGGAGGGCCCCCTGCTCCAGGTGGAAGGACTGAGTGACCTTCGACTAGAGCTTC
  ACAGCAAGAAGATGAACCTTCACTTGGTTCTCATAGATGAACTACACCGGCACCTGTACA
  TCAAATCGACTAGCCGAGTTGTGCAGCGTAACAAGGAAAAAGGGAAAATCAGCTCCCTCG
  TGAAAGATGCTTCTGTTCCTCTGATTGATGTTACAAACCTCCCTACTCCTCGAAAATTCC
  TTGATACCTCTCACTATTCTACTGCTGGAAGCTCAAGTGTGAGGGAGATAAATCTGCAGG
  ACATCAAGGAAGATTTAGAATTGGATCCAGAGGAAAACAGCACCCTGTTTATGGGTATCC
  TCATTAAAGGGCTTGGCCGAAACCTGAAGAAAGAATCCAGAAAAACAGTTTAAAGGC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001037126 unedited
  TCACTCCCAAAACCGAGGCCCGGGAGAGCACTTGGGGAGGGGTCACAGGGATGCCACCCG
  GGATCTGTTCAGGAAACAGCTATGACCGCGGCCGCAATCTAGACTACCCTTGCAGTTCTC
  CACTCCGACTATAGAGCTCCCTTCTCTGTTCTCGCAGATAGGCGCTCATACTGATGGCAT
  GGGAGGACGATTCGAACTTGAAAAGAGAATTTTTTGGCCTTTGAGGTTTCTTCTTGGCAA
  AAAAGGCTGCAAACTCTCGTCCAGTGCTGGCATAGCTTAGTTGAGCTGATGGTTCAGAGG
  CCGTACGAGTATTTTTCATATCCAAGTACTCAGTTAATAGCATCTGTAGAACATCTTGAA
  TCTTCACCCATACATCTGCCATATCATACAGTTTGATATCTTCCTGCTGAGTCAGTGGAG
  TCACTACAGTGTCCTGCAGGTATCCCAGGACCACAGAGTGTGCAGCGGCTACAGCATTAA
  ACTTGTCAAACAGTAACTCCAGCAGTTCTAGAAGCAACCTTGGTTGGTTCTCCACAGTAA
  CGTTCTCCCCCCGCTGATAGCCACTGTCTGCCACCTGGGTTGTAGACCTCTTCACAATTT
  GCTTCAACTCCTGCTCCAAGCGCTCTATGATTGCCTTAACTGTTTCTGGGATCTTCTTCA
  ATTTCGCCAAGCCCTTAATGAGGATACACATAAACAGGGTGCTGTTTTCCTCTGGATCCA
  ATTCTAAATCTTCCTTGATGTCCTGCAGATTTATCTCCCTCACACTTGAGCTTCCAGCAC
  TAGAATAGTGAGAGGTATCAATGAATTTTCGAGGAGTAGGGAGGTTTGGTAACATCATCA
  GAGGAACAGAAGCATCTTTGCACGACGGGAGCTGCTTTTCCCTTTTTTCTTTGTTACGCT
  GCACAACATCGGCTAGTCGATTTGATGTACA
  
• Restriction Sites: Please inquire
• ACCN: NM_001037126
• Insert Size: 1400 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001037126.1, NP_001032203.1
• RefSeq Size: 1620 bp
• RefSeq ORF: 1422 bp
• Locus ID: 60412
• Cytogenetics: 7q33
• Protein Pathways: Tight junction
• Gene Summary: The protein encoded by this gene is a component of the exocyst complex, a multiple protein complex essential for targeting exocytic vesicles to specific docking sites on the plasma membrane. Though best characterized in yeast, the component proteins and functions of exocyst complex have been demonstrated to be highly conserved in higher eukaryotes. At least eight components of the exocyst complex, including this protein, are found to interact with the actin cytoskeletal remodeling and vesicle transport machinery. The complex is also essential for the biogenesis of epithelial cell surface polarity. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (2) lacks multiple exons in the 3' coding region and has a unique 3' UTR, compared to variant 1. This variant encodes isoform b which is much shorter than isoform a.