SLC35F1 (NM_001029858) Human Untagged Clone

CAT#: SC302443

SLC35F1 (untagged)-Human solute carrier family 35, member F1 (SLC35F1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SLC35F1
• Synonyms: C6orf169; dJ230I3.1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC302443 representing NM_001029858.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGATCCCCCCTGAGCAGCCGCAGCAGCAGCTGCAGCCGCCGTCGCCAGCCCCGCCGAACCATGTGGTG
  ACCACCATCGAGAACCTGCCGGCCGAGGGCAGCGGCGGCGGCGGGAGCCTGTCCGCCTCCTCCCGGGCT
  GGCGTGCGCCAGAGGATCCGCAAAGTGCTGAACAGGGAGATGTTAATCTCTGTGGCCCTAGGCCAGGTG
  TTATCCCTCCTTATTTGTGGAATTGGCTTGACTAGCAAGTATCTGTCAGAAGATTTCCACGCCAACACA
  CCAGTCTTCCAGAGTTTCCTCAATTACATTCTTCTCTTCTTGGTCTATACCACCACACTAGCCGTCAGA
  CAAGGAGAAGAAAACCTCCTGGCAATTTTACGACGAAGATGGTGGAAGTACATGATTTTGGGACTCATA
  GACCTGGAAGCAAATTATCTGGTGGTCAAGGCTTACCAATACACAACTCTGACCAGTATCCAGCTCCTG
  GACTGTTTTGTGATCCCAGTCGTGATTTTGCTCTCCTGGTTCTTCCTGCTGATCCGGTACAAGGCTGTG
  CATTTCATCGGCATCGTTGTCTGCATCCTGGGAATGGGCTGCATGGTGGGAGCAGATGTGCTTGTGGGA
  AGACATCAGGGAGCAGGGGAAAATAAGCTGGTAGGGGACCTTCTGGTCTTAGGAGGAGCCACACTCTAT
  GGTATTTCTAACGTCTGGGAAGAATACATCATCCGAACTCTGAGCCGAGTGGAATTCCTGGGAATGATT
  GGTCTCTTTGGAGCATTTTTCAGTGGAATTCAATTAGCTATAATGGAGCATAAGGAACTGTTGAAGGTG
  CCCTGGGACTGGCAAATAGGACTGCTCTACGTTGGCTTTAGTGCCTGCATGTTTGGTCTCTACAGCTTT
  ATGCCAGTCGTCATAAAGAAAACCAGTGCCACTTCAGTCAACCTCTCCTTGCTCACAGCAGACTTGTAC
  AGCCTGTTCTGTGGATTGTTTCTCTTCCACTACAAGTTTTCAGGACTTTATCTCCTGTCTTTCTTCACC
  ATCCTCATTGGGCTGGTGCTCTACTCCTCCACCTCCACCTACATAGCCCAGGACCCCCGAGTGTATAAG
  CAGTTCCGCAATCCTTCAGGACCTGTTGTGGACTTACCGACCACAGCTCAGGTGGAACCCTCAGTCACC
  TACACCAGCCTGGGCCAGGAGACCGAAGAGGAGCCTCATGTTCGTGTGGCCTAG
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001029858
• Insert Size: 1227 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.

Reference Data

• RefSeq: NM_001029858.3
• RefSeq Size: 4852 bp
• RefSeq ORF: 1227 bp
• Locus ID: 222553
• UniProt ID: Q5T1Q4
• Cytogenetics: 6q22.2-q22.31
• Protein Families: Transmembrane
• MW: 45.3 kDa
• Gene Summary: Putative solute transporter.[UniProtKB/Swiss-Prot Function]