PON2 (NM_001018161) Human Untagged Clone

CAT#: SC302202

PON2 (untagged)-Human paraoxonase 2 (PON2), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PON2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_001018161 edited
  GTAAGTTTCTCACTGGAATAGCGAGTGAGAAATGTGTTTGAAGACTAGGGAAACGAGAAG
  GTATGCGGGCTGCTAGAGGGTTTCGGGTGGGTTGTTTGAAAAAGGAAGTGAAGGAATTCA
  AAGTTTCACATCATGAGCCTAATGTAAGGCGTGTCCGGGCCGTCCCGAAGGTATCTGGGG
  AAATAGGTTTGTGAGGACAAAGACCAGGGCCAGGCCCAGGGCGGAGCCTCCGCGGCCCCG
  ACGCAGGGACTCGGCCTAGGCGGAGGACGGGGCGGAGCGCGGCCGGCACCATCGAGCCGG
  GAAGATGGCACCGCCCACGGAGCTGCTGGCCAGGCCGGAGCGAGGCAGCGCGCCCGGCTC
  CCGCGCCATGGGGCGGCTGGTGGCTGTGGGCTTGCTGGGGATCGCGCTGGCGCTCCTGGG
  CGAGAGGCTTCTGGCACTCAGAAATCGACTTAAAGCCTCCAGAGAAGTAGAATCTGTAGA
  CCTTCCACACTGCCACCTGATTAAAGGAATTGAAGCTGGCTCTGAAGATATTGACATACT
  TCCCAATGGTCTGGCTTTTTTTAGTGTGGGTCTAAAATTCCCAGGACTCCACAGCTTTGC
  ACCAGATAAGCCTGGAGGAATACTAATGATGGATCTAAAAGAAGAAAAACCAAGGGCACG
  GGAATTAAGAATCAGTCGTGGGTTTGATTTGGCCTCATTCAATCCACATGGCATCAGCAC
  TTTCATAGACAACGAATTCAAGAATACAGTGGAAATTTTTAAATTTGAAGAAGCAGAAAA
  TTCTCTGTTGCATCTGAAAACAGTCAAACATGAGCTTCTTCCAAGTGTGAATGACATCAC
  AGCTGTTGGACCGGCACATTTCTATGCCACAAATGACCACTACTTCTCTGATCCTTTCTT
  AAAGTATTTAGAAACATACTTGAACTTACACTGGGCAAATGTTGTTTACTACAGTCCAAA
  TGAAGTTAAAGTGGTAGCAGAAGGATTTGATTCAGCAAATGGGATCAATATTTCACCTGA
  TGATAAGTATATCTATGTTGCTGACATATTGGCTCATGAAATTCATGTTTTGGAAAAACA
  CACTAATATGAATTTAACTCAGTTGAAGGTACTTGAGCTGGATACACTGGTGGATAATTT
  ATCTATTGATCCTTCCTCGGGGGACATCTGGGTAGGCTGTCATCCTAATGGCCAGAAGCT
  CTTCGTGTATGACCCGAACAATCCTCCCTCGTCAGAGGTTCTCCGCATCCAGAACATTCT
  ATCTGAGAAGCCTACAGTGACTACAGTTTATGCCAACAATGGGTCTGTTCTCCAAGGAAG
  TTCTGTAGCCTCAGTGTATGATGGGAAGCTGCTCATAGGCACTTTATACCACAGAGCCTT
  GTATTGTGAACTCTAAATTGTACTTTTGGCATGAAAGTGCGATAACTTAACAATTAATTT
  TCTATGAATTGCTAATTCTGAGGGAATTTAACCAGCAACATTGACCCAGAAATGTATGGC
  ATGTGTAGTTAATTTTATTCCAGTAAGGAACGGCCCTTTTAGTTCTTAGAGCACTTTTAA
  CAAAAAAGGAAAATGAACAGGTTCTTTAAAATGCCAAGCAAGGGACAGAAAAGAAAGCTG
  CTTTCGAATAAAGTGAATACATTTTGCACAAAGTAAGCCTCACCTTTGCCTTCCAACTGC
  CAGAACATGGATTCCACTGAAATAGAGTGAATTATATTTCCTTAAAATGTGAGTGACCTC
  ACTTCTGGCACTGTGACTACTATGGCTGTTTAGAACTACTGATAACGTATTTTGATGTTT
  TGTACTTACATCTTTGTTTACCATTAAAAAGTTGGAGTTATATTAAAGACTAACTAAAAT
  CCCAAAAAAAAAAAAAAAAAA
  
• Restriction Sites: Please inquire
• ACCN: NM_001018161
• Insert Size: 1900 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: The ORF of this clone has been fully sequenced and found to be a perfect match to NM_001018161.1.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001018161.1, NP_001018171.1
• RefSeq Size: 1633 bp
• RefSeq ORF: 1029 bp
• Locus ID: 5445
• UniProt ID: Q15165
• Cytogenetics: 7q21.3
• Protein Families: Druggable Genome
• Protein Pathways: Metabolic pathways
• Gene Summary: This gene encodes a member of the paraoxonase gene family, which includes three known members located adjacent to each other on the long arm of chromosome 7. The encoded protein is ubiquitously expressed in human tissues, membrane-bound, and may act as a cellular antioxidant, protecting cells from oxidative stress. Hydrolytic activity against acylhomoserine lactones, important bacterial quorum-sensing mediators, suggests the encoded protein may also play a role in defense responses to pathogenic bacteria. Mutations in this gene may be associated with vascular disease and a number of quantitative phenotypes related to diabetes. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (2) uses an alternate, in-frame splice site in the coding region, compared to variant 1. It encodes isoform 2, which is shorter than isoform 1.