THEMIS (NM_001010923) Human Untagged Clone

CAT#: SC301533

THEMIS (untagged)-Human thymocyte selection associated (THEMIS), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: THEMIS
• Synonyms: C6orf190; C6orf207; GASP; SPOT; TSEPA
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_001010923 edited
  TCCACCCCACTCTGGCTGTAGTGTGACATTCCTGCCTGCCTGAGGAAGAAATGGTGAGCA
  GGGGCTGCAATTGCAGACAAGTGTCACCCAGAAGCCACAAGTTTCTGTGAGCACCAGGTC
  TACAAACTACCCAAGGCATAGCAATGGCATTATCACTGGAAGAATTCGTCCACTCCCTTG
  ACCTCAGGACCCTACCCAGGGTTCTAGAAATCCAGGCAGGCATCTATCTTGAAGGCTCTA
  TTTATGAAATGTTTGGAAATGAATGCTGTTTTTCAACAGGAGAAGTGATTAAAATTACTG
  GTCTCAAAGTTAAGAAGATCATAGCTGAAATTTGTGAGCAGATTGAAGGTTGTGAGTCTC
  TACAGCCATTTGAACTGCCTATGAATTTTCCAGGTCTTTTTAAGATTGTGGCTGATAAAA
  CTCCATACCTTACTATGGAAGAAATCACAAGGACCATTCATATTGGACCAAGTAGACTAG
  GGCATCCTTGCTTCTATCATCAGAAGGATATAAAACTAGAGAACCTCATCATAAAGCAGG
  GTGAGCAAATCATGCTCAACTCAGTTGAAGAGATTGATGGAGAAATAATGGTGAGCTGTG
  CAGTAGCAAGGAATCATCAAACTCACTCATTTAATTTGCCTTTGTCACAAGAAGGAGAAT
  TCTACGAGTGTGAAGATGAACGTATTTACACTCTAAAGGAGATTGTTGAATGGAAGATTC
  CTAAGAACAGAACAAGAACTGTAAACCTTACAGATTTTTCAAATAAGTGGGACTCAACGA
  ATCCATTTCCTAAAGACTTTTATGGTACCCTGATTCTCAAGCCTGTTTATGAAATTCAAG
  GTGTGATGAAATTTCGAAAAGATATAATCCGCATCCTCCCCAGTCTAGATGTCGAAGTCA
  AAGACATCACTGATTCTTACGATGCTAACTGGTTTCTTCAGCTGTTATCAACAGAAGATC
  TTTTTGAAATGACTAGTAAAGAGTTCCCCATAGTGACTGAAGTCATAGAAGCACCTGAAG
  GAAACCACCTGCCCCAAAGCATTTTACAGCCTGGGAAAACCATTGTGATCCACAAAAAGT
  ACCAGGCATCAAGAATCTTAGCTTCAGAAATTAGAAGCAATTTTCCTAAAAGACACTTCT
  TGATCCCCACTAGCTATAAAGGCAAGTTCAAGCGGCGACCGAGGGAGTTCCCAACGGCCT
  ATGACCTAGAGATCGCTAAGAGTGAAAAGGAGCCTCTTCACGTGGTGGCCACCAAAGCGT
  TTCATTCCCCTCATGACAAGCTGTCATCCGTATCTGTTGGGGACCAGTTTCTGGTGCATC
  AGTCAGAGACGACTGAAGTCCTCTGTGAGGGAATAAAAAAAGTGGTGAATGTTCTGGCCT
  GTGAAAAAATCCTCAAAAAGTCCTATGAGGCTGCGCTGCTCCCTTTGTACATGGAAGGAG
  GTTTTGTAGAGGTGATTCATGATAAGAAACAGTACCCGATTTCTGAGCTCTGTAAACAGT
  TCCGTTTGCCCTTCAATGTGAAGGTGTCTGTCAGGGATCTTTCCATTGAAGAGGACGTGT
  TGGCTGCCACACCAGGACTGCAGTTGGAGGAGGACATTACAGACTCTTACCTACTCATAA
  GTGACTTTGCCAACCCCACGGAGTGCTGGGAAATTCCTGTGGGCCGCTTGAATATGACTG
  TTCAGTTAGTTAGTAATTTCTCTAGGGATGCAGAACCATTTCTAGTCAGGACTCTGGTAG
  AAGAGATCACTGAAGAGCAATATTACATGATGCGGAGATATGAAAGCTCAGCCTCACATC
  CCCCACCTCGCCCTCCGAAACACCCCTCAGTAGAGGAAACAAAGTTAACCCTGCTAACCT
  TAGCAGAAGAAAGGACGGTAGACCTGCCCAAGTCTCCCAAGCGTCATCACGTAGACATAA
  CCAAGAAACTTCACCCAAATCAAGCTGGCCTGGATTCAAAAGTACTGATTGGTAGTCAGA
  ATGATTTGGTGGATGAAGAGAAAGAAAGGAGCAACCGTGGGGCCACAGCAGTAGCAGAAA
  CATTCAAAAATGAAAAACATCAAAAATAACAAGATGTGACAGAAGCCACTTAGGCAGCAA
  ACATAAATGTTGCAGTGAAAAAAGAAGCTAGCCTTCTAGCTGAAAAACGAGTATTCCCCA
  ATGGACTCCAGAAGAAACTTGATTCATCGCTGCAAAGGAAAGAACAACCTTAAAACTTTT
  AACAGATAAAACTTACAGAAACCTATGATATAGAATTCATATAGTCTATTCTGTTGTGTC
  TAAATCTGTAGGCATTGTGTTGTTGTTCTTTAGGACGTATTTATTTAACTTGCACATTTT
  TTCAGATTCTTATTTCTACTACCAACAACTAAGTAATTGGGAAATAATTCTGTATTTCAG
  TTTCTGAGTAAAACCAGTCTGAAATAGGATAAAAGCCACCAAATATTTTCTTTTTTTTCC
  AGAATTTGTTTTGCCATTTTTTAGTGCTATCATCATTCCTAACAAGACTAACTTACAGAA
  AAATAATTATATCTGACTGATTTAAAATGTTCAGGTTTCTTATCCAAATCCCTTGGAACT
  ATGGAAAGGAGTTTGATTTCACATTCACAGTGTATTTACAAAATACGCTGTGTCATAAAT
  ATGTTTGAATTCCAACAGCCAAAGCCATTGA
  
• Restriction Sites: Please inquire
• ACCN: NM_001010923
• Insert Size: 2700 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The ORF of this clone has been fully sequenced and found to contain one SNP compared with NM_001010923.1.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001010923.1, NP_001010923.1
• RefSeq Size: 4039 bp
• RefSeq ORF: 1926 bp
• Locus ID: 387357
• UniProt ID: Q8N1K5
• Cytogenetics: 6q22.33
• Gene Summary: This gene encodes a protein that plays a regulatory role in both positive and negative T-cell selection during late thymocyte development. The protein functions through T-cell antigen receptor signaling, and is necessary for proper lineage commitment and maturation of T-cells. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2009]
  Transcript Variant: This variant (2) lacks an alternate in-frame exon in the central coding region, compared to variant 1, resulting in an isoform (2) that is shorter than isoform 1.