ST8SIA6 (NM_001004470) Human Untagged Clone

CAT#: SC300704

ST8SIA6 (untagged)-Human ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 6 (ST8SIA6)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ST8SIA6
• Synonyms: SIA8F; SIAT8-F; SIAT8F; ST8SIA-VI; ST8SiaVI
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC300704 representing NM_001004470.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGCGGCCGGGGGGCGCACTGCTCGCCCTGCTCGCCAGCCTGCTGCTGCTGCTGCTGCTGCGCCTGCTC
  TGGTGCCCGGCAGACGCGCCCGGCCGCGCCAGGATTCTGGTGGAGGAAAGCAGGGAGGCCACCCACGGC
  ACCCCCGCAGCGCTGAGGACGCTCCGGAGCCCGGCGACCGCGGTACCGCGCGCCACTAACAGCACATAT
  CTGAATGAGAAGTCGCTCCAACTGACGGAGAAATGCAAAAATCTGCAATATGGCATTGAGTCTTTCTCT
  AACAAAACGAAAGGGTATTCAGAGAACGACTACCTTCAGATTATCACAGATATACAGAGTTGTCCATGG
  AAACGGCAAGCAGAAGAATATGCAAATTTTAGAGCCAAACTTGCTTCCTGCTGTGATGCTGTTCAAAAC
  TTTGTTGTTTCTCAGAATAACACTCCAGTTGGGACTAATATGAGTTACGAGGTGGAAAGCAAAAAAGAA
  ATCCCAATTAAGAAGAACATTTTTCATATGTTTCCAGTGTCCCAGCCTTTTGTGGACTACCCTTATAAT
  CAGTGTGCAGTGGTCGGAAATGGGGGAATTCTGAATAAGTCTCTCTGTGGAACTGAAATAGATAAATCC
  GACTTCGTTTTTAGGTGTAACCTACCCCCAACCACAGGAGATGTTAGTAAAGATGTTGGCAGTAAAACA
  AATCTTGTGACTATAAATCCAAGCATCATAACTCTGAAATATGGGAACTTAAAGGAAAAAAAAGCCCTA
  TTTCTGGAGGACATTGCAACCTATGGAGATGCATTTTTTCTTCTGCCAGCATTTTCCTTCAGGGCCAAC
  ACGGGTACCTCTTTCAAAGTATACTACACGCTCGAAGAGTCTAAAGCAAGACAAAAGGTTCTATTTTTC
  CATCCCAAGTACCTGAAAGATCTGGCCCTTTTCTGGAGAACTAAAGGTGTGACTGCATACCGCTTGTCC
  ACCGGCTTGATGATCACAAGTGTTGCAGTGGAACTGTGTAAAAATGTGAAGCTGTATGGATTCTGGCCC
  TTCTCTAAAACTGTAGAAGACATACCTGTCAGCCATCACTATTATGACAACAAGCTACCTAAACATGGT
  TTCCATCAGATGCCCAAAGAATACAGCCAGATCCTCCAACTTCACATGAAAGGAATCCTCAAACTGCAA
  TTTAGCAAATGTGAAGTCGCCTAA
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001004470
• Insert Size: 1197 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001004470.1
• RefSeq Size: 1398 bp
• RefSeq ORF: 1197 bp
• Locus ID: 338596
• UniProt ID: P61647
• Cytogenetics: 10p12.33
• Protein Families: Transmembrane
• MW: 44.8 kDa
• Gene Summary: This gene encodes a member of the glycosyltransferase 29 protein family. Members of this protein family synthesize sialylglycoconjugates. Sialylation may contribute to multidrug resistance in cancer cells. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2016]
  Transcript Variant: This variant (1) encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.