C18orf1 (LDLRAD4) (NM_001003675) Human Untagged Clone

CAT#: SC300505

LDLRAD4 (untagged)-Human chromosome 18 open reading frame 1 (C18orf1), transcript variant c2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: LDLRAD4
• Synonyms: C18orf1
• Vector: pCMV6 series
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001003675, the custom clone sequence may differ by one or more nucleotides
  ATGTCCAGTGACCACCTGAACAACAGCACACTGAAGGAGGCTCAGTTCAAAGACCTGTTC
  TTAAAAAAAGCGGAGCTGGAGTTCGCCCAAATCATCATCATCGTCGTGGTGGTCACGGTG
  ATGGTGGTGGTCATCGTCTGCCTGCTGAACCACTACAAAGTCTCCACGCGGTCCTTCATC
  AACCGCCCGAACCAGAGCCGGAGGCGGGAGGACGGGCTGCCGCAGATCATGCATGCCCCG
  CGGTCCAGGGACAGGTTCACAGCGCCGTCCTTCATCCAGAGGGATCGCTTCAGCCGCTTC
  CAGCCCACCTACCCCTATGTGCAGCACGAGATTGATCTTCCTCCCACCATCTCCCTGTCC
  GACGGTGAAGAGCCACCTCCTTACCAGGGGCCCTGCACCCTGCAGCTCCGGGACCCTGAA
  CAGCAGATGGAACTCAACCGAGAGTCCGTGAGGGCCCCACCCAACCGAACCATATTTGAC
  AGTGATTTAATAGACATTGCTATGTATAGCGGGGGTCCATGCCCACCCAGCAGCAACTCG
  GGCATCAGTGCAAGCACCTGCAGCAGTAACGGGAGGATGGAGGGGCCACCCCCCACATAC
  AGCGAGGTGATGGGCCACCACCCAGGCGCCTCTTTCCTCCATCACCAGCGCAGCAACGCA
  CACAGGGGCAGCAGACTGCAGTTTCAGCAGAACAATGCAGAGAGCACAATAGTACCCATC
  AAAGGCAAAGATAGGAAGCCTGGGAACCTGGTCTGA
  
• Restriction Sites: Please inquire
• ACCN: NM_001003675
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001003675.1, NP_001003675.1
• RefSeq Size: 8890 bp
• RefSeq ORF: 756 bp
• Locus ID: 753
• UniProt ID: O15165
• Cytogenetics: 18p11.21
• Protein Families: Druggable Genome, Transmembrane
• Gene Summary: Functions as a negative regulator of TGF-beta signaling and thereby probably plays a role in cell proliferation, differentiation, apoptosis, motility, extracellular matrix production and immunosuppression. In the canonical TGF-beta pathway, ZFYVE9/SARA recruits the intracellular signal transducer and transcriptional modulators SMAD2 and SMAD3 to the TGF-beta receptor. Phosphorylated by the receptor, SMAD2 and SMAD3 then form a heteromeric complex with SMAD4 that translocates to the nucleus to regulate transcription. Through interaction with SMAD2 and SMAD3, LDLRAD4 may compete with ZFYVE9 and SMAD4 and prevent propagation of the intracellular signal.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (c2) contains an alternate 5' terminal exon, differs in its 5' UTR, lacks a portion of the 5' coding region, initiates translation at an alternate start codon, and lacks an alternate in-frame exon in the central coding region, compared to variant a1. The encoded isoform (gamma 2) is shorter and has a distinct N-terminus, compared to isoform alpha 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.