Cytokeratin 2 (KRT2) (NM_000423) Human Untagged Clone

CAT#: SC300067

KRT2 (untagged)-Human keratin 2 (KRT2)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Cytokeratin 2
• Synonyms: CK-2e; K2e; KRT2A; KRT2E; KRTE
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC300067 representing NM_000423.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGAGTTGTCAGATCTCTTGCAAATCTCGAGGAAGAGGAGGAGGTGGAGGAGGATTCCGGGGCTTCAGC
  AGCGGCTCAGCTGTGGTGTCTGGTGGAAGCCGGAGATCAACTTCCAGCTTCTCCTGCTTGAGCCGCCAT
  GGTGGTGGTGGCGGGGGCTTCGGTGGAGGCGGCTTTGGCAGTCGGAGTCTTGTTGGCCTTGGAGGGACC
  AAGAGCATCTCCATTAGTGTGGCTGGAGGAGGTGGTGGCTTTGGCGCCGCTGGTGGATTTGGTGGCAGA
  GGAGGTGGTTTTGGAGGCGGCAGCAGCTTTGGAGGTGGCAGCGGCTTCAGTGGTGGTGGTTTCGGTGGA
  GGCGGCTTTGGTGGAGGCCGCTTTGGAGGTTTTGGGGGCCCTGGTGGTGTTGGAGGTTTAGGGGGTCCT
  GGTGGCTTTGGGCCTGGAGGATACCCTGGTGGCATCCACGAAGTCTCTGTCAACCAGAGCCTCCTGCAG
  CCTCTCAACGTGAAAGTTGACCCAGAGATCCAGAATGTGAAGGCCCAAGAGCGTGAGCAGATCAAAACT
  CTCAACAACAAATTTGCCTCCTTCATTGACAAGGTGCGGTTCTTGGAGCAGCAGAACCAGGTGTTACAG
  ACCAAATGGGAGCTGCTACAACAAATGAATGTTGGCACCCGCCCCATCAACCTGGAGCCCATCTTCCAG
  GGGTATATCGACAGCCTCAAGAGATATCTGGATGGGCTCACTGCAGAAAGAACATCACAGAATTCAGAG
  CTGAATAACATGCAGGATCTTGTGGAGGATTATAAGAAGAAGTATGAGGATGAAATCAATAAGCGCACA
  GCTGCTGAGAATGATTTTGTGACGCTTAAAAAGGACGTGGACAATGCCTACATGATAAAGGTGGAGTTG
  CAGTCCAAGGTGGACCTGCTGAACCAGGAAATTGAGTTTCTGAAAGTTCTCTATGATGCGGAGATATCC
  CAGATACATCAGAGTGTCACTGACACCAACGTCATCCTCTCCATGGACAACAGCCGCAACCTGGACTTG
  GATAGCATCATCGCCGAGGTCAAGGCCCAGTATGAGGAGATCGCCCAGAGGAGCAAGGAAGAAGCGGAG
  GCCCTGTACCACAGCAAGTATGAGGAGCTCCAGGTGACTGTCGGGAGACATGGAGACAGCCTGAAAGAG
  ATCAAGATAGAGATCAGCGAGCTGAACCGCGTGATCCAGAGGCTGCAGGGGGAGATCGCACATGTGAAG
  AAGCAGTGTAAGAATGTGCAAGATGCCATCGCAGATGCCGAGCAGCGTGGGGAGCATGCCCTCAAGGAT
  GCCAGGAACAAGTTGAATGACCTGGAGGAGGCCCTGCAGCAGGCCAAGGAGGACTTGGCGCGGCTGCTG
  CGTGACTACCAGGAGCTGATGAACGTGAAGCTGGCCCTAGATGTGGAGATCGCCACCTACCGCAAACTG
  CTGGAGGGCGAGGAGTGCAGGATGTCTGGAGACCTCAGCAGCAATGTGACTGTGTCTGTGACAAGCAGC
  ACCATTTCATCAAATGTGGCATCCAAGGCTGCCTTTGGAGGTTCTGGAGGTAGAGGGTCCAGTTCCGGA
  GGAGGATACAGCTCTGGAAGCAGCAGTTATGGCTCTGGAGGCCGACAGTCTGGCTCCAGAGGCGGTAGT
  GGAGGAGGAGGTTCTATCTCTGGAGGAGGATATGGCTCTGGCGGTGGTTCTGGAGGAAGATACGGATCT
  GGTGGTGGCTCTAAGGGAGGGTCCATCTCTGGAGGAGGATATGGCTCTGGAGGTGGAAAACACAGCTCT
  GGAGGTGGCTCTAGAGGAGGCTCCAGCTCTGGAGGAGGATATGGCTCTGGAGGTGGGGGTTCTAGCTCT
  GTAAAGGGTAGCTCAGGTGAAGCTTTTGGTTCCAGCGTGACCTTCTCTTTTAGATAA
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_000423
• Insert Size: 1920 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000423.2
• RefSeq Size: 2459 bp
• RefSeq ORF: 1920 bp
• Locus ID: 3849
• UniProt ID: P35908
• Cytogenetics: 12q13.13
• MW: 65.4 kDa
• Gene Summary: The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is expressed largely in the upper spinous layer of epidermal keratinocytes and mutations in this gene have been associated with bullous congenital ichthyosiform erythroderma. The type II cytokeratins are clustered in a region of chromosome 12q12-q13. [provided by RefSeq, Jul 2008]