HERC4 (NM_015601) Human Untagged Clone

CAT#: SC128201

HERC4 (untagged)-Human hect domain and RLD 4 (HERC4), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: HERC4
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC128201 sequence for NM_015601 edited (data generated by NextGen Sequencing)
  ATGTTGTGCTGGGGAAATGCATCCTTTGGGCAGCTAGGTTTGGGTGGAATTGATGAAGAA
  ATTGTACTAGAGCCCAGAAAAAGTGACTTCTTTATAAATAAAAGGGTCCGAGATGTAGGA
  TGTGGACTCAGACATACTGTGTTTGTTCTGGATGATGGAACAGTGTACACATGTGGATGT
  AATGATCTAGGACAGCTAGGTCATGAAAAATCCAGAAAGAAACCAGAGCAGGTTGTTGCC
  CTGGATGCCCAAAATATTGTAGCTGTTTCATGTGGAGAAGCTCATACGTTAGCGCTAAAT
  GACAAAGGCCAGGTGTATGCTTGGGGTCTCGATTCTGATGGACAGCTTGGCCTGGTAGGA
  TCAGAGGAATGCATCAGAGTACCCAGAAATATTAAAAGTTTGTCAGATATCCAGATTGTA
  CAGGTTGCTTGTGGTTACTATCATTCACTTGCACTTTCTAAAGCAAGTGAAGTCTTCTGT
  TGGGGACAGAATAAATATGGCCAATTGGGTTTAGGTACTGACTGTAAAAAGCAAACTTCA
  CCGCAGCTGCTTAAGTCTTTGCTTGGAATCCCTTTCATGCAAGTTGCAGCAGGAGGAGCC
  CATAGTTTTGTACTCACCCTTTCTGGAGCTATCTTTGGATGGGGACGCAACAAGTTTGGT
  CAGCTAGGTCTTAATGATGAAAATGATAGGTATGTTCCTAATTTACTAAAGTCACTAAGA
  TCTCAGAAAATAGTTTATATTTGTTGTGGAGAAGATCATACTGCTGCTCTAACCAAGGAA
  GGTGGAGTGTTTACTTTTGGAGCTGGAGGGTATGGTCAGTTGGGCCATAATTCTACCAGT
  CATGAAATAAACCCAAGGAAAGTTTTTGAACTTATGGGAAGCATTGTCACTGAGATTGCT
  TGTGGACGGCAGCACACTTCTGCTTTTGTTCCTTCATCAGGACGAATTTACTCTTTTGGG
  CTTGGTGGTAATGGGCAGCTGGGAACCGGTTCAACAAGCAACAGGAAAAGTCCCTTTACT
  GTAAAAGGAAATTGGTACCCCTATAATGGGCAGTGTCTACCAGATATTGATTCTGAAGAA
  TATTTCTGTGTAAAAAGATTTTTCTCAGGGGGAGATCAAAGCTTTTCACATTACTCTAGT
  CCCCAGAACTGTGGGCCACCAGATGACTTCAGATGTCCCAATCCGACAAAGCAGATCTGG
  ACAGTGAATGAAGCTCTAATTCAGAAATGGCTGAGCTATCCTTCTGGAAGGTTTCCTGTG
  GAGATAGCCAATGAGATAGATGGAACGTTTTCTTCCTCTGGTTGCCTAAATGGAAGTTTT
  TTAGCTGTTAGCAATGATGATCACTATAGAACAGGTACCAGATTTTCAGGGGTTGATATG
  AATGCTGCTAGGCTTTTATTCCACAAACTTATACAACCTGATCATCCGCAGATATCTCAG
  CAGGTGGCAGCTAGTTTGGAAAAGAATCTTATTCCTAAACTGACTAGCTCCTTACCTGAT
  GTTGAAGCATTGAGGTTTTATCTTACTCTACCAGAATGTCCCCTGATGAGTGATTCCAAC
  AATTTCACAACAATAGCAATTCCCTTTGGTACAGCTCTTGTGAACCTAGAAAAGGCACCA
  CTGAAAGTACTTGAAAACTGGTGGTCAGTACTTGAACCTCCACTATTCCTCAAGATAGTA
  GAACTTTTTAAGGAAGTTGTGGTACATCTTTTGAAACTCTACAAGATCGGTATTCCCCCT
  TCTGAAAGAAGAATTTTCAACAGTTTTCTTCATACTGCATTAAAGGTTTTAGAAATACTA
  CATAGGGTAAATGAGAAAATGGGACAGATTATACAGTATGATAAATTTTATATACATGAA
  GTACAAGAATTGATAGACATAAGAAATGATTATATCAACTGGGTCCAACAGCAGGCCTAT
  GGAATGTTGGCAGATATCCCTGTTACAATCTGTACATATCCATTTGTATTTGATGCCCAA
  GCAAAAACTACTCTGTTACAGACCGATGCAGTCTTACAGATGCAGATGGCTATTGATCAG
  GCCCACAGGCAGAATGTCTCCTCTCTTTTTCTCCCAGTGATTGAATCTGTGAATCCCTGC
  TTAATTCTAGTGGTGCGTAGAGAAAATATTGTAGGAGATGCAATGGAAGTCCTTAGGAAA
  ACAAAGAACATAGATTACAAGAAGCCACTCAAGGTTATATTTGTTGGAGAAGATGCTGTG
  GATGCAGGAGGGGTGCGCAAAGAATTTTTCTTGCTCATCATGAGGGAATTATTGGATCCT
  AAATACGGCATGTTTAGGTATTATGAAGATTCCAGGCTCATTTGGTTTTCTGATAAGACA
  TTTGAAGACAGTGATTTGTTCCATTTGATTGGTGTTATCTGTGGCTTAGCAATTTATAAT
  TGTACCATTGTGGACCTCCATTTTCCTTTGGCTTTATATAAGAAACTACTGAAAAAGAAG
  CCATCCTTGGATGATTTGAAAGAACTAATGCCTGATGTTGGGAGAAGCATGCAACAGTTA
  CTGGATTATCCAGAAGATGACATAGAGGAAACATTTTGTCTTAATTTTACGATCACAGTT
  GAAAACTTTGGTGCAACAGAAGTGAAAGAGCTGGTTCTAAATGGTGCAGACACAGCTGTT
  AACAAACAAAATCGGCAAGAGTTTGTCGATGCTTATGTGGATTACATATTCAATAAATCA
  GTGGCTTCCTTATTTGATGCTTTTCATGCGGGCTTTCATAAGGTCTGTGGAGGAAAAGTC
  CTTCTGCTCTTTCAGCCTAATGAACTACAAGCAATGGTCATTGGAAATACAAATTATGAT
  TGGAAGGAACTGGAAAAGAATACAGAATACAAAGGGGAATATTGGGCAGAACATCCTACG
  ATAAAAATTTTTTGGGAAGTATTTCACGAATTACCATTGGAAAAGAAGAAACAGTTTCTG
  TTATTTTTGACAGGTAGTGATCGCATTCCTATTCTTGGTATGAAGAGTCTGAAACTAGTC
  ATCCAGTCCACAGGAGGTGGTGAGGAGTATCTCCCAGTTTCCCATACTTGTTTTAATCTT
  CTGGATCTTCCAAAATATACAGAAAAAGAAACTCTACGCTCTAAACTGATCCAAGCTATT
  GATCACAATGAAGGCTTCAGTTTAATATAA
  
  Clone variation with respect to NM_015601.3
  1011 c=>t;1099 a=>t
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_015601 unedited
  GGGTGGCANAATTTGTAATACGAACTCACTATAGGGCGGCCGCGAATTCGCACGAGGCCA
  CCTCCCCCTCTGCCTTCTCCTACCCAGTCTCTTCCCCAACTCTCCCCACCCCTGGTGCTC
  TCCGCCCCGCTTGCCTCCCGTTCTTCCCCGCCCGCTCCATCCGGGTCGCTGACGGCTGTC
  TCTGGACTGGACAGCAGTGGCGTCCGCTTTTCTCTAAGGAACCCGATAAGAACTCCATTT
  TCTGTTGATGTGGAGACATAGGGTACCTCCAACTTTCCGGGGTTTCAGAAGAGAATTTTT
  TATTGAAACTGGAAGACCAAAATAATTACAAGCATGTTGTGCTGGGGAAATGCATCCTTT
  GGGCAGCTAGGTTTGGGTGGAATTGATGAAGAAATTGTACTAGAGCCCAGAAAAAGTGAC
  TTCTTTATAAATAAAAGGGTCCGAGATGTAGGATGTGGACTCAGACATACTGTGTTTGTT
  CTGGATGATGGAACAGTGTACACATGTGGATGTAATGATCTAGGACAGCTAGGTCATGAA
  AAATCCAGANAGAAACCAGAGCAGGTTGTTGCCCTGGATGCCCAAAATATTGTAGCTGTT
  TCATGTGGAGAAGCTCATACGTTAGCGCTAAATGACAAAGGCCAGGTGTATGCTTGGGGT
  CTCGATTCTGATGGACAGCTTGGCCTGGTAGGATCAGAGGAATGCATCANAGTACCCAAA
  ATATNTAAAGTTTGTCAGATATCCAGATTGTACAGGTTGCTTGTGGTTACTATCATTCAC
  TTGCACTTTCTAAAGCAAGTGAAGTCCTTCTGTTGGGGACAGAATAAATATTGGCCAATT
  GGTTTAAGTACTGACTGTAAAAAGCAAACTTTACCGCAGCTGCTTTAGTCTTTGCCTGGG
  ATCCTTTCATGCAAGNTGCACCAGAAGGACCCTC
  
  3' Read Nucleotide Sequence

  >OriGene 3' genomic read for NM_015601 unedited
  NGATCGATTTTTTCTTTTTTTTTTTAAATATTAAAACAATAAATCTTTATTAAACAGTTT
  TCACGGCATTTTAAAATAAATTTAAAAAATAAATATTTGCATTATAGCAGAAAGTCACAT
  GTATTGCATCAACTCACTTTTACAATTTAATGGCATGAGACAACTAAGCATCAGCACCAT
  AAAACTGTTAAGATTTGTTTCTTAAATTCAAGGAAATAGCTCTAAAGCACTGCAGCATCA
  ATAATTTTGAAAGCTATAACCCAGTGATTGGTGCTCATAGTAGCCAATGTTTCTTTTTAT
  TACTGGCAACTGTGGAGACAGAGTTCTTCCAGTACTAAATATTAATAAGTTCTCTGATCG
  ACAATCCCCCATCTATCAGTGTGTCAGGATTCACAAAGAAAAGAACTGCAGGTTATGTAG
  TATATTCCCTATCTGTGATTTAGGTTTGCACAAAGACACTTATTCTAGCAAAAAAGAAAA
  GATAGTGTTTCCACCATGCTACCTTTTTTTTCTTTTTATAGAGTCTCAGAGTAAGATTTA
  AATCATAATTTTTAACCTTTAAAAGTCTGTTCAAGTTCAATTACAACCATAGAAACTGTA
  CTGGTATCAGAACATAACTATTTTATTACAAAACTTAACATTATTTACAAAATGAAAAAA
  TAATCAAATGACTATTGCAGGCCAAAGTTAAAGGTTTTTTCACTCAATGATTGGAAGAAA
  ATTNAAGCATATTTTCATGCACTCACACCAGATCATTTCTGAAATATGCAAACTCTTAAA
  ATCATGTTAGTAAAACTTAATGTATTCATATACTTGCTAGTTTATTAGAAGAGGTCAAAA
  AAATCCATGGTCTGTACATAATATAACAAGTTGTA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_015601
• Insert Size: 4700 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_015601.2, NP_056416.2
• RefSeq Size: 3842 bp
• RefSeq ORF: 3150 bp
• Locus ID: 26091
• UniProt ID: Q5GLZ8
• Cytogenetics: 10q21.3
• Protein Families: Druggable Genome
• Protein Pathways: Ubiquitin mediated proteolysis
• Gene Summary: HERC4 belongs to the HERC family of ubiquitin ligases, all of which contain a HECT domain and at least 1 RCC1 (MIM 179710)-like domain (RLD). The 350-amino acid HECT domain is predicted to catalyze the formation of a thioester with ubiquitin before transferring it to a substrate, and the RLD is predicted to act as a guanine nucleotide exchange factor for small G proteins (Hochrainer et al., 2005 [PubMed 15676274]).[supplied by OMIM, Mar 2008]
  Transcript Variant: This variant (2) lacks an alternate in-frame exon compared to variant 1. The resulting isoform (b) has the same N- and C-termini but is shorter compared to isoform a.