TFII I (GTF2I) (NM_033001) Human Untagged Clone

CAT#: SC127927

GTF2I (untagged)-Human general transcription factor IIi (GTF2I), transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TFII I
• Synonyms: BAP135; BTKAP1; DIWS; GTFII-I; IB291; SPIN; TFII-I; WBS; WBSCR6
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_033001, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGCCCAAGTTGCAATGTCCACCCTCCCCGTTGAAGATGAGGAGTCCTCGGAGAGCAGGATGGTGGTGA
  CATTCCTCATGTCAGCTCTCGAGTCCATGTGTAAAGAACTGGCCAAGTCCAAAGCCGAAGTGGCCTGCAT
  TGCAGTGTATGAAACAGACGTGTTTGTCGTCGGAACTGAAAGAGGACGTGCTTTTGTCAATACCAGAAAG
  GATTTTCAAAAAGATTTTGTAAAATATTGTGTTGAAGAAGAAGAAAAAGCTGCAGAGATGCATAAAATGA
  AATCTACAACCCAGGCAAATCGGATGAGTGTAGATGCTGTAGAAATTGAAACACTCAGAAAAACAGTTGA
  GGACTATTTCTGCTTTTGCTATGGGAAAGCTTTAGGCAAATCCACAGTGGTACCTGTACCATATGAGAAG
  ATGCTGCGAGACCAGTCGGCTGTGGTAGTGCAGGGGCTTCCGGAAGGTGTTGCCTTTAAACACCCCGAGA
  ACTATGATCTTGCAACCCTGAAATGGATTTTGGAGAACAAAGCAGGGATTTCATTCATCATTAAGAGACC
  TTTTTTAGAGCCAAAGAAGCATGTAGGTGGTCGTGTGATGGTAACAGATGCTGACAGGTCAATACTATCT
  CCAGGTGGAAGTTGTGGCCCCATCAAAGTGAAAACTGAACCCACAGAAGATTCTGGCATTTCCCTGGAAA
  TGGCAGCTGTGACAGTAAAGGAAGAATCAGAAGATCCTGATTATTATCAATATAACATTCAAGCAGGCCC
  TTCTGAAACTGATGATGTTGATGAAAAACAGCCCCTATCGAAGCCTTTGCAAGGAAGCCACCATTCTTCA
  GAGGGCAATGAAGGCACAGAAATGGAAGTACCAGCAGAAGATGATGATTATTCTCCACCGTCTAAGAGAC
  CAAAGGCCAATGAGCTACCGCAGCCACCAGTCCCGGAACCCGCCAATGCTGGGAAGCGGAAAGTGAGGGA
  GTTCAACTTCGAGAAATGGAATGCTCGCATCACTGATCTACGTAAACAAGTTGAAGAATTGTTTGAAAGG
  AAATATGCTCAAGCCATAAAAGCCAAAGGTCCGGTGACGATCCCGTACCCTCTTTTCCAGTCTCATGTTG
  AAGATCTTTATGTAGAAGGACTTCCTGAAGGAATTCCTTTTAGAAGGCCATCTACTTACGGAATTCCTCG
  CCTGGAGAGGATATTACTTGCAAAGGAAAGGATTCGTTTTGTGATTAAGAAACATGAGCTTCTGAATTCA
  ACACGTGAAGATTTACAGCTTGATAAGCCAGCTTCAGGAGTAAAGGAAGAATGGTATGCCAGAATCACTA
  AATTAAGAAAGATGGTGGATCAGCTTTTCTGCAAAAAATTTGCGGAAGCCTTGGGGAGCACTGAAGCCAA
  GGCTGTACCGTACCAAAAATTTGAGGCACACCCGAATGATCTGTACGTGGAAGGACTGCCAGAAAACATT
  CCTTTCCGAAGTCCCTCATGGTATGGAATCCCAAGGCTGGAAAAAATCATTCAAGTGGGCAATCGAATTA
  AATTTGTTATTAAAAGACCAGAACTTCTGACTCACAGTACCACTGAAGTTACTCAGCCAAGAACGAATAC
  ACCAGTCAAAGAAGATTGGAATGTCAGAATTACCAAGCTACGGAAGCAAGTGGAAGAGATTTTTAATTTG
  AAATTTGCTCAAGCTCTTGGACTCACCGAGGCAGTAAAAGTACCATATCCTGTGTTTGAATCAAACCCGG
  AGTTCTTGTATGTGGAAGGCTTGCCAGAGGGGATTCCCTTCCGAAGCCCTACCTGGTTTGGAATTCCACG
  ACTTGAAAGGATCGTCCGCGGGAGTAATAAAATCAAGTTCGTTGTTAAAAAACCTGAACTAGTTATTTCC
  TACTTGCCTCCTGGGATGGCTAGTAAAATAAACACTAAAGCTTTGCAGTCCCCCAAAAGACCACGAAGTC
  CTGGGAGTAATTCAAAGGTTCCTGAAATTGAGGTCACCGTGGAAGGCCCTAATAACAACAATCCTCAAAC
  CTCAGCTGTTCGAACCCCGACCCAGACTAACGGTTCTAACGTTCCCTTCAAGCCACGAGGGAGAGAGTTT
  TCCTTTGAGGCCTGGAATGCCAAAATCACGGACCTAAAACAGAAAGTTGAAAATCTCTTCAATGAGAAAT
  GTGGGGAAGCTCTTGGCCTTAAACAAGCTGTGAAGGTGCCGTTCGCGTTATTTGAGTCTTTCCCGGAAGA
  CTTTTATGTGGAAGGCTTACCTGAGGGTGTGCCATTCCGAAGACCATCGACTTTTGGCATTCCGAGGCTG
  GAGAAGATACTCAGAAACAAAGCCAAAATTAAGTTCATCATTAAAAAGCCCGAAATGTTTGAGACGGCGA
  TTAAGGAGAGCACCTCCTCTAAGAGCCCTCCCAGAAAAATAAATTCATCACCCAATGTTAATACTACTGC
  ATCAGGTGTTGAAGACCTTAACATCATTCAGGTGACAATTCCAGATGATGATAATGAAAGACTCTCGAAA
  GTTGAAAAAGCTAGACAGCTAAGAGAACAAGTGAATGACCTCTTTAGTCGGAAATTTGGTGAAGCTATTG
  GTATGGGTTTTCCTGTGAAAGTTCCCTACAGGAAAATCACAATTAACCCTGGCTGTGTGGTAGTTGATGG
  CATGCCCCCGGGGGTGTCCTTCAAAGCCCCCAGCTACCTGGAAATCAGCTCCATGAGAAGGATCTTAGAC
  TCTGCCGAGTTTATCAAATTCACGGTCATTAGACCATTTCCAGGACTTGTGATTAATAACCAGCTGGTTG
  ATCAGAGTGAGTCAGAAGGCCCCGTGATACAAGAATCAGCTGAACCAAGCCAGTTGGAAGTTCCAGCCAC
  AGAAGAAATAAAAGAGACTGATGGAAGCTCTCAGATCAAGCAAGAACCAGACCCCACGTGGTAG
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_033001 unedited
  CCTGGATTTTGTAAATCCGCACTTCACTATAGGGCGGACCGCGCAATTCGGCACGAGGCT
  CTCCAGCCGCGGCCGCCCGCCTGCCGCGGTCACCCCGGCCTCTGCCTCTGTCCCCCAGTG
  ATCGGATCAAGGCGCTGAGCGAGGCCCTGCCTGCGGGGCGGCCATGCGGCGGTGACAGGA
  GCGCGACCGACACGCACGGGCCCCTCGCCCCCTCTCGCCTCTTGTCCGCTCGCCAGCTCC
  CCTCATCCGAGGCTGCTCCGCGGCGGCCGCAGCCCGCGCGCGGCCCACACTCGCCTCCCC
  TCGGCACCCCCGGCCCCGGAGCTGCCTGGAGGCGGCCGCACTCGGGGATCATGGCCCAAG
  TTGCAATGTCCACCCTCCCCGTTGAAGATGAGGAGTCCTCGGAGAGCAGGATGGTGGTGA
  CATTCCTCATGTCAGCTCTCGAGTCCATGTGTAAAGAACTGGCCAAGTCCAAAGCCGAAG
  TGGCCTGCATTGCAGTGTATGAAACAGACGTGTTTGTCGTCGGAACTGATAGAGGACGTG
  CTTTTGTCAATACCAGATAGGATTTTCAAAAAGATTTTGTAAAATATTGTGTTGAAGAAG
  AAGATAAAGCTGCAGAGATGCATAAAATGAAATCTACAACCCATGCAAATCGGATGAGTG
  TAGATGCTGTAGATATTGANACACTCAGAANAACAGTTGAGGACTATTTCTGCTTTTGCT
  ATGGGAAATGCTTAGGCAATCCACAGTGGTACCTGTACCATATGATAAGATGCTGCGAGA
  CCAGTCAGCTGTGGTAGTGCATGGGCTTCCAGAATGTGTTGCCTTTTAACACCCCGAGTA
  CTATGATNNCTGCACCCCTGAATGGATCTTGGAGGACAAGCAGGGATTTCATTCATCATA
  AGAGACTTTTTTAGAGCCAAAAANNCATGTAGTGGTCNTGTGATGGTAACAGAGCTGACA
  GGTCN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_033001 unedited
  CCCCCCCTCCCNNTNNNTNNNTCACCATCCGTANCCCTNACTTGACTCTGGAACCGCGGC
  CGGCAACTAGNGATCGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTAGGGGGGGAA
  AGGCATGGATGGATTTTATTGATTACCCTATATCTACAATTTGAGGTAAAATAGAAGCAA
  CACATAAAAGGGCCTATTTCTGCTACCATGTCATATAATTCTCCATTGGGAATATTGGGA
  TAAAGCTACTGAAAACTATGCCGTCACAAAGCCTAGCTTTTTGTAAAGCTGGTATTTTAC
  AACTCGCATTGTTTGTGAAATCTCAACACATGTAAAACTCTCCTAGGAAGGCGCAAAACG
  TCAAAGGTTGCATCCTTAGCCCCCTGACCCCTCCTCACTCCCCGGGCTGGCACCTCAGGG
  TTACAAGAAAAACTAGGAAATAATGCCGGCCACGGGGACCCCTGGAGAGGGGGCCGGTTT
  AAAACAGCGTTCCTAAGAATCCGCGCCACAGCAGGTCCCGTGATGTTGGGGCCTTAGTGC
  CATTGACCTATCCCCAATCCTTAACCCGATTTTCAACTTCTGGCACACCATTGCATTTTA
  TTTTGGACGAAAAGTAAAAGTGGAAAAAGGTTTTCTTCTGGATTTCCCTTTTATAATTTA
  CACCCCTGAAAAAACTTGAACCTCCTGACTGTTAAGTCGGACTCCAAATGGTTTTTGGCT
  TCTAATTTATCGACAACAAAACCATTGGGAACCCATGAAAAAATATCTTTAGGCAAAAGG
  TTTTAATCCAAATAATACACCATCAGGAAACTTGGTTACTTCACCCCATTTAAGGGAGAA
  CCCCGGGAAACACAGGAATCATTTAAAAGGATTTTTTTTTTTTTACAATAGAAAAACCTA
  TAGAACCGCACCTCTTCCCAGGAAATTNTTTCTTTATTTGTAAAAAACTGTAATATTTCT
  CACTAAGTAAAAACAAGGAGAAATCCTTCCCTCTGCTCGGCCAACAC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_033001
• Insert Size: 4880 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_033001.1, NP_127494.1
• RefSeq Size: 4517 bp
• RefSeq ORF: 2934 bp
• Locus ID: 2969
• UniProt ID: P78347
• Cytogenetics: 7q11.23
• Domains: GTF2I
• Protein Families: Transcription Factors
• Protein Pathways: Basal transcription factors
• Gene Summary: This gene encodes a phosphoprotein containing six characteristic repeat motifs. The encoded protein binds to the initiator element (Inr) and E-box element in promoters and functions as a regulator of transcription. This locus, along with several other neighboring genes, is deleted in Williams-Beuren syndrome. There are many closely related genes and pseudogenes for this gene on chromosome 7. This gene also has pseudogenes on chromosomes 9, 13, and 21. Alternatively spliced transcript variants encoding multiple isoforms have been observed. [provided by RefSeq, Jul 2013]
  Transcript Variant: This variant (3) lacks an alternate in-frame exon, compared to variant 1. The encoded isoform (3, also known as alpha) is shorter than isoform 1.