beta 1 Adrenergic Receptor (ADRB1) (NM_000684) Human Untagged Clone

CAT#: SC127880

ADRB1 (untagged)-Human adrenergic, beta-1-, receptor (ADRB1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: beta 1 Adrenergic Receptor
• Synonyms: ADRB1R; B1AR; BETA1AR; FNSS2; RHR
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >SC127880 representing NM_000684.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  ATGGGCGCGGGGGTGCTTGTCCTGGGCGCCTCCGAGCCCGGTAACCTGTCGTCGGCCGCACCGCTCCCC
  GACGGCGCGGCCACCGCGGCGCGGCTGCTGGTGCCCGCGTCGCCGCCCGCCTCGTTGCTGCCTCCCGCC
  AGCGAAAGCCCCGAGCCGCTGTCTCAGCAGTGGACAGCGGGCATGGGTCTGCTGATGGCGCTCATCGTG
  CTGCTCATCGTGGCGGGCAATGTGCTGGTGATCGTGGCCATCGCCAAGACGCCGCGGCTGCAGACGCTC
  ACCAACCTCTTCATCATGTCCCTGGCCAGCGCCGACCTGGTCATGGGGCTGCTGGTGGTGCCGTTCGGG
  GCCACCATCGTGGTGTGGGGCCGCTGGGAGTACGGCTCCTTCTTCTGCGAGCTGTGGACCTCAGTGGAC
  GTGCTGTGCGTGACGGCCAGCATCGAGACCCTGTGTGTCATTGCCCTGGACCGCTACCTCGCCATCACC
  TCGCCCTTCCGCTACCAGAGCCTGCTGACGCGCGCGCGGGCGCGGGGCCTCGTGTGCACCGTGTGGGCC
  ATCTCGGCCCTGGTGTCCTTCCTGCCCATCCTCATGCACTGGTGGCGGGCGGAGAGCGACGAGGCGCGC
  CGCTGCTACAACGACCCCAAGTGCTGCGACTTCGTCACCAACCGGGCCTACGCCATCGCCTCGTCCGTA
  GTCTCCTTCTACGTGCCCCTGTGCATCATGGCCTTCGTGTACCTGCGGGTGTTCCGCGAGGCCCAGAAG
  CAGGTGAAGAAGATCGACAGCTGCGAGCGCCGTTTCCTCGGCGGCCCAGCGCGGCCGCCCTCGCCCTCG
  CCCTCGCCCGTCCCCGCGCCCGCGCCGCCGCCCGGACCCCCGCGCCCCGCCGCCGCCGCCGCCACCGCC
  CCGCTGGCCAACGGGCGTGCGGGTAAGCGGCGGCCCTCGCGCCTCGTGGCCCTGCGCGAGCAGAAGGCG
  CTCAAGACGCTGGGCATCATCATGGGCGTCTTCACGCTCTGCTGGCTGCCCTTCTTCCTGGCCAACGTG
  GTGAAGGCCTTCCACCGCGAGCTGGTGCCCGACCGCCTCTTCGTCTTCTTCAACTGGCTGGGCTACGCC
  AACTCGGCCTTCAACCCCATCATCTACTGCCGCAGCCCCGACTTCCGCAAGGCCTTCCAGGGACTGCTC
  TGCTGCGCGCGCAGGGCTGCCCGCCGGCGCCACGCGACCCACGGAGACCGGCCGCGCGCCTCGGGCTGT
  CTGGCCCGGCCCGGACCCCCGCCATCGCCCGGGGCCGCCTCGGACGACGACGACGACGATGTCGTCGGG
  GCCACGCCGCCCGCGCGCCTGCTGGAGCCCTGGGCCGGCTGCAACGGCGGGGCGGCGGCGGACAGCGAC
  TCGAGCCTGGACGAGCCGTGCCGCCCCGGCTTCGCCTCGGAATCCAAGGTGTAG
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000684 unedited
  ATAGGGCGGCCGCGAATTCGCCCTTCTGGGGTGTTCCCCAACCACGGCGNANCGCCGTAA
  CACCCCCCGCCCCCGGCCTCCGCAGCTCGGCATGGGCGCGGGGGTGCTTGTCCTGGGCGC
  CTCCGAGCCCGGTAACCTGTCGTCGGCCGCACCGCTCCCCGACGGCGCGGCCACCGCGGC
  GCGGCTGCTGGTGCCCGCGTCGCCGCCCGCCTCGTTGCTGCCTCCCGCCAGCGAAAGCCC
  CGAGCCGCTGTCTCAGCAGTGGACAGCGGGCATGGGTCTGCTGATGGCGCTCATCGTGCT
  GCTTATCGTGGCGGGCAATGTGCTGGTGATCGTGGCCCATCGCCAAGACGCCGCGGCTGG
  AGACGCTTAACAACCTTTTCATCATGTCCCTGGCCAGCGGCCACCTGGATAAGGGGCTAG
  TGGTGGTTGCCGTTCGGGGCAACATTCCGGTGTGGGGCCCACTGGGAAATCAGGTCCCTT
  TCTTTGCGAGCTTGGGACCTTAATGGAATTGCTTGTCGTGAAGGCCAGCCCCTCAACCCC
  GGGGGGTCTATGCCTGGACCATATATCTGGAACTAATTTGCGATTTTGCATCTAAAACAC
  GATAACTCCCCCCGGGGCACGGAGATCTCTTTGGTCCCAGTGTGGTCACACTACGGCTCT
  CGGATTCCTTCCTAACCATTTCACTAAGCCGTGGGGCAGGAGAATTAAGAAATAGCTGCA
  CGCTTGCTAAAGATCCCTGAAAGGTGGAGATGTGTCAATACTTGGTAACAT
  
• Restriction Sites: EcoRI-EcoRI
• ACCN: NM_000684
• Insert Size: 1434 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000684.1
• RefSeq Size: 1723 bp
• RefSeq ORF: 1434 bp
• Locus ID: 153
• UniProt ID: P08588
• Cytogenetics: 10q25.3
• Protein Families: Druggable Genome, GPCR, Transmembrane
• Protein Pathways: Calcium signaling pathway, Dilated cardiomyopathy, Endocytosis, Gap junction, Neuroactive ligand-receptor interaction
• MW: 51.2 kDa
• Gene Summary: The adrenergic receptors (subtypes alpha 1, alpha 2, beta 1, and beta 2) are a prototypic family of guanine nucleotide binding regulatory protein-coupled receptors that mediate the physiological effects of the hormone epinephrine and the neurotransmitter norepinephrine. Beta-1 adrenoceptors are predominately located in the heart. Specific polymorphisms in this gene have been shown to affect the resting heart rate and can be involved in heart failure. [provided by RefSeq, Sep 2019]