C19orf12 (NM_031448) Human Untagged Clone

CAT#: SC127755

C19orf12 (untagged)-Human chromosome 19 open reading frame 12 (C19orf12), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: C19orf12
• Synonyms: MPAN; NBIA3; NBIA4; SPG43
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_031448, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGACTATCATGGTGGAGGACATCATGAAGCTGCTGTGCTCCCTTTCTGGGGAGAGGAAGATGAAGGCGG
  CTGTCAAGCACTCTGGGAAGGGTGCCCTGGTCACAGGGGCCATGGCCTTCGTCGGGGGTTTGGTGGGCGG
  CCCACCGGGACTCGCCGTTGGGGGGGCTGTCGGGGGGCTGTTAGGTGCCTGGATGACAAGTGGACAGTTT
  AAGCCGGTTCCTCAGATCCTAATGGAGCTGCCCCCTGCCGAGCAACAGAGGCTCTTTAACGAAGCCGCAG
  CCATCATCAGGCACCTGGAGTGGACGGACGCCGTGCAGCTGACCGCGCTGGTCATGGGCAGCGAGGCCCT
  GCAGCAGCAGCTGCTGGCCATGCTGGTGAACTACGTCACCAAGGAGCTGCGGGCCGAGATCCAGTATGAT
  GACTAG
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_031448 unedited
  CCGCGAATTCGGCACGAGCCGGGCTGCAGCCGCGTCTGATCGCCGAGCGCGCCGCGTAGA
  CCTCCGCTCCCCCAGGCCCGCCACGATGACTATCATGGTGGAGGACATCATGAAGCTGCT
  GTGCTCCCTTTCTGGGGAGAGGAAGATGAAGGCGGCTGTCAAGCACTCTGGGAAGGGTGC
  CCTGGTCACAGGGGCCATGGCCTTCGTCGGGGGTTTGGTGGGCGGCCCACCGGGACTCGC
  CGTTGGATCCAGTCTAAGACACCGCATTGCATTGAATCGCCTGCTTTCTTAGTCTCCTCT
  GGCCTTTAACACTTTCTCAGACTTGCCTTGTTCACAGCCTTGATAGTTTTGAGGAGTACT
  GGGCAGGGGGGGCTGTCGGGGGGCTGTTAGGTGCCTGGATGACAAGTGGACAGTTTAAGC
  CGGTTCCTCAGATCCTAATGGAGCTGCCCCCTGCCGAGCAACAGAGGCTCTTTAACGAAG
  CCGCAGCCATCATCAGGCACCTGGAGTGGACGGACGCCGTGCAGCTGACCGCGCTGGTCA
  TGGGCAGCGAGGCCCTGCAGCAGCAGCTGCTGGCCATGCTGGTGAACTACGTCACCAAGG
  AGCTGCGGGCCGAGATCCAGTATGATGACTANNGCCGCACCTCGGNGAGGTGGGNGGGCC
  CCTTTAATGACTCTGTGATTCTGAGAGGTGCTTGGGAGTTGGAGAGCCCCACGATGCCCC
  TGGGGATCTCACATCATCAGTGTTTAT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_031448
• Insert Size: 3670 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_031448.2, NP_113636.1
• RefSeq Size: 4683 bp
• RefSeq ORF: 4683 bp
• Locus ID: 83636
• UniProt ID: Q9NSK7
• Cytogenetics: 19q12
• Protein Families: Transmembrane
• Gene Summary: This gene encodes a small transmembrane protein. Mutations in this gene are a cause of neurodegeneration with brain iron accumulation-4 (NBIA4), but the specific function of the encoded protein is unknown. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Dec 2011]
  Transcript Variant: This variant (2) differs in the 5' UTR and initiates translation at a downstream, in-frame start codon, compared to variant 1. Variants 2 and 4 encode the same isoform (2), which has a shorter N-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.