EAAT2 (SLC1A2) (AK090810) Human Untagged Clone

CAT#: SC127753

(untagged)-Human cDNA FLJ33491 fis, clone BRAMY2003653

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: EAAT2
• Synonyms: EAAT2; GLT-1; HBGT
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for AK090810, the custom clone sequence may differ by one or more nucleotides
  
  
  AAAGTGCTGGGATTACAGGCGTGAGCCACCGTGCCCGGCCAGAAAATACATTCTTTACAGCAAATGTTAT
  AGGTTAAGGAGACAGTTCCTGGGTTTAGCTGTGAAACTGTTTAGGATTTTCTTTAAGTGGTGTTCCCAAG
  AGCTTTTTTCTGGTAAGTTGGAGGCTATAGGCAAAGCGTCTAGGAGCTTGTTGTTTTTTGCTATGGAAGG
  CCAGCTTTGGGATCTATATACATGGGCATGTTTCTCAATGGAGTGGTCACAGGATTCTCTTCTCAGAACT
  CTGCCTGAAACCTTTCCGGTGTGTTTTGTCTTCAACTTTTTCCTAGCTATCCTATTAGCCTGTAAATATG
  TCAGCAGACAATGTGTTTGGAAGAAACAAGGTGTACAACTTCACAAAGCAAGTTTTTTCTGGTCTGAGAC
  TTACTGTGTGCAGGAAGTCTCGTGAGAATATACTTAGAAAATCACACTTATCTGGAAATTCAATAAGTCA
  TATTAAAAATATACAATTTCTTGAACTCAGGACTAGCCTGAAAATCCAAAATGCATGTAGAGTTGACATG
  TTGACATATGTCTATGTCCTCTGTTTTTATGTAAGCCTCATTTATTTCTCCCTTGCCATGCCCATTTGGG
  ATTTTCTTTGCCCAGCATATGTGCCATAAACAGAACAAATGGATCCAGGGTATACCTCTAATAACCTTTA
  TCTAAATAGAATACATTTAACCTGTCAATTATATGTATAGATTTATCCTAATTGGTTCAACATATATATG
  AATGCTTGTAATAGTCTGTTACTATTTGATAATGCATAAATGTTATATTTATATACAGTTACAGCAGGCC
  AGAAACATACATACACTGCATCCTGTAAGGTCTGTAAATCAGGGCTTCGGCAATGCCATTTTGCTATAGA
  CTCATTCATGTTTTTTAGGAATCAGAATCATTTCAGGAGAAAACCAACTAAGTAGACTTTAATTCTTCTC
  TTTCTCATTCTTGCCCTTTGAGAAACCCAAGTGGAATCATCACTTAATTACTTACTCAAATTCAATGAAT
  CAAATCCAATCCAATCAGTGAGAAGAATAGCTTATCTTTCAAGGTAATACTTTACCTTAGATCTTTGAGG
  GCATGCTTCCCATACAAAGAGCATCAGAAAATCAGGGCAATCATCTAGATCTCACAGATCTTCAACATAG
  GACTCTAGGTTATGAGAGCTGAACCACATAGCTATCAGTTTCAGTTTTTACTTCCTATTGAGTGTGTAGA
  AACACAGCCTATGGTTTTGCTGTCTGCAGAAGTTACCATACCCTGTAAAAGACAAATATTTCTTTCTCCT
  CTCGAACCACTAGTCAGCCTGGATATTCATGAAAGCTGAAAGAAGGGGTGCCAAAATGCCTAGCTCCTGG
  ATTTAGACTCATGGAATATGAGAAAGGCTTAGGGAAAATTCAAGTCATGGGGAGATTCCTGCTAGGTGAT
  GCCAGGAATCTCAATGACCTGTTGATTAGAGGGTCTCTGGTCATTTCCACTTGTGTGTGGAGAAATACAT
  CATTTGCATATGTATCTAGTCAAGAAGCCCTTTTGGAGCAAAGTGGTTGGGAAACTCATTTACTGGGTCC
  ACCAAAGGAAACCCAATTCTGCCATGTTTTTGCAAATACTACACGTCCCTTTGCTCTCAATAGCAAAAGT
  GATGTTATAGTTTTTTTTACTTTTGTCAGAATCAAGGCTTCGGCCACCTGTGCAATATTTTCCCATGGAA
  TGCAGCAGTAAATCCAAGTAGGTATATCCGTGCTCAGAGAGAATTCTCCATAGTTCTGACTCCTGAAAAA
  CAAGTCTCATTAGCTCTCATCTAGTTTGATGACTAAAAGTGCACAGCAGTTATACCCCAGTGGAAGTGAT
  TTTTCTCACCTAGCTGCTCTCTATCTCTTACGGCTATCTAGTTGTCTGAGCTGCTGGTTTGTTTTACTTA
  TTCTCCAACTGAACTCATTCCAAACCAAGAAAAAGGACCAAATGAAATTTTCAGTTATTATCATACTTGC
  TCCTTGAATAAGAATGCTAGAGTCTGGAGGCAGAAGAAGACTAAGTT
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for AK090810 unedited
  TTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGCACGAGAAAAGCCTAGCTCCTG
  GATTTTGGCACCCCTTCTTTCAGCTTTCATGAATATCCAGGCTGACTAGTGGTTCGAGAG
  GAGAAAGAAAGGGTGCCAAAATGCCTAGCTCCTGGATTTAGACTCATGGAATATGAGAAA
  GGCTTAGGGAAAATTCAAGTCATGGGGAGATTCCTGCTAGGTGATGCCAGGAATCTCAAT
  GACCTGTTGATTAGAGGGTCTCTGGTCATTTCCACTTGTGTGTGGAGAAATACATCATTT
  GCATATGTATCTAGTCAAGAAGCCCTTTTGGAGCAAAGTGGTTGGGAAACTCATTTACTG
  GGTCCACCAAAGGAAACCCAATTCTGCCATGTTTTTGCAAATACTACACGTCCCTTTGCT
  CTCAATAGCAAAAGTGATGTTATAGTTTTTTTTACTTTTGTCAGAATCAAGGCTTCGGCC
  ACCTGTGCAATATTTTCCCATGGAATGCAGCAGTAAATCCAAGTAGGTATATCCCGTGCT
  CAGAGAGAATTCTCCATAGTTCTGACTCCTGAAAAACAAGTCTCATTAGCTCTCATCTAG
  TTTGATGACTAAAAGTGCACAGCAGTTATACCCCAGTGGAAGTGATTTTTCTCACCTAGC
  TGCTCTCTAACTCTTACGGCTATCTAGTTGTCTGAGCTGCTGGTTTGTTTTACTTATTCT
  NCAAACTGACTCATTCCANACCAAGAAAAAAGACCAATGAAATNNTCAGTTTTATCATAC
  TTGCTCCTTNNGATAAATGCTAGAGTCTGGAGCAGAAAAGACTAGTTAANAAAAACACCT
  GAAGATTCTGNAGTGAGTTTTGTAGTCTAATGCAAGAGAAAATC
  
• Restriction Sites: NotI-NotI
• ACCN: AK090810
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: AK090810.1
• RefSeq Size: 2077 bp
• RefSeq ORF: 2077 bp
• Locus ID: 6506
• Cytogenetics: 11p13
• Protein Families: Transmembrane
• Protein Pathways: Amyotrophic lateral sclerosis (ALS)
• Gene Summary: This gene encodes a member of a family of solute transporter proteins. The membrane-bound protein is the principal transporter that clears the excitatory neurotransmitter glutamate from the extracellular space at synapses in the central nervous system. Glutamate clearance is necessary for proper synaptic activation and to prevent neuronal damage from excessive activation of glutamate receptors. Improper regulation of this gene is thought to be associated with several neurological disorders. Alternatively spliced transcript variants of this gene have been identified. [provided by RefSeq, Jun 2017]