TSC22D3 (NM_004089) Human Untagged Clone

CAT#: SC127719

TSC22D3 (untagged)-Human TSC22 domain family, member 3 (TSC22D3), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TSC22D3
• Synonyms: DIP; DSIPI; GILZ; TSC-22R
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC127719 sequence for NM_004089 edited (data generated by NextGen Sequencing)
  ATGAACACCGAAATGTATCAGACCCCCATGGAGGTGGCGGTCTACCAGCTGCACAATTTC
  TCCATCTCCTTCTTCTCTTCTCTGCTTGGAGGGGATGTGGTTTCCGTTAAGCTGGACAAC
  AGTGCCTCCGGAGCCAGCGTGGTGGCCATAGACAACAAGATCGAACAGGCCATGGATCTG
  GTGAAGAATCATCTGATGTATGCTGTGAGAGAGGAGGTGGAGATCCTGAAGGAGCAGATC
  CGAGAGCTGGTGGAGAAGAACTCCCAGCTAGAGCGTGAGAACACCCTGTTGAAGACCCTG
  GCAAGCCCAGAGCAGCTGGAGAAGTTCCAGTCCTGTCTGAGCCCTGAAGAGCCAGCTCCC
  GAATCCCCACAAGTGCCCGAGGCCCCTGGTGGTTCTGCGGTGTAA
  
  Clone variation with respect to NM_004089.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004089 unedited
  TGGCTTTGGGTTCGTTCTGCAGCCTTCGCCCCGCTCCTAGCCTCAGGGCCGGACTCCAGC
  GCAGAGCCCAGCCCAGCGCAGCCTGCCAGCAGCCACCCATCCGCCCAGCCGCCCAGCCCC
  GCACGAAACCCGGCCAGAGCTTCCTAGCAGCCCGAGCCATGAACACCGAAATGTATCAGA
  CCCCCATGGAGGTGGCGGTCTACCAGCTGCACAATTTCTCCATCTCCTTCTTCTCTTCTC
  TGCTTGGAGGGGATGTGGTTTCCGTTAAGCTGGACAACAGTGCCTCCGGAGCCAGCGTGG
  TGGCCATAGACAACAAGATCGAACAGGCCATGGATCTGGTGAAGAATCATCTGATGTATG
  CTGTGAGAGAGGAGGTGGAGATCCTGAAGGAGCAGATCCGAGAGCTGGTGGAGAAGAACT
  CCCAGCTAGAGCGTGAGAACACCCTGTTGAAGACCCTGGCAAGCCCAGAGCAGCTGGAGA
  AGTTCCAGTCCTGTCTGAGCCCTGAAGAGCCAGCTCCCGAATCCCCACAAGTGCCCGAGG
  CCCCTGGTGGTTCTGCGGTGTAAGTGGCTCTGTCCTCAGGGTGGGCAGAGCCACTAAACT
  TGTTTTACTAGTTCTTTCCAGTTTGGTTTTGGCTTCCCCAGCATCATCTCACGAGGAGAA
  CTTTACACCTAGCACAGCTGGTGCCAAGAAGAGTCCTAAAGACCTGGGCCACNTGGGTCC
  ACTCCAGCGACAGACCCCTGACAGAGCANGTCTCTGGAGGCTGAGNTGCATGGGGCCTAN
  NTACCCCAGCCAGGGAGCCTTTATGCTACTGCGC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_004089
• Insert Size: 2000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004089.3, NP_004080.2
• RefSeq Size: 1986 bp
• RefSeq ORF: 405 bp
• Locus ID: 1831
• UniProt ID: Q99576
• Cytogenetics: Xq22.3
• Domains: TSC22
• Protein Families: Transcription Factors
• Gene Summary: This gene encodes the anti-inflammatory protein glucocorticoid (GC)-induced leucine zipper. Expression of this gene stimulated by glucocorticoids and interleukin 10 and it appears to play a key role in the anti-inflammatory and immunosuppressive effects of this steroid. This protein has also been shown to inhibit pro-inflammatory molecules including nuclear factor κB. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jan 2016]
  Transcript Variant: This variant (2) has a different 5' end exon compared to transcript variant 1, resulting in an isoform (2) with a different and a shorter N-terminus compared to isoform 1.