ATP6V1E1 (NM_001696) Human Untagged Clone

CAT#: SC127436

ATP6V1E1 (untagged)-Human ATPase, H+ transporting, lysosomal 31kDa, V1 subunit E1 (ATP6V1E1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ATP6V1E1
• Synonyms: ARCL2C; ATP6E; ATP6E2; ATP6V1E; P31; Vma4
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC127436 sequence for NM_001696 edited (data generated by NextGen Sequencing)
  ATGGCTCTCAGCGATGCTGACGTGCAAAAGCAGATAAAGCATATGATGGCTTTCATTGAA
  CAAGAAGCCAATGAGAAAGCAGAAGAAATAGATGCAAAGGCAGAAGAAGAGTTCAACATA
  GAGAAAGGTCGGCTTGTGCAAACCCAAAGACTAAAGATTATGGAATATTATGAGAAGAAA
  GAGAAACAGATTGAGCAGCAGAAGAAAATTCAGATGTCCAATTTGATGAATCAAGCGAGA
  CTCAAAGTCCTCAGAGCAAGAGATGACCTTATCACAGACCTACTAAATGAAGCAAAACAG
  AGACTCAGCAAGGTGGTAAAAGATACAACCAGGTACCAAGTGCTGCTGGATGGACTGGTT
  CTCCAGGGTTTGTACCAGTTGCTGGAGCCCCGAATGATTGTTCGTTGCAGGAAACAAGAT
  TTCCCTCTGGTAAAGGCTGCAGTGCAGAAGGCAATTCCTATGTACAAAATTGCCACCAAA
  AACGATGTTGATGTCCAAATTGACCAGGAGTCCTACCTGCCTGAAGACATAGCTGGTGGA
  GTTGAGATCTATAATGGAGATCGTAAAATAAAGGTTTCCAACACCCTGGAAAGCCGGCTG
  GATCTCATAGCCCAGCAGATGATGCCAGAAGTCCGGGGAGCCTTGTTTGGTGCAAATGCC
  AACAGGAAGTTTTTGGACTAA
  
  Clone variation with respect to NM_001696.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001696 unedited
  TTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGGAACCGGAACCCG
  CGGTTATAAAGTAAAGGAACCCGAGATCTGCGCAGGGGTTCCCTTTGCCGATTTCTCTCA
  CCTCACCTTTCAAACCTAAACTCGAGCCTGCTGTTCACCGGCCTAGCATTGCTCTCGCCA
  TGGCTCTCAGCGATGCTGACGTGCAAAAGCAGATAAAGCATATGATGGCTTTCATTGAAC
  AAGAAGCCAATGAGAAAGCAGAAGAAATAGATGCAAAGGCAGAAGAAGAGTTCAACATAG
  AGAAAGGTCGGCTTGTGCAAACCCAAAGACTAAAGATTATGGAATATTATGAGAAGAAAG
  AGAAACAGATTGAGCAGCAGAAGAAAATTCAGATGTCCAATTTGATGAATCAAGCGAGAC
  TCAAAGTCCTCAGAGCAAGAGATGACCTTATCACAGACCTACTAAATGAAGCAAAACAGA
  GACTCAGCAAGGTGGTAAAAGATACAACCAGGTACCAAGTGCTGCTGGATGGACTGGTTC
  TCCAGGGTTTGTACCAGTTGCTGGAGCCCCGAATGATTGTTCGTTGCAGGAAACAAGATT
  TCCCTCTGGTAAAGGCTGCAGTGCAGAAGGCAATTCCTATGTACAAAATTGCCACCAAAA
  ACGATGTTGATGTCCAAATTGACCAGGAGTCCTACCTGCCTGAAGCATAGCTGTTGGAGT
  TGAGATCTATAATGGAGATCGTANAATAAGGTTTCCAACACCCTGGAAAGCCGCTGGATC
  TCATAGCCCAGCAGATGATGCCAGAAGTCCGGNGAGCCCTGTTTGGTGCNATGCCACAGG
  AAGTTTTGGNACTAAGCCTTCAGAGTGGAGCTCGTCGCAGCTCTCTGCTGTGAGTGGAAG
  CN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001696 unedited
  TNCTGGNCTTAGNATTTAAATCACATTTACAGATGAAGGGAAAACTTCTAGGCCTAAACA
  GGTAACAGTAAAGCGACTACTGCTGGAAGGAGTGGTGTGCCACATGCGCCTTAAGCCTCA
  AGAGTGGGGACTGCAGGGCCAAACACCAAATACATCACCTTTAGGCCAGACGGAGAGTGG
  AGACCCAGGAAGCCCATGCAACCACCATCTGCCCCCTCCCCTAGTGCTGCAGAACCGGCT
  GGACACTGTCACACTTTCAGAAGAACTGGAGGTGGGTCCTGATCATATTACATGAAGCCT
  TCCACCATTGTGAACAATTAGGCAAGGCATGAGTGACAGAGGGGCATCGCTGATAAATAC
  AGAGCAATACTGGGGCAGTGAAGAGGAAGCTACAGAGACATTCGTGTTTCTTCAAATATC
  AGAAGCTTCCACATCACAGCAGGAGAGCTGACGACGAGCTCCACCTCCTGAAGGCTTAGT
  CCAAAAACTTCCTGTTGGCATTTGCACCAAACAAGGCTCCCCGGACTTCTGGCATCATCT
  GCTGGGCTATGAGATCCAGCCGGCTTTCCAGGGTGTTGGAAACCTTTATTTTACGATCTC
  CATTATAGATCTCAACTCCACCAGCTATGTCTTCAGGCAGGTAGGACTCCTGGTCAATTT
  GGACATCAACATCGTTTTTGGTGGCAATTTTGTACATAGGAATTGCCTTCTGCACTGCAG
  CCTTTACCAGAGGGAAATCTTGTTTCCCTGCACGAACAATCATTCNGNGCTCCCAGCACT
  GGTACANACCCTGGAGAACCAGTCCATCCAGCAGCACTTGGTACCTGGNTGTATCTTTTA
  CACCTTG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001696
• Insert Size: 1400 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001696.2, NP_001687.1
• RefSeq Size: 1330 bp
• RefSeq ORF: 681 bp
• Locus ID: 529
• UniProt ID: P36543
• Cytogenetics: 22q11.21
• Domains: vATP-synt_E
• Protein Pathways: Epithelial cell signaling in Helicobacter pylori infection, Metabolic pathways, Oxidative phosphorylation, Vibrio cholerae infection
• Gene Summary: This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A, three B, and two G subunits, as well as a C, D, E, F, and H subunit. The V1 domain contains the ATP catalytic site. This gene encodes alternate transcriptional splice variants, encoding different V1 domain E subunit isoforms. Pseudogenes for this gene have been found in the genome. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (a).