MNDA (NM_002432) Human Untagged Clone

CAT#: SC127417

MNDA (untagged)-Human myeloid cell nuclear differentiation antigen (MNDA)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MNDA
• Synonyms: PYHIN3
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC127417 sequence for NM_002432 edited (data generated by NextGen Sequencing)
  ATGGTGAATGAATACAAGAAAATTCTTTTGCTGAAAGGATTTGAGCTCATGGATGATTAT
  CATTTTACATCAATTAAGTCCTTACTGGCCTATGATTTAGGACTAACTACAAAAATGCAA
  GAGGAATACAACAGAATTAAGATTACAGATTTGATGGAAAAAAAGTTCCAAGGCGTTGCC
  TGTCTAGACAAACTAATAGAACTTGCCAAAGATATGCCATCACTTAAAAACCTTGTTAAC
  AATCTTCGAAAAGAGAAGTCAAAAGTTGCTAAGAAAATTAAAACACAAGAAAAAGCTCCA
  GTGAAAAAAATAAACCAGGAAGAAGTGGGTCTTGCGGCACCTGCACCCACCGCAAGAAAC
  AAACTGACATCGGAAGCAAGAGGGAGGATTCCTGTAGCTCAGAAAAGAAAAACTCCAAAC
  AAAGAAAAGACTGAAGCCAAAAGGAATAAGGTGTCCCAAGAGCAGAGTAAGCCCCCAGGT
  CCCTCAGGAGCCAGCACATCTGCAGCTGTGGATCATCCCCCACTACCCCAGACCTCATCA
  TCAACTCCATCCAACACTTCGTTTACTCCGAATCAGGAAACCCAGGCCCAACGGCAGGTG
  GATGCAAGAAGAAATGTTCCCCAAAACGACCCAGTGACAGTGGTGGTACTGAAAGCAACA
  GCGCCATTTAAATACGAGTCCCCAGAAAATGGGAAAAGCACAATGTTTCATGCTACAGTG
  GCCAGTAAGACTCAATATTTCCATGTGAAAGTCTTCGACATCAACTTGAAAGAGAAATTT
  GTAAGGAAGAAGGTCATTACCATATCTGATTACTCTGAATGTAAAGGAGTAATGGAAATA
  AAGGAAGCATCATCTGTGTCTGACTTTAATCAAAATTTTGAGGTCCCAAACAGAATTATC
  GAAATAGCAAATAAAACTCCCAAGATCAGTCAACTTTACAAGCAAGCATCTGGAACAATG
  GTGTATGGGTTGTTTATGTTACAAAAGAAAAGCGTACACAAGAAGAACACAATTTATGAA
  ATACAGGATAATACAGGATCCATGGATGTAGTGGGGAGTGGAAAATGGCACAATATCAAG
  TGTGAGAAAGGAGATAAACTTCGACTCTTCTGCCTTCAACTGAGAACAGTTGACCGCAAG
  CTGAAACTGGTGTGTGGAAGTCACAGCTTCATCAAGGTCATCAAGGCCAAGAAAAACAAG
  GAAGGACCAATGAATGTTAATTGA
  
  Clone variation with respect to NM_002432.1
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002432 unedited
  GGTTAGAATTTGTATACGACTCCTATAGGGCGGCCGCGATTCGGCACGAGGATCAAGATT
  GAGAGTGGCTCTAACAAGTGCCATTTTTCCTTGTTAGCTTTCATTTCTCAGCCCTTTACA
  AGATTAAAATAGTCTGCAGTTTAATCTCTCCAAAGCTTTACGGACAGTGATTCTGTCCTA
  AACAAGACAGTGACTCCAGGATTTCTGAAGACTATTGTGGAAGAAGCATCCATTAAGGCC
  AAGCTATAACATCAGAAATGGTGAATGAATACAAGAAAATTCTTTTGCTGAAAGGATTTG
  AGCTCATGGATGATTATCATTTTACATCAATTAAGTCCTTACTGGCCTATGATTTAGGAC
  TAACTACAAAAATGCAAGAGGAATACAACAGAATTAAGATTACAGATTTGATGGAAAAAA
  AGTTCCAAGGCGTTGCCTGTCTAGACAAACTAATAGAACTTGCCAAAGATATGCCATCAC
  TTAAAAACCTTGTTAACAATCTTCGAAAAGAGAAGTCAAAAGTTGCTAAGAAAATTAAAA
  CACAAGAAAAAGCTCCAGTGAAAAAAATAAACCAGGAAGAAGTGGGTCTTGCGGCACCTG
  CACCCACCGCAAGAAACAAACTGACATCGGAAGCAAGAGGGAGGATTCCTGTAGCTCAGA
  AAAGAAAAACTCCAAACANAGAANAGACTGAAGCCAAAAGGAATAAGGTGTCCCAAGAGC
  AGAGTAAGCCCCCAGGTCCCTCAGGAGCCAGCACATCTGCAGCTGTGGATCATCCCCCAC
  TACCCCAGACCTCATCATCAACTCCATCCCACACTTCGTTTACTCCGGTACACTCTTCCT
  GGCCCTCTTCTCCATTTTTTTTTAACCCATCACAGTGCATTCCCCTGTTACTATNGGTAC
  TCTCATGCATACCTCT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002432 unedited
  AAAGATATTTTATTTTTTGCCAGTGATGTCATAAAGCAAATGTAATATCTAAAAAAAAGG
  AGAGTATAAGAAGAAATTTTATTCCAGAAAGCAAACTAATCTATAACTCAGTTATAAAAG
  CTCTTAATATGCTAGAATATATCTACTGAAACATCATTTAAATGAATTACTGAAATTTAC
  AAAATCACAGTTATTAACAACTTAATTGTTTTAAGCGGAAGTTGTTTGTTGCATTTCAGC
  TTTCATATTTCAATTAACATTCATTGGTCCTTCCTTGTTTTTCTTGGCCTTGATGAACCT
  TGATGAAGCTGTGACTTCCACACACCAGTTTCAGCTTGCGGTCAACTGTTCTCAGTTGAA
  AGCAGAAGAAGTCGAAGTTTATCTCCTTTCTCACACTTGATATTGTGCCATTTTCCACTC
  CCCACTACATCCATGGATCCTGTATTATCCTGTATTTCATAAATTGTGTTCTTCTTGTGT
  ACGCTTTTCTTTTGTAACATAAACAACCCATACACCATTGTTCCAGATGCTTGCTTGTAA
  AGTTGACTGATCTTGGGAGTTTTATTTGCTATTTCGATAATTCTGTTTGGGACCTCAAAA
  TTTTGATTAAAGTCAGACACAGATGANTGCTTCCTTTATTTCCATTACTCCTTTACATTC
  AGAGTAATCAGATATGGTAATGAACCTTCTTCCTTACAAATNTCTCTTTCAAGTTGATGT
  CGAAAGACTNTCACATGGNAAATATTGAGTCTTACTGGGCCACTGTAGCATGATACATTN
  NGTGCTNTTNCCATTTTCTGGGGGACTCGTATTAATTGGNNCGCTGNTGCTTTCAGGTAC
  ACCACTGTCACTGGGGGTCGTTTAGGGGAACAATTTCTNCTGCATCCACNCTGCCGTTAG
  CCNTGGAGTTNNCTGATTTCTGGGNTGGCGCAGAACATGAATATTAACTCTAAACTTTGA
  GCANGAAGGNGGGCCCGGTGGGCTTCACCCTGTATTTCTACACTTTGGGAAGNCNAGGGG
  GNTGGATACACAGGTCANAAAATACGAAACATCTCGCTAATAA
  
• Restriction Sites: ECoRI-NOT
• ACCN: NM_002432
• Insert Size: 1800 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002432.1, NP_002423.1
• RefSeq Size: 1670 bp
• RefSeq ORF: 1224 bp
• Locus ID: 4332
• UniProt ID: P41218
• Cytogenetics: 1q23.1
• Domains: PAAD_DAPIN, HIN
• Protein Families: Transcription Factors
• Gene Summary: The myeloid cell nuclear differentiation antigen (MNDA) is detected only in nuclei of cells of the granulocyte-monocyte lineage. A 200-amino acid region of human MNDA is strikingly similar to a region in the proteins encoded by a family of interferon-inducible mouse genes, designated Ifi-201, Ifi-202, and Ifi-203, that are not regulated in a cell- or tissue-specific fashion. The 1.8-kb MNDA mRNA, which contains an interferon-stimulated response element in the 5-prime untranslated region, was significantly upregulated in human monocytes exposed to interferon alpha. MNDA is located within 2,200 kb of FCER1A, APCS, CRP, and SPTA1. In its pattern of expression and/or regulation, MNDA resembles IFI16, suggesting that these genes participate in blood cell-specific responses to interferons. [provided by RefSeq, Jul 2008]