SEMA4B (NM_020210) Human Untagged Clone

CAT#: SC127234

SEMA4B (untagged)-Human sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4B (SEMA4B), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SEMA4B
• Synonyms: SEMAC; SemC
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_020210, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGCTGCGCACCGCGATGGGCCTGAGGAGCTGGCTCGCCGCCCCATGGGGCGCGCTGCCGCCTCGGCCAC
  CGCTGCTGCTGCTCCTGCTGCTGCTGCTCCTGCTGCAGCCGCCGCCTCCGACCTGGGCGCTCAGCCCCCG
  GATCAGCCTGCCTCTGGGCTCTGAAGAGCGGCCATTCCTCAGATTCGAAGCTGAACACATCTCCAACTAC
  ACAGCCCTTCTGCTGAGCAGGGATGGCAGGACCCTGTACGTGGGTGCTCGAGAGGCCCTCTTTGCACTCA
  GTAGCAACCTCAGCTTCCTGCCAGGCGGGGAGTACCAGGAGCTGCTTTGGGGTGCAGACGCAGAGAAGAA
  ACAGCAGTGCAGCTTCAAGGGCAAGGACCCACAGCGCGACTGTCAAAACTACATCAAGATCCTCCTGCCG
  CTCAGCGGCAGTCACCTGTTCACCTGTGGCACAGCAGCCTTCAGCCCCATGTGTACCTACATCAACATGG
  AGAACTTCACCCTGGCAAGGGACGAGAAGGGGAATGTCCTCCTGGAAGATGGCAAGGGCCGTTGTCCCTT
  CGACCCGAATTTCAAGTCCACTGCCCTGGTGGTTGATGGCGAGCTCTACACTGGAACAGTCAGCAGCTTC
  CAAGGGAATGACCCGGCCATCTCGCGGAGCCAAAGCCTTCGCCCCACCAAGACCGAGAGCTCCCTCAACT
  GGCTGCAAGACCCAGCTTTTGTGGCCTCAGCCTACATTCCTGAGAGCCTGGGCAGCTTGCAAGGCGATGA
  TGACAAGATCTACTTTTTCTTCAGCGAGACTGGCCAGGAATTTGAGTTCTTTGAGAACACCATTGTGTCC
  CGCATTGCCCGCATCTGCAAGGGCGATGAGGGTGGAGAGCGGGTGCTACAGCAGCGCTGGACCTCCTTCC
  TCAAGGCCCAGCTGCTGTGCTCACGGCCCGACGATGGCTTCCCCTTCAACGTGCTGCAGGATGTCTTCAC
  GCTGAGCCCCAGCCCCCAGGACTGGCGTGACACCCTTTTCTATGGGGTCTTCACTTCCCAGTGGCACAGG
  GGAACTACAGAAGGCTCTGCCGTCTGTGTCTTCACAATGAAGGATGTGCAGAGAGTCTTCAGCGGCCTCT
  ACAAGGAGGTGAACCGTGAGACACAGCAGTGGTACACCGTGACCCACCCGGTGCCCACACCCCGGCCTGG
  AGCGTGCATCACCAACAGTGCCCGGGAAAGGAAGATCAACTCATCCCTGCAGCTCCCAGACCGCGTGCTG
  AACTTCCTCAAGGACCACTTCCTGATGGACGGGCAGGTCCGAAGCCGCATGCTGCTGCTGCAGCCCCAGG
  CTCGCTACCAGCGCGTGGCTGTACACCGCGTCCCTGGCCTGCACCACACCTACGATGTCCTCTTCCTGGG
  CACTGGTGACGGCCGGCTCCACAAGGCAGTGAGCGTGGGCCCCCGGGTGCACATCATTGAGGAGCTGCAG
  ATCTTCTCATCGGGACAGCCCGTGCAGAATCTGCTCCTGGACACCCACAGGGGGCTGCTGTATGCGGCCT
  CACACTCGGGCGTAGTCCAGGTGCCCATGGCCAACTGCAGCCTGTACAGGAGCTGTGGGGACTGCCTCCT
  CGCCCGGGACCCCTACTGTGCTTGGAGCGGCTCCAGCTGCAAGCACGTCAGCCTCTACCAGCCTCAGCTG
  GCCACCAGGCCGTGGATCCAGGACATCGAGGGAGCCAGCGCCAAGGACCTTTGCAGCGCGTCTTCGGTTG
  TGTCCCCGTCTTTTGTACCAACAGGGGAGAAGCCATGTGAGCAAGTCCAGTTCCAGCCCAACACAGTGAA
  CACTTTGGCCTGCCCGCTCCTCTCCAACCTGGCGACCCGACTCTGGCTACGCAACGGGGCCCCCGTCAAT
  GCCTCGGCCTCCTGCCACGTGCTACCCACTGGGGACCTGCTGCTGGTGGGCACCCAACAGCTGGGGGAGT
  TCCAGTGCTGGTCACTAGAGGAGGGCTTCCAGCAGCTGGTAGCCAGCTACTGCCCAGAGGTGGTGGAGGA
  CGGGGTGGCAGACCAAACAGATGAGGGTGGCAGTGTACCCGTCATTATCAGCACATCGCGTGTGAGTGCA
  CCAGCTGGTGGCAAGGCCAGCTGGGGTGCAGACAGGTCCTACTGGAAGGAGTTCCTGGTGATGTGCACGC
  TCTTTGTGCTGGCCGTGCTGCTCCCAGTTTTATTCTTGCTCTACCGGCACCGGAACAGCATGAAAGTCTT
  CCTGAAGCAGGGGGAATGTGCCAGCGTGCACCCCAAGACCTGCCCTGTGGTGCTGCCCCCTGAGACCCGC
  CCACTCAACGGCCTAGGGCCCCCTAGCACCCCGCTCGATCACCGAGGGTACCAGTCCCTGTCAGACAGCC
  CCCCGGGGTCCCGAGTCTTCACTGAGTCAGAGAAGAGGCCACTCAGCATCCAAGACAGCTTCGTGGAGGT
  ATCCCCAGTGTGCCCCCGGCCCCGGGTCCGCCTTGGCTCGGAGATCCGTGACTCTGTGGTGTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_020210 unedited
  TGGCAAGGGGGACGAGTCAGTGGACACTCCAGGAAGAGCGGCCCCGCGGGGGGCGATGAC
  CGTGCGCTGACCCTGACTCACTCCAGGTCCGGAGGCGGGGGCCCCCGGGGCGACTCGGGG
  GCGGACCGCGGGGCGGAGCTGCCGCCCGTGAGTCCGGCCGAGCCACCTGAGCCCGAGCCG
  CGGGACACCGTCGCTCCTGCTCTCCGAATGCTGCGCACCGCGATGGGCCTGAGGAGCTGG
  CTCGCCGCCCCATGGGGCGCGCTGCCGCCTCGGCCACCGCTGCTGCTGCTCCTGCTGCTG
  CTGCTCCTGCTGCAGCCGCCGCCTCCGACCTGGGCGCTCAGCCCCCGGATCAGCCTGCCT
  CTGGGCTCTGAAGAGCGGCCATTCCTCAGATTCGAAGCTGAACACATCTCCAACTACACA
  GCCCTTCTGCTGAGCAGNGGATGGCAGGACCCTGGTACGTGGGTGGCTCGAGAGGCCCTC
  TTTGCACTCAGTAGCAACCTCAGCTTCCTGCCAGGCGGGGGAGTACCAGGAGAACATGGA
  GAACTTCACCCTGGCAGGGACGAGAGGGGGGAATGTCCTCCTGGAGATGGGAAGGNNNCC
  GTTGTCCCTTCGACCCGAATTTCAGTCCCTGCCCTGGNGGGNTGATGGCGAGCTCTACAC
  TGGNACAGTCAGCAGCTTCCAAGGAATGACCCCGGCTNNTGCGGAGCCAAAGCTTCGCCC
  CACAAGACCAGAGCTCCTTAACGGGCTGAGACCCACTTTTTGTGGCCCAACCTACATTCT
  GAAGACTGGGCACTTGCAGGCCATGATGACAAATT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_020210 unedited
  NAAATCTNTGNNACCGCGGCCGCAATCTAGNATCGCGTTTTTTTTTTTTTTTTTTAAACA
  GTAATTCTTCATACTTTATTAAAAAATGACATAAAGTGCATCTTATTAAAAAATATATAA
  AAACCACATAAATTCAGGGCCCCTGTGCTGGGCAGTGTTGATATCCCTTAGAGTGGAGGA
  AGGTGAGGGATGGAGGGTGAACTGGGGACTGGGGAGAGGACCAGGGTGCAGTTAGTTCCT
  CGTGTTTGAGTTCAAAGATGGAGCGAGGGTGGATATGGTGGGAAGGGGCACACGGGTTCT
  CACGCAACAACGGAGGAAGGCAGGCGACAGTCTCTTCCCTGAATTCTGAGGGAAACGCGT
  ACATTGTCCCGAAATCTCTCCTGAGCTCGCGCTGTCCTCTCGTGTGGCCACAGCCTGATA
  CAGGCTGGAAAGGTCCAGGAGTTGGGTCCGAGCCCAGGACCTGGTGAGGGCCGCAGCTCC
  AACCTAGCCCTCTGGTCCCTGAGGTGGTGGGACGACGGCAGCAACAACTACATCCTCGGC
  TGACTGGCAAGGCAGAACGCACGCAGCCCAAGCTGGTCCTGAATCTGCAGTGAGACAGGG
  CACCCGGTGGCAGCGGGATAATGTGGAAGGTGAAGAGGCCAGTTGGTGAGGGTTTCCAAC
  TCTGTCTCAGACACCCCTTTGGATGCATCCCCAGCACCCTGGGAAGCCTGAACTGGAGCA
  CCCGGGGAGCTGATGCCATGCTATCCCTTCCTTTAATCCAATTTCCATCTGAAATGCTTC
  CTCACCACGGCCTCCCTTACATACGAATATTGTTCTACAACCTCGCTGCCCCCCCTACTT
  CTTTCACCTTCTGGCCCATTTTCTCTGATTCCCCGTCCCCCCGCCTTNCTCCCCTTCTTC
  CCCTTTT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_020210
• Insert Size: 4000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_020210.2, NP_064595.2
• RefSeq Size: 3843 bp
• RefSeq ORF: 2514 bp
• Locus ID: 10509
• UniProt ID: Q9NPR2
• Cytogenetics: 15q26.1
• Protein Families: Transmembrane
• Protein Pathways: Axon guidance
• Gene Summary: Inhibits axonal extension by providing local signals to specify territories inaccessible for growing axons.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longest transcript. Variants 1 and 2 are predicted to encode the same protein which is annotated as the largest open reading frame. Translation may intitiate at either of the first two AUG sites. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.