CUTA (NM_015921) Human Untagged Clone

CAT#: SC127197

CUTA (untagged)-Human cutA divalent cation tolerance homolog (E. coli) (CUTA), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CUTA
• Synonyms: ACHAP; C6orf82
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC127197 sequence for NM_015921 edited (data generated by NextGen Sequencing)
  ATGCCGGCGCTGCTGCCTGTGGCCTCCCGCCTTTTGTTGCTACCCCGAGTCTTGCTGACC
  ATGGCCTCTGGAAGCCCTCCGACCCAGCCCTCGCCGGCCTCGGATTCCGGCTCTGGCTAC
  GTTCCGGGCTCGGTCTCTGCAGCCTTTGTTACTTGCCCCAACGAGAAGGTCGCCAAGGAG
  ATCGCCAGGGCCGTGGTGGAGAAGCGCCTAGCAGCCTGCGTCAACCTCATCCCTCAGATT
  ACATCCATCTATGAGTGGAAAGGGAAGATCGAGGAAGACAGTGAGGTGCTGATGATGATT
  AAAACCCAAAGTTCCTTGGTCCCAGCTTTGACAGATTTTGTTCGTTCTGTGCACCCTTAC
  GAAGTGGCCGAGGTAATTGCATTGCCTGTGGAACAGGGGAACTTTCCGTACCTGCAGTGG
  GTGCACCAGGTCACAGAGTCAGTTTCTGACTCTATCACAGTCCTGCCATGA
  
  Clone variation with respect to NM_015921.2
  425 g=>a
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_015921 unedited
  GCACGAGGGGCCGCATGAGTGGGGGGCGGGCTCCCGCGGTCCTGCTCGGCGGAGTGGCCT
  CTCTGCTCCTGTCTTTTGTTTGGATGCCGGCGCTGCTGCCTGTGGCCTCCCGCCTTTTGT
  TGCTACCCCGAGTCTTGCTGACCATGGCCTCTGGAAGCCCTCCGACCCAGCCCTCGCCGG
  CCTCGGATTCCGGCTCTGGCTACGTTCCGGGCTCGGTCTCTGCAGCCTTTGTTACTTGCC
  CCAACGAGAAGGTCGCCAAGGAGATCGCCAGGGCCGTGGTGGAGAAGCGCCTAGCAGCCT
  GCGTCAACCTCATCCCTCAGATTACATCCATCTATGAGTGGAAAGGGAAGATCGAGGAAG
  ACAGTGAGGTGCTGATGATGATTAAAACCCAAAGTTCCTTGGTCCCAGCTTTGACAGATT
  TTGTTCGTTCTGTGCACCCTTACGAAGTGGCCGAGGTAATTGCATTGCCTGTGGAACAGG
  GGAACTTTCCGTACCTGCAGTGGGTGCACCAGGTCACAGAGTCAGTTTCTGACTCTATCA
  CAGTCCTGCCATGATGAGCCCTGTTCCTGCTCATCATGAAGATCCCCGCGATACTTCAAC
  GCCTTCTGACTTCCAGGTGATGACTGGGCCCCCAATAAATCCCGTCTTTGGG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_015921
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_015921.2, NP_057005.1
• RefSeq Size: 1214 bp
• RefSeq ORF: 471 bp
• Locus ID: 51596
• UniProt ID: O60888
• Cytogenetics: 6p21.32
• Domains: CutA1
• Protein Families: Transmembrane
• Gene Summary: May form part of a complex of membrane proteins attached to acetylcholinesterase (AChE).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) uses an alternate splice site in the 5' coding region, compared to variant 1, resulting in a shorter protein (isoform 2). Isoform 2 has also been called isoform c. Variants 2, 3 and 4 encode the same protein.