DYNLRB1 (NM_177953) Human Untagged Clone

CAT#: SC126983

DNCL2A (untagged)-Human dynein, cytoplasmic, light polypeptide 2A (DNCL2A), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: DYNLRB1
• Synonyms: BITH; BLP; DNCL2A; DNLC2A; ROBLD1
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_177953, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGACAACCCCACCACCACCCAGTATGCCAGCCTCATGCACAGCTTCATCCTGAAGGCACGGAGCACCG
  TGCGTGACATCGACCCCCAGAACGATCTCACCTTCCTTCGAATTCGCTCCAAGAAAAATGAAATTATGGT
  TGCACCAGATAAAGACTATTTCCTGATTGTGATTCAGAATCCAACCGAATAA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_177953 unedited
  TGTTATACGACTCATATAGGCGGCCGCGNAATTCGCACGAGGCGCGGGGCTACCGGATCG
  GTCGGAATGGCAGAGGTGGAGGAGACACTGAAGCGACTGCAGAGCCAGAAGGGAGTGCAG
  GGAATCATCGTCGTGAACACAGAAGGCATTCCCATCAAGAGCACCATGGACAACCCCACC
  ACCACCCAGTATGCCAGCCTCATGCACAGCTTCATCCTGAAGGCACGGAGCACCGTGCGT
  GACATCGACCCCCAGAACGATCTCACCTTCCTTCGAATTCGCTCCAAGAAAAATGAAATT
  ATGGTTGCACCAGATAAAGACTATTTCCTGATTGTGATTCAGAATCCAACCGAATAAGCC
  ACTCTCTTGGCTCCCTGTGTCATTCCTTAATTTAATGCCCCCCAAGAATGTTAATGTCAA
  TCATGTCAGTGGACTAGCACATGGCAGTCGCTTGGAACCCACTCACACCAATCCAGTGAC
  CGTGTGTGGGCTGGCGGCTCTTCTCCCCCACCAACGGAACCCCTGTGTGCACCAACCTTC
  CCCAGAGCTCCGGAGCGCCCTCTCCTCACTTCCAGGTTTTGGAGCAAGAGCTTGCAGGAA
  GCCCGCACCCAGCTTCCTTCTGACCTTCAGTTCACTTTGTCGCCCTTGGAGAAAGCTGTT
  TTTCTTTAACTAAAAATAACCAAAATGCTAAAAAAAAAAAAAAAAAACTCCAGACTAATT
  CTCCCACAATGGTAGAAAATGCAGATTTGAATAGATTAAGCATTTAAGGGTGAAGAAACA
  AANAACATCTTTTAACTTGACACTTCAAGGTTTAAAATTCTTCAGTGGCTTCCCACTGTT
  TTTGGNACAGTCTATACTCTTGCATGGNCACGTGAGATCTTTCACAATNTGG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_177953
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_177953.1, NP_808852.1
• RefSeq Size: 796 bp
• RefSeq ORF: 192 bp
• Locus ID: 83658
• UniProt ID: Q9NP97
• Cytogenetics: 20q11.22
• Gene Summary: This gene is a member of the roadblock dynein light chain family. The encoded cytoplasmic protein is capable of binding intermediate chain proteins, interacts with transforming growth factor-beta, and has been implicated in the regulation of actin modulating proteins. Upregulation of this gene has been associated with hepatocellular carcinomas, suggesting that this gene may be involved in tumor progression. Alternative splicing results in multiple transcript variants. Pseudogenes of this gene have been defined on chromosomes 12 and 18. [provided by RefSeq, Aug 2013]
  Transcript Variant: This variant (2) differs in its 5' UTR and initiates translation at a downstream start codon, compared to variant 1. The encoded isoform (b) has a shorter N-terminus, compared to isoform a. Variants 2, 3, and 4 encode the same isoform.