CD147 (BSG) (NM_198589) Human Untagged Clone

CAT#: SC126562

BSG (untagged)-Human basigin (Ok blood group) (BSG), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CD147
• Synonyms: 5F7; CD147; EMMPRIN; EMPRIN; HAb18G; OK; SLC7A11; TCSF
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC126562 sequence for NM_198589 edited (data generated by NextGen Sequencing)
  ATGGCGGCTGCGCTGTTCGTGCTGCTGGGATTCGCGCTGCTGGGCACCCACGGAGCCTCC
  GGGGCTGCCGGCACAGTCTTCACTACCGTAGAAGACCTTGGCTCCAAGATACTCCTCACC
  TGCTCCTTGAATGACAGCGCCACAGAGGTCACAGGGCACCGCTGGCTGAAGGGGGGCGTG
  GTGCTGAAGGAGGACGCGCTGCCCGGCCAGAAAACGGAGTTCAAGGTGGACTCCGACGAC
  CAGTGGGGAGAGTACTCCTGCGTCTTCCTCCCCGAGCCCATGGGCACGGCCAACATCCAG
  CTCCACGGGCCTCCCAGAGTGAAGGCTGTGAAGTCGTCAGAACACATCAACGAGGGGGAG
  ACGGCCATGCTGGTCTGCAAGTCAGAGTCCGTGCCACCTGTCACTGACTGGGCCTGGTAC
  AAGATCACTGACTCTGAGGACAAGGCCCTCATGAACGGCTCCGAGAGCAGGTTCTTCGTG
  AGTTCCTCGCAGGGCCGGTCAGAGCTACACATTGAGAACCTGAACATGGAGGCCGACCCC
  GGCCAGTACCGGTGCAACGGCACCAGCTCCAAGGGCTCCGACCAGGCCATCATCACGCTC
  CGCGTGCGCAGCCACCTGGCCGCCCTCTGGCCCTTCCTGGGCATCGTGGCTGAGGTGCTG
  GTGCTGGTCACCATCATCTTCATCTACGAGAAGCGCCGGAAGCCCGAGGACGTCCTGGAT
  GATGACGACGCCGGCTCTGCACCCCTGAAGAGCAGCGGGCAGCACCAGAATGACAAAGGC
  AAGAACGTCCGCCAGAGGAACTCTTCCTGA
  
  Clone variation with respect to NM_198589.1
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_198589 unedited
  GTATACGACTCACTAAGGGCGGCCGCGACTTCGGCACGAGGGGTTGTAGGACCGGCGAGT
  GATAGGAATCATGGCGGCTGCGCTGTTCGTGCTGCTGGGATTCGCGCTGCTGGGCACCCA
  CGGAGCCTCCGGGGCTGCCGGCACAGTCTTCACTACCGTAGAAAGACCTTGGCTCCAAGA
  TACTCCTCACCTGCTCCTTGAATGACAGCGCCACAGAGGTCACAGGGCACCGCTGGCTGA
  AGGGGGGCGTGGTGCTGAAGGAGGACGCGCTGCCCGGCCAGAAAACGGAGTTCAAGGTGG
  ACTCCGACGACCAGTGGGGAGAGTACTCCTGCGTCTTCCTCCCCGAGCCCATGGGCACGG
  CCAACATCCAGCTCCACGGGCCTCCCAGAGTGAAGGCTGTGAAGTCGTCAGAACACATCA
  ACGAGGGGGAGACGGCCATGCTGGTCTGCAAGTCAGAGTCCGTGCCACCTGTCACTGACT
  GGGCCTGGTACAAGATCACTGACTCTGAGGACAAGGCCCTCATGAACGGCTCCGAGAGCA
  GGTTCTTCGTGAGTTCCTCGCAGGGCCGGTCAGAGCTACACATTGAGAACCTGAACATGG
  AGGCCGACCCCGGCCAGTACCGGTGCAACGGCACCAGCTNCAGGGCTCCGACCAGGCCAT
  CATCACGCTCCGCGTGCGCAGCCACCTGGCCGGCCTTCTGCCCTTTCTGGGCATCGTGGC
  TGAAGTGCTGGTGCTGGNCACCATCATCTTCATCTACAAAAAGCGCGGGAGCCCGAGGAC
  GTCCTGGAGATGACCAAGCCCGCTTTGGACCCTGAAAAGAACGGGCAGACCAGATGACAA
  GGCANAACTCCGCAGAGAACTTTCTGGAGGGGCCGAGAACTTCCTGGTCCATTTGGCCCC
  GCCGAAACCATCCAAGCTTC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_198589
• Insert Size: 1670 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_198589.1, NP_940991.1
• RefSeq Size: 1676 bp
• RefSeq ORF: 810 bp
• Locus ID: 682
• UniProt ID: P35613
• Cytogenetics: 19p13.3
• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Transmembrane
• Gene Summary: The protein encoded by this gene, basigin, is a plasma membrane protein that is important in spermatogenesis, embryo implantation, neural network formation, and tumor progression. Basigin is also a member of the immunoglobulin superfamily, ubiquitously expressed in various tissues. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2020]
  Transcript Variant: This variant (2) lacks an alternate in-frame exon compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is shorter compared to isoform 1. Variants 2 and 5 both encode the same isoform (2).