ETFBKMT (NM_173802) Human Untagged Clone

CAT#: SC126245

METTL20 (untagged)-Human methyltransferase like 20 (METTL20), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ETFBKMT
• Synonyms: C12orf72; ETFB-KMT; METTL20
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_173802 edited
  GCCGCGAGGGGCCGCCGTCCGTCCCGCCCCCTGCCGGGCCGCGGCGCTCTGCTAGAGCGT
  GGCTCAGTTTTGGTAGCGGGACCCCGGAAATGTAAAGCATGAGCGGGAACAGTCGCCAGG
  GCGAGTGACAGGCGAGCCCTATGCTTTACTTCCGAGGGCGGTGTCAGCAAAGCTAGAGTC
  AGAGGTTCCGGTTGAGATCAAGTTGGGAGACACACCCTTGTTCATTCTCCTTGAGAAGCA
  GCTATTATCAACAGAACATTGACAGAACCTGTGTTTGGGGAAAGGACTGATGGCTTTGAG
  TCTAGGTTGGAAAGCACACAGGAACCACTGTGGTCTCCTCTTGCAGGCTCTGCGAAGCAG
  TGGTCTTCTCTTGTTTCCCTGTGGCCAGTGTCCCTGGAGAGGAGCTGGAAGCTTTTTGGA
  CCCTGAGATAAAGGCTTTCCTGGAGGAGAACACTGAAGTCACCAGCAGTGGTAGCCTCAC
  CCCTGAAATCCAGTTGCGGCTTTTGACCCCCAGATGCAAATTCTGGTGGGAGAGAGCTGA
  CCTGTGGCCCCACAGTGATCCTTACTGGGCAATCTACTGGCCAGGAGGCCAAGCCCTGTC
  TAGGTATCTTTTGGATAATCCTGATGTTGTCAGAGGAAAATCTGTATTAGATCTTGGGAG
  TGGATGTGGAGCTACAGCTATTGCTGCTAAGATGAGTGGGGCATCAAGGATCTTGGCCAA
  TGACATAGACCCTATTGCAGGAATGGCTATTACACTAAATTGTGAATTGAACAGACTGAA
  TCCTTTTCCTATTTTAATCCAAAACATTTTGAATTTGGAACAAGATAAGTGGGACCTTGT
  TGTTCTTGGCGATATGTTTTATGATGAAGACCTTGCAGATAGTCTTCATCAGTGGCTGAA
  GAAGTGCTTCTGGACCTATAGAACTCGAGTACTGATTGGTGACCCTGGGCGGCCCCAGTT
  CAGTGGACACAGCATTCAGCATCACCTGCACAAAGTGGTAGAATATTCACTTTTGGAGTC
  TACTAGGCAGGAAAACAGTGGACTGACAACAAGCACAGTGTGGGGTTTTCAGCCTTGAGT
  TGTCAAAGTGCTTCCAAGTATGAATATAGTAATGTTTGGATCTGACTCTAAAAGTTTTCT
  CTATAGGAGATACTCTCCAGTTTAATGAATGTAATTCTTGTTAAATGGAAAATCTTGTTT
  TTAAAATATGAAGGTTTAGAGTTTTGTTTACTTTTGTCATGTAACTGGTTCTGTATTTCT
  ATGCATGCCAATTATACACATCTCTATCTGCATTTTTTTTTCTATTTTAACCGCTGAAGG
  AATATGATTATACAATGCCATACTCAATGGTGGCAGCATTTAATTTAAGTAATGGAGAAG
  AATTAAACATTGTAGCTGGATCTGTTTCTGTAGTTGAGGACAACTGTGATGTAACTTTGA
  ATTAAACAAACAAAACAAAACAGAAGAAGACTATATATATATTTTTTCTTTTTTTAGAGA
  CAGTCTCACTTTGCTCCCAGGCTGGAATGCAGTGGCTATTCAGAGGCTTAATCATTGGGC
  ACTGCAGCCTTGAACTCTGGGCTTTTTTTTTTTTTGAGATGGAGTCTCAGCTCTGTCGTC
  CAGGCTGGAGTGCAGTGACTCATGTCTCGGCTCACTGTAGCCTCCACCTCCCCCAATCAA
  GCGATTGTCCTGTCTCAGCCTCCAGAGTAGCTGGGATTTTAGGTGTGCACCACCATGCCT
  GGCTAATTTTTGTATTTTTAGTAGAGACGGGGTTTTGCCATGTTGGCCAGGCCGGTCTCA
  AACTCCTGACCTCAGGTGATCCACCCGCCTTGGCCTCCCAAAGTGCTAGGATTACAGGTG
  TGAGCCACCACGCCAGCCTGACGATGTTTTTTACTTCTATCACTTCCTTTTGATTTTTTC
  TTATAGCTTCTATCTCTGCTTACGTCACCCATCATTCTTGCGTGTTGTATGCTTTTCGCA
  TTAGAGCCCTTTGCATGTTAATCAGTTATTTTAAATTTATGATCTGGTAATTCTAAAATG
  TCTGCCATAACTGAAAAAAAAAAAAAAAAAAAAAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_173802 unedited
  NNNGGTAGTTCTTGATTTGTNATACGACTTACTATAGGCGGCCGCGGATTCANATCTGGT
  ACCGGTCCGGAATTCCCGGGATGCCGCGAGGGGCCGCCGTCCGTCCCGCCCCCTGCCGGG
  CCGCGGCGCTCTGCTAGAGCGTGGCTCAGTTTTGGTAGCGGGACCCCGGAAATGTAAAGC
  ATGAGCGGGAACAGTCGCCAGGGCGAGTGACAGGCGAGCCCTATGCTTTACTTCCGAGGG
  CGGTGTCAGCGAAGCTAGAGTCAGAGGTTCCGGTTGAGATCAAGTTGGGAGACACACCCT
  TGTTCATTCTCCTTGAGAAGCAGCTATTATCAACAGAACATTGACAGAACCTGTGTTTGG
  GGAAAGGACTGATGGCTTTGAGTCTAGGTTGGAAAGCACACAGGAACCACTGTGGTCTCC
  TCTTGCAGGCTCTGCGAAGCAGTGGTCTTCTCTTGTTTCCCTGTGGCCAGTGTCCCTGGA
  GAGGAGCTGGAAGCTTTTTGGACCCTGAGATAAAGGCTTTCCTGGAGGAGAACACTGAAG
  TCACCAGCAGTGGTAGCCTCACCCCTGAAATCCAGTTGCGGCTTTTGACCCCCAGATGCA
  AATTCTGGTGGGAGAGAGCTGACCTGTGGCCCCACAGTGATCCTTACTGGGCAATCTACT
  GGCCAGGAGGCCAAGCCCTGTCTAGGTATCTTTTGGATAATCCTGATGTTGTCAGAGGAA
  AATCTGTATTAGATCTTGGGAGTGGATGTGGAGCTACAGCTATTGCTGCTAAGATGAGTG
  GGGCATCAAGGATCTTGGCCAATGACATAGACCCTATTGCAGGAATGGCTATTACACTAA
  ATTGTGAATTGAACAGACTGAATCCTTTTTCCTAT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_173802
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_173802.2, NP_776163.1
• RefSeq Size: 2078 bp
• RefSeq ORF: 789 bp
• Locus ID: 254013
• UniProt ID: Q8IXQ9
• Cytogenetics: 12p11.21
• Protein Families: Druggable Genome
• Gene Summary: Protein-lysine methyltransferase that selectively trimethylates the flavoprotein ETFB in mitochondria (PubMed:25023281, PubMed:25416781). Thereby, may negatively regulate the function of ETFB in electron transfer from Acyl-CoA dehydrogenases to the main respiratory chain (PubMed:25416781).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longest transcript. Variants 1, 2, and 3 encode the same protein. Sequence Note: The RefSeq transcript and protein were derived from transcript and genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.