PRAS40 (AKT1S1) (BC016043) Human Untagged Clone

CAT#: SC126155

AKT1S1 (untagged)-Human AKT1 substrate 1 (proline-rich) (cDNA clone MGC:20246 IMAGE:4635389), complete cds

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PRAS40
• Synonyms: AKT1 substrate 1 (proline-rich); Lobe; MGC2865; OTTHUMP00000196757; OTTHUMP00000196758; OTTHUMP00000196759; OTTHUMP00000196760; PRAS40; proline-rich Akt substrate, 40 kDa
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_001098632 edited
  GGATAACGGGATGAAGCCGTCAGTGCCCCCAGAAACGAAGCGGCCCCGGACGAATTTCTG
  AGTCACCGTCGCGAGAAAGCGGGCTGAGCCGCCATTTTGAAGCCTGGCAAACCGAAGCAA
  GAAATGCTGCCGTGTTGGATCTTTGCCAGCCTTCGTGCCGAATGGGAGCAGGGCGCGGAT
  GGCGTCGGGGCGCCCCGAGGAGCTGTGGGAGGCCGTGGTGGGGGCCGCTGAGCGCTTCCG
  GGCCCGGACTGGCACGGAGCTGGTGCTGCTGACCGCGGCCCCGCCGCCACCACCCCGCCC
  GGGCCCCTGTGCCTATGCTGCCCATGGTCGAGGAGCCCTGGCGGAGGCAGCGCGCCGTTG
  CCTCCACGACATCGCACTGGCCCACAGGGCTGCCACTGCTGCTCGGCCTCCTGCGCCCCC
  ACCAGCACCACAGCCACCCAGTCCCACACCCAGCCCACCCCGGCCTACCCTGGCCAGAGA
  GGACAACGAGGAGGACGAGGATGAGCCCACAGAGACAGAGACCTCCGGGGAGCAGCTGGG
  CATTAGTGATAATGGAGGGCTCTTTGTGATGGATGAGGACGCCACCCTCCAGGACCTTCC
  CCCCTTCTGTGAGTCAGACCCCGAGAGTACAGATGATGGCAGCCTGAGCGAGGAGACCCC
  CGCCGGCCCCCCCACCTGCTCAGTGCCCCCAGCCTCAGCCCTACCCACACAGCAGTACGC
  CAAGTCCCTGCCTGTGTCTGTGCCCGTCTGGGGCTTCAAGGAGAAGAGGACAGAGGCGCG
  GTCATCAGATGAGGAGAATGGGCCGCCCTCTTCGCCCGACCTGGACCGCATCGCGGCGAG
  CATGCGCGCGCTGGTGCTGCGAGAGGCCGAGGACACCCAGGTCTTCGGGGACCTGCCACG
  GCCGCGGCTTAACACCAGCGACTTCCAGAAGCTGAAGCGGAAATATTGAAGTCCAGGGAG
  GGAGCGCCCCGGGCCGCGTCCGCCCCGTCCCACACTACGCCCCCGCCCCACTCCCGGGGC
  CTGCTAATCTGAGGCCGATCCGGGACCGGCCTCCTTGCGTCTCCCATTCCCAATTGGCTC
  CAGCTTCCTGGGGGCGTGGCCAAGCCCTCCTCTTCCCAGAATTGGCCCGGGGCCTTCAAT
  TTACGTTCTTTACACTACGGGGACTGGGGTCGTCTTTGCCCACGTCCCGACAACTTGTTC
  CCTGACCCCCTCAGGGATGGCCCCAAACTGTCCCTGCCTCTGGCACCCCCTTTCATTGGT
  TCCATCCATCCCCACAACAGCCTGCCAATCGAAGCCCGTCCCTGCATCCAGGATGGTACC
  AGCTCCCGCCCCTCGCCCCCCACCTCCACAGGTGCCTTAAAGGGCCCTCGTCCACCCAAG
  GTGGGGGGCAGGGGCCCTCACTCTCCGGCCCTGGTGTGGGGGAGAGAGTGAGGGGTTGGG
  GGATCGGCAGTTGGGAGGGGCGCTCTGAGATTAAAGAGTTTTACCTCTGAGATAAAAAAA
  AAAAAAAAAAA
  
• Restriction Sites: Please inquire
• ACCN: BC016043
• Insert Size: 1500 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC016043.1, AAH16043.1
• RefSeq Size: 1511 bp
• Locus ID: 84335
• Cytogenetics: 19q13.33
• Gene Summary: AKT1S1 is a proline-rich substrate of AKT (MIM 164730) that binds 14-3-3 protein (see YWHAH, MIM 113508) when phosphorylated (Kovacina et al., 2003 [PubMed 12524439]).[supplied by OMIM, Mar 2008]