SPO11 (NM_198265) Human Untagged Clone

CAT#: SC125697

SPO11 (untagged)-Human SPO11 meiotic protein covalently bound to DSB homolog (S. cerevisiae) (SPO11), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SPO11
• Synonyms: CT35; SPATA43; TOPOVIA; TOPVIA
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_198265 edited
  CAAGGGCGCAGCCTAGGACAGGGGCTTCTGGAGCTTCTGGCAGCCGTCTGCCCTCATGGC
  CTTTGCACCTATGGGGCCCGAGGCCTCGTTCTTCGACGTTTTGGACCGACACAGGGAGTC
  CCTGCTGGCTGCCCTGAGGAGAGGTGGCAGGGAGCCCCCAACTGGGGGAAGCCGCCTGGC
  CTCCAGGTTTGAAGATTCTGTGGGTCTTCAGATGGTATCCCATTGCACCACCAGAAAGAT
  CAAAAGTGATTCACCAAAATCAGCTCAAAAATTTTCTCTAATCCTTAAAATATTGTCCAT
  GATTTATAAATTAGTACAGAGCAACACTTATGCAACCAAAAGGGACATATATTACACTGA
  CAGTCAACTCTTTGGTAACCAGACTGTCGTCGACAATATTATCAATGACATTTCTTGCAT
  GTTAAAAGTGTCAAGGAGGAGTCTACATATATTATCTACATCAAAAGGTTTAATTGCTGG
  CAACTTAAGATACATCGAGGAAGATGGCACCAAAGTGAATTGTACCTGTGGTGCAACGGC
  TGTTGCTGTGCCATCGAATATTCAAGGAATTCGGAATTTAGTTACAGATGCAAAGTTTGT
  ATTAATTGTAGAAAAAGATGCAACATTTCAGCGGCTCCTAGATGACAACTTTTGCAACAA
  ATTGTCTCCTTGCATCATGATTACGGGAAAGGGAGTTCCTGATCTAAACACAAGACTTTT
  AGTCAAGAAACTGTGGGATACATTTCATGTTCCTGTTTTCACTCTTGTAGATGCTGATCC
  ACATGGCATAGAAATAATGTGCATCTATAAGTATGGATCTATGTCTATGTCTTTTGAAGC
  TCATCATCTCACAGTTCCAGCTATTAGATGGCTTGGTCTTCTCCCTTCTGATCTTAAAAG
  ATTAAATGTACCTAAAGATAGTTTGATTCCACTGACAAAAAGGGACCAAATGAAACTTGA
  CAGTATCCTGAGGAGACCTTATGTTACCTGCCAACCATTTTGGAGAAAAGAAATGGAAAT
  AATGGCAGACTCTAAAATGAAGGCAGAAATTCAAGCTTTGACTTTCCTATCATCAGATTA
  TCTTTCCAGAGTGTACTTACCTAACAAATTAAAATTTGGAGGATGGATATAAAAATAAAT
  CAGAAGAACTTCTGATTGCCAGAGGCTTTTCATTAGTTTTGTTTTGATTGGCAAATACTA
  TTGTGGAAAGAACATATATTATATTCTTAATTCTGTAAAAGTGAAATAAAATAACTTTCC
  GTTAATTATATATTTTTGTCAAAACAAATGCTGTACTCCAATTTTCTTTGCAAGGCCTTA
  TTCTTGCCTCTATAGAGACAGATTTCTGTCCTATCTTCTAAAGCAAATTATAAAAGAATA
  TGTTATTTTGACCTTTAAATTATTTTTGAAAAAATAATATTTTATACATGTCATCAAAGT
  CTACAAAATATTTACCTTCTACGATACAACTAATGTTAACGCATAAAGTATCTTACTGGT
  AACAAAAATCATAATGATCTGAATTTGAGATGTTGCAAATGAATTGTGGTGTCCGGTAGT
  TTCTTCTTACATTTTCCTTTGCCTTTATACTTTAGGGGTCTTACTCCATTAATTCATTTG
  TTACATTAGTAAAATTCAGTATGAATAAATATTTGGATTGATGTAAAAAAAAAAAAAAAA
  AAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_198265 unedited
  GTTTTATTTGTATACCATCATATAGGCGGACCGCGAATTCGATCTGGTACCGGTCCGGAT
  TCCCGGGATCAAGGCGCAGCCTAGGACAGGGGCTTCTGGAGCTTCTGGCAGCCGTCTGCC
  CTCATGGCCTTTGCACCTATGGGGCCCGAGGCCTCGTTCTTCGACGTTTTGGACCGACAC
  AGGGAGTCCCTGCTGGCTGCCCTGAGGAGAGGTGGCAGGGAGCCCCCAACTGGGGGAAGC
  CGCCTGGCCTCCAGGTTTGAAGATTCTGTGGGTCTTCAGATGGTATCCCATTGCACCACC
  AGAAAGATCAAAAGTGATTCACCAAAATCAGCTCAAAAATTTTCTCTAATCCTTAAAATA
  TTGTCCATGATTTATAAATTAGTACAGAGCAACACTTATGCAACCAAAAGGGACATATAT
  TACACTGACAGTCAACTCTTTGGTAACCAGACTGTCGTCGACAATATTATCAATGACATT
  TCTTGCATGTTAAAAGTGTCAAGGAGGAGTCTACATATATTATCTACATCAAAAGGTTTA
  ATTGCTGGCAACTTAAGATACATCGAGGAAGATGGCACCAAAGTGAATTGTACCTGTGGT
  GCAACGGCTGTTGCTGTGCCATCGAATATTCAAGGAATTCGGAATTTAGTTACAGATGCA
  AAGTTTGTATTAATTGTAGAAAAAGATGCCACATTTCAGCGGCTCCTAGATGACAACTTT
  TGCAACANATTGTCTCCTTGCATCATGATTACCGGAAAGGGAGTTCCTGATCTANACACA
  AGACTTTTAGTCAAGAAACTGTGGGATACATTTCATGTTCCTGTTTTCACTCTTGTAGAT
  GCTGATCCACATGGCATAGAAAATATGTGCATCTATAAGTATGGATCTATGTCTATGTCT
  TTTGAA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_198265
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_198265.1, NP_937998.1
• RefSeq Size: 1712 bp
• RefSeq ORF: 1077 bp
• Locus ID: 23626
• UniProt ID: Q9Y5K1
• Cytogenetics: 20q13.31
• Protein Families: Druggable Genome, Transcription Factors
• Gene Summary: Meiotic recombination and chromosome segregation require the formation of double-strand breaks (DSBs) in paired chromosome homologs. During meiosis in yeast, a meiotic recombination protein is covalently-linked to the 5' end of DSBs and is essential for the formation of DSBs. The protein encoded by this gene is similar in sequence and conserved features to the yeast meiotic recombination protein. The encoded protein belongs to the TOP6A protein family. Several transcript variants encoding different isoforms have been found for this gene, but the full-length nature of only two of them have been described. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (2) lacks an alternate in-frame exon compared to variant 1. The resulting isoform (b) lacks an internal segment compared to isoform a.