Carboxypeptidase Z (CPZ) (NM_003652) Human Untagged Clone

CAT#: SC125589

CPZ (untagged)-Human carboxypeptidase Z (CPZ), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Carboxypeptidase Z
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC125589 sequence for NM_003652 edited (data generated by NextGen Sequencing)
  ATGCCGCCCCCGCCGCCGCTGCTGCTCCTTACAGTCCTGGTCGTCGCCGCTGCCCGGCCG
  GGGTGCGAGTTTGAGCGGAACCCCGCCGCCACCTGCGTGGACCTGCAGCTCAGGACCTGC
  AGCGATGCCGCCTACAACCACACCACCTTCCCCAACCTGCTTCAGCACCGGTCGTGGGAG
  GTGGTGGAGGCCAGCTCCGAGTACATCCTGCTGAGCGTTCTACACCAGCTCCTGGAAGGC
  CAGTGCAACCCGGACCTGCGGCTGCTGGGCTGTGCTGTGCTGGCCCCCCGGTGTGAGGGC
  GGCTGGGTGCGCAGACCCTGCCGGCACATCTGCGAGGGCCTGCGGGAGGTCTGCCAGCCC
  GCCTTCGACGCCATTGACATGGCYTGGCCCTACTTCCTTGACTGCCACCGCTACTTCACG
  AGAGAGGACGAGGGCTGCTATGACCCGCTGGAGAAGCTTCGGGGAGGCCTGGAGGCTGAC
  GAGGCACTGCCCTCAGGGCTGCCGCCCACCTTCATCCGCTTCAGCCACCACTCCTACGCC
  CAGATGGTGCGTGTGCTGAGGCGGACGGCCTCCCGCTGTGCCCACGTGGCCAGGACCTAC
  AGCATCGGGCGCAGCTTCGACGGCAGGGAGCTGCTGGTCATCGAGTTCTCCAGCCGCCCC
  GGCCAGCACGAGCTGATGGAGCCCGAGGTGAAGCTCATCGGCAACATTCATGGCAACGAG
  GTGGCGGGCCGGGAGATGCTCATCTACCTAGCCCAGTACCTGTGCTCTGAGTACCTGCTT
  GGTAACCCCCGCATCCAGCGCCTGCTCAACACCACCCGCATCCACCTGCTGCCCTCCATG
  AACCCTGACGGCTATGAGGTGGCAGCTGCCGAGGGTGCCGGCTACAACGGGTGGACGAGC
  GGGAGGCAGAACGCGCAGAACCTGGATCTGAACCGAAATTTCCCGGACCTGACGTCCGAG
  TACTACCGGCTGGCGGAGACCCGCGGCGCACGCAGCGACCACATCCCCATCCCCCAGCAC
  TACTGGTGGGGTAAGGTGGCCCCGGAGACAAAGGCAATCATGAAGTGGATGCAGACCATA
  CCCTTTGTGCTCTCAGCCAGCCTTCATGGGGGCGACCTGGTGGTGTCCTACCCCTTCGAC
  TTCTCCAAGCACCCCCAGGAGGAGAAGATGTTTTCTCCCACGCCCGACGAGAAGATGTTC
  AAGCTGCTGTCCAGAGCCTACGCTGATGTCCACCCCATGATGATGGACAGGTCGGAGAAT
  AGGTGTGGAGGCAATTTCCTGAAGAGGGGGAGCATCATCAACGGGGCAGACTGGTACAGC
  TTCACGGGAGGCATGTCCGACTTCAACTACCTGCACACCAACTGCTTTGAGATCACGGTA
  GAGCTGGGCTGTGTGAAGTTCCCCCCCGAGGAGGCCCTGTACACACTCTGGCAGCACAAC
  AAGGAGTCACTCCTGAATTTCGTGGAGACGGTGCACCGGGGCATCAAAGGTGTGGTGACA
  GATAAATTCGGCAAGCCAGTCAAAAACGCCCGGATCTCAGTCAAAGGCATTCGCCACGAC
  ATCACCACAGCCCCAGATGGTGACTACTGGAGACTGCTGCCCCCAGGTATCCACATTGTC
  ATTGCCCAAGCCCCTGGCTACGCCAAAGTCATCAAGAAAGTCATCATCCCCGCCCGGATG
  AAGAGGGCTGGCCGTGTGGACTTCATTCTGCAACCTCTGGGGATGGGACCCAAGAACTTT
  ATTCATGGGCTGCGGAGGACTGGGCCCCACGACCCGCTGGGAGGTGCCAGCTCTTTGGGG
  GAGGCCACGGAGCCCGACCCGCTCCGGGCGCGCAGGCAGCCCTCGGCCGACGGGAGTAAG
  CCCTGGTGGTGGTCCTACTTCACATCGCTGAGCACCCACAGGCCACGCTGGCTGCTCAAG
  TACTAG
  
  Clone variation with respect to NM_003652.3
  384 c=>y;579 c=>t;1227 c=>t;1308 g=>a;1341 t=>c
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003652 unedited
  TCGGCATTTTGTAATACGACTTACTATAGGGCGGCACGCGATTCGGCACCAGGCCGCCCC
  ACCATGCCGCCCCCGCCGCCGCTGCTGCTCCTTACAGTCCTGGTCGTCGCCGCTGCCCGG
  CCGGGGTGCGAGTTTGAGCGGAACCCCGCCGCCACCTGCGTGGACCTGCAGCTCATGACC
  TGCAGCGATGCCGCCTACAACCACACCACCTTCCCCAACCTGCTTCATCACCGGTCGTGG
  GATGTGGTGGAGGCCATCTCCGAGTACATCCTGCTGAGCGTTCTACACCAGCTCCTGGAA
  GGCCAGTGCAACCCGGACCTGCGGCTGCTGGGCTGTGCTGTGCTGGCCCCCCGGTGTGAT
  GGCGGCTGGGTGCGCATACCCTGCCGGCACATCTGCGATGGCCTGCGGGATGTCTGCCAG
  CCCGCCTTCGACGCCATTGACATGGCCTGGCCCTACTTCCTTGACTGCCACCGCTACTTC
  ACGAGATATGACGATGGCTGCTATGACCCGCTGGAGAATCTTCGGAGATGCCTGNATGCT
  GACGATGCACTGCCCTCATGGCTGCCGCCCACCTTCATTCGCTTCATCCACCACTTCTAC
  GCCCATATGGTGCGTGTGCTGATGCAGACAGGCTNCCGCTGTGCCCACGTGGCCATGAAT
  CTACAGCATCGGGCGCATCTTCAACGGCAAGGAGCTGCTGGTCATCGAGTTCTTCAGCCC
  GCCCGGNCATCACGATCTGATGGAGCCCGATGTGAATNCTCATCNGCCATCATTCATGGC
  AATCGATGTGGGCGGGGCCGCGGAGATGCTCANTCTACCTATCCCCATAACCTGGTGCTT
  CTGAGGTACCTGGCTTGGGTATCCCCCGCATTCATANGGCTGGCTTAAAATCCACCCGAA
  TTCACCTGGCTGGCCCTCCATGAAACCCCTGACGGCTTTTAAGGTGGCANCTGCCCGAGG
  TGGCCGGCTACAACCGGTGGAACAAACCGGAGAGCCAACGCCCAATAACTGGGATTGGAC
  CCTATTTCTCCGGAACTGAC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_003652 unedited
  TCCGGGCCAGGAGAGGCACTGGGGAGGGGTCACAGGGATGCCACCCGGGATCTGTTCAGG
  AAACAGCTATGACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTCCAGTG
  GAAAGTGGATCAAAACACTTTAATAAAATGGCAGCGGCTTTGTGGGATGTCTGTGGCAGA
  CAAAATCAAGAGCCAGGAGCCCGGGATGCGGAGATGGGCCTCGGTCTCCACATCCTGGCG
  GGTGCTGGGGCCGGGGCTAGTACTTGAGCAGCCAGCGTGGCCTGTGGGTGCTCAGCGATG
  TGAAGTAGGACCACCACCAGGGCTTACTCCCGTCGGCCGAGGGCTGCCTGCGCGCCCGGA
  GCGGGTCGGGCTCCGTGGCCTCCCCCAAAGAGCTGGCACCTCCCAGCGGGTCGTGGGGCC
  CAGTCCTCCGCAGCCCATGAATAAAGTTCTTGGGTCCCATCCCCAGAGGTTGCAGAATGA
  AGTCCACACGGCCAGCCCTCTTCATCCGGGCGGGGATGATGACTTTCTTGATGACTTTGG
  CGTAGCCAGGGGCTTGGGCAATGACAATGTGGATACCTGGGGGCAGCAGTCTCCAGTAGT
  CACCATCTGGGGCTGTGGTGATGTCGTGGCGAATGCCTTTGACTGAGATCCGGGCGTTTT
  TGACTGGCTTGCCGAATTTATCTGTCACCACACCTTTGATGCCCCGGTGCACCGTCTCCA
  CGAAATTCAGGAGTGACTCCTTGTTGTGCTGCN
  
• Restriction Sites: NotI-NotI
• ACCN: NM_003652
• Insert Size: 2300 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003652.2, NP_003643.2
• RefSeq Size: 2196 bp
• RefSeq ORF: 1926 bp
• Locus ID: 8532
• UniProt ID: Q66K79
• Cytogenetics: 4p16.1
• Domains: FRI, Zn_carbOpept
• Protein Families: Druggable Genome, Protease, Secreted Protein
• Gene Summary: This gene encodes a member of the metallocarboxypeptidase family. This enzyme displays carboxypeptidase activity towards substrates with basic C-terminal residues. It is most active at neutral pH and is inhibited by active site-directed inhibitors of metallocarboxypeptidases. Alternative splicing in the coding region results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (2) lacks an alternate in-frame exon, compared to variant 1, resulting in a shorter protein (isoform 2) that has a shorter N-terminus, compared to isoform 1.