ARHGAP12 (NM_018287) Human Untagged Clone

CAT#: SC125524

ARHGAP12 (untagged)-Human Rho GTPase activating protein 12 (ARHGAP12)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ARHGAP12
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_018287 edited
  ATGAAAATGGCTGACAGAAGTGGGAAGATTATTCCAGGACAAGTGTATATTGAGGTGGAA
  TATGATTATGAATATGAAGCAAAGGACAGAAAGATTGTGATAAAACAAGGGGAGAGGTAC
  ATCTTGGTGAAAAAGACCAATGATGACTGGTGGCAAGTCAAGCCAGATGAAAACTCCAAA
  GCGTTTTATGTGCCAGCCCAGTATGTGAAGGAGGTCACGCGCAAAGCTCTCATGCCACCT
  GTTAAGCAGGTAGCTGGTCTGCCAAATAACTCCACGAAAATAATGCAGAGTTTGCATCTT
  CAGAGATCAACAGAAAATGTGAACAAATTGCCTGAGCTTTCAAGTTTCGGAAAGCCATCG
  TCATCTGTTCAAGGAACAGGTCTTATTCGTGATGCCAATCAGAATTTTGGACCCAGTTAT
  AATCAAGGTCAGACTGTCAACCTAAGCCTGGACCTGACCCATAATAACGGAAAGTTTAAC
  AATGACTCACATTCTCCTAAAGTTTCCAGCCAGAATAGGACACGCTCATTTGGTCATTTT
  CCCGGTCCAGAGTTCTTGGATGTAGAGAAAACTAGCTTCTCCCAGGAACAATCTTGTGAT
  TCCGCAGGAGAAGGCTCTGAAAGAATACATCAAGATTCTGAATCTGGTGATGAACTTAGC
  AGCAGCTCCACTGAACAGATAAGGGCAACCACACCTCCAAATCAAGGAAGGCCAGATTCT
  CCTGTCTATGCTAACCTTCAAGAACTGAAAATATCCCAGTCTGCTCTTCCCCCACTTCCT
  GGGAGCCCGGCAATTCAGATTAATGGAGAATGGGAAACTCATAAAGACAGCTCAGGGCGT
  TGCTATTACTATAACAGAGGGACACAGGAAAGAACTTGGAAACCTCCTCGTTGGACTCGG
  GATGCAAGCATCAGCAAAGGAGATTTCCAAAATCCAGGGGATCAAGAGCTTCTTTCATCG
  GAAGAAAACTACTACAGCACTTCTTACAGCCAGTCAGATAGTCAGTGTGGTTCTCCTCCA
  AGGGGTTGGTCAGAAGAGTTGGATGAACGTGGGCATACCTTATATACCAGTGACTATACT
  AATGAAAAGTGGCTCAAGCATGTTGATGATCAAGGTAGACAATATTACTACAGTGCAGAC
  GGATCTCGGTCAGAATGGGAATTGCCAAAGTATAATGCTTCATCCCAGCAGCAAAGAGAA
  ATAATTAAAAGTAGGAGCCTGGACAGGCGGCTGCAAGAACCAATAGTATTAACAAAGTGG
  AGACATAGCACCATTGTATTGGACACTAATGATAAGGAATCTCCAACTGCCTCAAAACCC
  TGCTTTCCTGAAAATGAGTCTTCTCCCTCCTCACCAAAGCACCAAGATACAGCCAGCAGT
  CCAAAGGATCAAGAGAAATATGGATTATTAAATGTAACAAAAATTGCTGAAAATGGGAAA
  AAGGTTCGAAAGAACTGGTTGTCTTCTTGGGCGGTGTTGCAGGGTTCATCTTTACTTTTT
  ACCAAAACTCAAGGAAGTAGCACAAGTTGGTTTGGCAGTAATCAGTCCAAACCAGAGTTC
  ACAGTGGACCTCAAGGGGGCAACAATTGAGATGGCTTCAAAGGATAAATCCAGCAAAAAG
  AATGTATTTGAGCTGAAAACTCGTCAAGGAACAGAACTGCTAATTCAGTCTGACAATGAC
  ACTGTTATTAATGATTGGTTTAAAGTTCTTAGTAGTACAATCAATAATCAGGCAGTAGAA
  ACTGATGAAGGAATTGAAGAGGAGATACCGGATTCACCAGGAATAGAAAAGCATGATAAA
  GAAAAGGAACAAAAGGATCCCAAAAAGCTTCGTTCCTTTAAAGTATCTAGCATAGATTCT
  TCAGAACAGAAAAAAACCAAGAAAAACTTAAAGAAGTTTCTTACACGACGCCCCACTTTG
  CAAGCTGTTCGTGAAAAAGGTTATATTAAAGATCAGGTATTTGGATCCAATCTCGCTAAT
  CTGTGTCAGAGAGAGAATGGCACAGTACCAAAGTTTGTGAAGTTATGTATTGAACATGTT
  GAAGAACATGGTTTGGATATTGATGGGATATACAGAGTAAGTGGCAACCTCGCAGTGATC
  CAGAAACTAAGGTTTGCAGTCAATCATGATGAGAAATTGGACTTGAATGACAGTAAATGG
  GAAGATATTCATGTCATTACTGGAGCCCTCAAAATGTTTTTTCGAGAATTACCAGAACCT
  CTTTTTACATTTAATCATTTTAATGATTTTGTTAATGCAATTAAGCAAGAACCAAGACAG
  CGAGTCGCTGCTGTTAAGGACCTAATCAGACAGTTGCCAAAGCCAAACCAAGACACAATG
  CAGATTCTTTTCCGACATCTCACAAGAGTTATAGAAAATGGAGAGAAAAATCGAATGACC
  TATCAGAGTATAGCAATTGTTTTTGGTCCCACTCTATTAAAACCAGAAAAAGAGACTGGT
  AATATAGCAGTTCATACTGTGTACCAGAATCAGATTGTAGAATTAATTCTTCTGGAACTG
  AGTTCCATCTTCGGACGTTGA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_018287 unedited
  GAATTCGGCACCAGCGGGCCGGCCGCGGCCGTAGCCGTGTGAACGCTCTTCGGGCTCGCG
  TCGTCGACCCGCAGCCGCGGGGCGGACTAAGAAGGGAGCCGCCTGCTGCGAGGCCGCCGG
  GCTCCCGGTTTAAATGTGATACTGGTGTAGATCTCCTTGTTCTAAGCTATATTCATCCAA
  CAAGCCATAAAGCCATAATGTGGTATAACATCCTTTTTGAGAGGTGAATATTATTGAATG
  AAAATGGCTGACAGAAGTGGGAAGATTATTCCAGGACAAGTGTATATTGAGGTGGAATAT
  GATTATGAATATGAAGCAAAGGACAGAAAGATTGTGATAAAACAAGGGGAGAGGTACATC
  TTGGTGAAAAAGACCAATGATGACTGGTGGCAAGTCAAGCCAGATGAAAACTCCAAAGCG
  TTTTATGTGCCAGCCCAGTATGTGAAGGAGGTCACGCGCAAAGCTCTCATGCCACCTGTT
  AAGCAGGTAGCTGGTCTGCCAAATAACTCCACGAAAATAATGCAGAGTTTGCATCTTCAG
  AGATCAACAGANAATGTGAACAAATTGCCTGAGCTTTCAAGTTTCGGAAAGCCATCGTCA
  TCTGTTCAGGGACAGGTCTTATTCGTGATGCCAATCAGAATTTTGGACCCAGTTATAATC
  AAGGTCAGACTGTCAACCTAAGCCTGGACCTGACCCATANTNNACGAAGTTTACAATGAC
  TCACATTCTCCTAAAGTTTCAGCCAGATANGACACGCTA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_018287
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_018287.4, NP_060757.4
• RefSeq Size: 4173 bp
• RefSeq ORF: 2541 bp
• Locus ID: 94134
• UniProt ID: Q8IWW6
• Cytogenetics: 10p11.22
• Domains: RhoGAP, WW, SH3, PH
• Gene Summary: This gene encodes a member of a large family of proteins that activate Rho-type guanosine triphosphate (GTP) metabolizing enzymes. The encoded protein may be involved in suppressing tumor formation by regulating cell invasion and adhesion. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jul 2012]
  Transcript Variant: This variant (1, also known as ARHGAP12b) represents the longest transcript and encodes the longest isoform (1).