PMP22 (NM_153322) Human Untagged Clone

CAT#: SC124315

PMP22 (untagged)-Human peripheral myelin protein 22 (PMP22), transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PMP22
• Synonyms: CIDP; CMT1A; CMT1E; DSS; GAS-3; GAS3; HMSNIA; HNPP; Sp110
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC124315 sequence for NM_153322 edited (data generated by NextGen Sequencing)
  ATGCTCCTCCTGTTGCTGAGTATCATCGTCCTCCACGTCGCGGTGCTGGTGCTGCTGTTC
  GTCTCCACGATCGTCAGCCAATGGATCGTGGGCAATGGACACGCAACTGATCTCTGGCAG
  AACTGTAGCACCTCTTCCTCAGGAAATGTCCACCACTGTTTCTCATCATCACCAAACGAA
  TGGCTGCAGTCTGTCCAGGCCACCATGATCCTGTCGATCATCTTCAGCATTCTGTCTCTG
  TTCCTGTTCTTCTGCCAACTCTTCACCCTCACCAAGGGGGGCAGGTTTTACATCACTGGA
  ATCTTCCAAATTCTTGCTGGTCTGTGCGTGATGAGTGCTGCGGCCATCTACACGGTGAGG
  CACCCGGAGTGGCATCTCAACTCGGATTACTCCTACGGTTTCGCCTACATCCTGGCCTGG
  GTGGCCTTCCCCCTGGCCCTTCTCAGCGGTGTCATCTATGTGATCTTGCGGAAACGCGAA
  TGA
  
  Clone variation with respect to NM_153322.1
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_153322 unedited
  CGACTTTCTTAGGGCGGCCGCGAATTCGGCACGAGGGGAACATCTCGGGGAGCCTGGTTG
  GAAGCTGCAGGCTTAGTCTGTCGGCTGCGGGTCTCTGACTGCCCTGTGGGGAGGGTCTTG
  CCTTAACATCCCTTGCATTTGGCTGCAAAGAAATCTGCTTGGAAGAAGGGGTTACGCTGT
  TTGGCCGGGCAGAAACTCCGCTGAGCAGAACTTGCCGCCAGAATGCTCCTCCTGTTGCTG
  AGTATCATCGTCCTCCACGTCGCGGTGCTGGTGCTGCTGTTCGTCTCCACGATCGTCAGC
  CAATGGATCGTGGGCAATGGACACGCAACTGATCTCTGGCAGAACTGTAGCACCTCTTCC
  TCAGGAAATGTCCACCACTGTTTCTCATCATCACCAAACGAATGGCTGCAGTCTGTCCAG
  GCCACCATGATCCTGTCGATCATCTTCAGCATTCTGTCTCTGTTCCTGTTCTTCTGCCAA
  CTCTTCACCCTCACCAAGGGGNGGCAGGTTTTACATCACTGGAATCTTCCAAATTCTTGC
  TGGTCTGTGCGTGATGAGTGCTGCGGCCATCTACACGGTGAGGCACNCCGGAGTGGCATC
  TCAACTCGGATTACTCCTACGGTTTCGCCTACATCCTGGCCTAGGGTGGCCTTCCCCCTG
  GCCCTTCTCAGCGTGTCATCTATGTGATCTTGCGGAAACGCGAATGAGCCCCAGACGGTC
  TGTCTGAGCTCTGAGCGCTAATGGGGAAGGNNGGGGAGGGGGAACCNNANNGCGCCAAGA
  ATAAGAGCTAGCCCATATCCCAACTCAACCAACCAACAGAAGAGGGGGGGGGGGGGGCCC
  CCTTCCCTTTAAACCCTTTTCTCTCGCCCCGTGCGCGCCTTGGTGCCTCGCCCCTGCGGC
  CCCACCCGCGCCCCACTCCTCCCCCTATCTTTCTTCTAGCATCCCCCCCCCTCCTTCCTC
  TCTCCTCATTCCCCCCTCTA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_153322
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_153322.1, NP_696997.1
• RefSeq Size: 1669 bp
• RefSeq ORF: 483 bp
• Locus ID: 5376
• UniProt ID: Q01453
• Cytogenetics: 17p12
• Protein Families: Transmembrane
• Gene Summary: This gene encodes an integral membrane protein that is a major component of myelin in the peripheral nervous system. Studies suggest two alternately used promoters drive tissue-specific expression. Various mutations of this gene are causes of Charcot-Marie-Tooth disease Type IA, Dejerine-Sottas syndrome, and hereditary neuropathy with liability to pressure palsies. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]
  Transcript Variant: This variant (3) differs in the 5' UTR, compared to variant 1. Variants 1-5 encode the same isoform (1).