CTRB1 (NM_001906) Human Untagged Clone

CAT#: SC122612

CTRB1 (untagged)-Human chymotrypsinogen B1 (CTRB1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CTRB1
• Synonyms: CTRB
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_001906 edited
  GGGACCGGCAGACAGGCGTCCTACACCCCTGCCAGCGGCACCATGGCTTTCCTCTGGCTC
  CTCTCCTGCTGGGCCCTCCTGGGTACCACCTTCGGCTGCGGGGTCCCCGCCATCCACCCT
  GTGCTCAGCGGCCTGTCCAGGATCGTGAATGGGGAGGACGCCGTCCCCGGCTCCTGGCCC
  TGGCAGGTGTCCCTGCAGGACAAAACCGGCTTCCACTTCTGCGGGGGCTCCCTCATCAGC
  GAGGACTGGGTGGTCACCGCTGCCCACTGCGGGGTCAGGACCTCCGACGTGGTCGTGGCT
  GGGGAGTTTGACCAGGGCTCTGACGAGGAGAACATCCAGGTCCTGAAGATCGCCAAGGTC
  TTCAAGAACCCCAAGTTCAGCATTCTGACCGTGAACAATGACATCACCCTGCTGAAGCTG
  GCCACACCTGCCCGCTTCTCCCAGACAGTGTCCGCCGTGTGCCTGCCCAGCGCCGACGAC
  GACTTCCCCGCGGGGACACTGTGTGCCACCACAGGCTGGGGCAAGACCAAGTACAACGCC
  AACAAGACCCCTGACAAGCTGCAGCAGGCAGCCCTGCCCCTCCTGTCCAATGCCGAATGC
  AAGAAGTCCTGGGGCAGGAGGATCACCGACGTGATGATCTGTGCCGGGGCCAGTGGCGTC
  TCCTCCTGCATGGGCGACTCTGGCGGTCCCCTGGTCTGCCAAAAGGATGGAGCCTGGACC
  CTGGTGGGCATTGTGTCCTGGGGCAGCGACACCTGCTCCACCTCCAGCCCTGGCGTGTAC
  GCCCGTGTCACCAAGCTCATACCTTGGGTGCAGAAGATCCTGGCTGCCAACTGAGCCCGC
  GGCTCCCTCCGACCCTGCTCCCCACAGAGCCTCAGTAAACCCATGAAAAAAAAAAAAAAA
  AAAAAAAAAAAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001906 unedited
  TTCANCATTTGTAATACGACTTACTATAGGGCGGCACGCGAATTCGCCATTACGGCCGGG
  GGGGACCGGCAGACAGGCGTCCTACACCCCTGCCAGCGGCACCATGGCTTTCCTCTGGCT
  CCTCTCCTGCTGGGCCCTCCTGGGTACCACCTTCGGCTGCGGGGTCCCCGCCATCCACCC
  TGTGCTCAGCGGCCTGTCCAGGATCGTGAATGGGGAGGACGCCGTCCCCGGCTCCTGGCC
  CTGGCAGGTGTCCCTGCAGGACAAAACCGGCTTCCACTTCTGCGGGGGCTCCCTCATCAG
  CGAGGACTGGGTGGTCACCGCTGCCCACTGCGGGGTCAGGACCTCCGACGTGGTCGTGGC
  TGGGGAGTTTGACCAGGGCTCTGACGAGGAGAACATCCAGGTCCTGAAGATCGCCAAGGT
  CTTCAAGAACCCCAAGTTCAGCATTCTGACCGTGAACAATGACATCACCCTGCTGAAGCT
  GGCCACACCTGCCCGCTTCTCCCAGACAGTGTCCGCCGTGTGCCTGCCCAGCGCCGACGA
  CGACTTCCCCGCGGAGACACTGTGTGCCACCACAGGCTGGGGCAAGACCAAGTACAACGC
  CAACAAGACCCCTGACAAGCTGCAGCAAGCAGCCCTGCCCCTCCTGTCCAATGCCGAATG
  CAAGAAGTCCTGGGGCANNGAGATCACCGACGTGATGATCTGTGCCGAGGCCAGTGGCGT
  CTCCTCCTGCATGAGCGACTCTGGCAGTCCCCTGGTCTGCCAAAAGATGGAGCCTGNACC
  CTGGTGGGCATTGTGTCCTGGGGCAGCGACACCTGCTTCACCTNCAGCCCTGACGTGTAC
  GCCCGTGTCACCAGCTCATACCTTGGGTGCAGAAGATACTGGCTGGCACTGAGCCCGAGC
  TNCCTCGGACCTGCTCCAACGAGCCTAATAACCCC
  
• Restriction Sites: Please inquire
• ACCN: NM_001906
• Insert Size: 915 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001906.2, NP_001897.2
• RefSeq Size: 872 bp
• RefSeq ORF: 792 bp
• Locus ID: 1504
• UniProt ID: P17538
• Cytogenetics: 16q23.1
• Protein Families: Druggable Genome, Protease, Secreted Protein, Transmembrane
• Gene Summary: This gene encodes a member of the serine protease family of enzymes and forms a principal precursor of the pancreatic proteolytic enzymes. The encoded preproprotein is synthesized in the acinar cells of the pancreas and secreted into the small intestine where it undergoes proteolytic activation to generate a functional enzyme. This CTRB1 gene is located head-to-head with the related CTRB2 gene. Some human populations have an alternate haplotype which inverts a 16.6 Kb region containing portions of intron 1, exon 1, and the upstream sequence of the CTRB1 and CTRB2 genes. In this inversion haplotype exon 1 and flanking sequence is swapped in CTRB1 and CTRB2. This inversion is associated with differential gene expression and increased risk for chronic pancreatitis. The GRCh38 assembly represents the minor allele for SNP rs8048956 of the CTRB1 gene. SNP rs8048956 in intron 1 of the CTRB2 gene is diagnostic for this inversion. This CTRB1 gene encodes distinct isoforms, some or all of which may undergo similar processing to generate the mature protein. [provided by RefSeq, Jan 2021]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer protein (isoform 1).