ELMO1 (NM_130442) Human Untagged Clone

CAT#: SC120554

ELMO1 (untagged)-Human engulfment and cell motility 1 (ELMO1), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ELMO1
• Synonyms: CED-12; CED12; ELMO-1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC120554 sequence for NM_130442 edited (data generated by NextGen Sequencing)
  ATGCAGGTGGTGAAGGAGCAGGTTATGAGAGCACTTACAACCAAGCCTAGCTCCCTGGAC
  CAGTTCAAGAGCAAACTGCAGAACCTGAGCTACACTGAGATCCTGAAAATCCGCCAGTCC
  GAGAGGATGAACCAGGAAGATTTCCAGTCCCGCCCGATTTTGGAACTAAAGGAGAAGATT
  CAGCCAGAAATCTTAGAGCTGATCAAACAGCAACGCCTGAACCGCCTTGTGGAAGGGACC
  TGCTTTAGGAAACTCAATGCCCGGCGGAGGCAAGACAAGTTTTGGTATTGTCGGCTTTCG
  CCAAATCACAAAGTCCTGCATTACGGAGACTTAGAAGAGAGTCCTCAGGGAGAAGTGCCC
  CACGATTCCTTGCAGGACAAACTGCCGGTGGCAGATATCAAAGCCGTGGTGACGGGAAAG
  GACTGCCCTCATATGAAAGAGAAAGGTGCCCTTAAACAAAACAAGGAGGTGCTTGAACTC
  GCTTTCTCCATCTTGTATGACTCAAACTGCCAACTGAACTTCATCGCTCCTGACAAGCAT
  GAGTACTGTATCTGGACGGATGGACTGAATGCGCTACTCGGGAAGGACATGATGAGCGAC
  CTGACGCGGAATGACCTGGACACCCTGCTCAGCATGGAAATCAAGCTCCGCCTCCTGGAC
  CTGGAAAACATCCAGATCCCTGACGCACCTCCGCCGATTCCCAAGGAGCCCAGCAACTAT
  GACTTCGTCTATGACTGTAACTGA
  
  Clone variation with respect to NM_130442.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_130442 unedited
  GGGGTTCGNATTTGTATACGACTCCTATAGGCGGCCGCGNAATTCGCACGAGGGCGGGTC
  TCTGTTCACATGNGCTCAACTGGNAAACCTGTTTCATGAACAAGCTTACTCAGGAACCAT
  CTGGTGGTATTCCAGCACATTGTTCTTCAGGGGGACGACTCTAAGTCGCTTTGTGGTGGC
  AGCAGCTTAGAATCAGTATTTGTGGTTGGGAAAGATGGACTTACGGGAGCTTGGTAATGC
  AGGTGGTGAAGGAGCAGGTTATGAGAGCACTTACAACCAAGCCTAGCTCCCTGGACCAGT
  TCAAGAGCAAACTGCAGAACCTGAGCTACACTGAGATCCTGAAAATCCGCCAGTCCGAGA
  GGATGAACCAGGAAGATTTCCAGTCCCGCCCGATTTTGGAACTAAAGGAGAAGATTCAGC
  CAGAAATCTTAGAGCTGATCAAACAGCAACGCCTGAACCGCCTTGTGGAAGGGACCTGCT
  TTAGGAAACTCAATGCCCGGCGGAGGCAAGACAAGTTTTGGTATTGTCGGCTTTCGCCAA
  ATCACAAAGTCCTGCATTACGGAGACTTAGAAGAGAGTCCTCAGGGAGAAGTGCCCCACG
  ATTCCTTGCAGGACAAACTGCCGGTGGCAGATATCAAAGCCGTGGTGACGGGAAAGGACT
  GCCCTCATATGAAAGAGAAAGGTGCCCTTAAACAAACAAGGAGGTGCTTGAACTCGCTNT
  CTCCATCTTGTATGACTCAAACTGCCAACTGAACTTCATCGCTCCTGACAGCATGAGTAC
  TGTATCTGGACGGATGGACTGAATGCGCTNACTCGGAAAGACATGATGAGCGACCCTGAC
  GCGAATGACCTGNACACCCTGCTCAGCATGGAAATCAAGCTCCGCCTNCTGGNACCTGGA
  AACATA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_130442 unedited
  ACCGCGGCCGCAATCTANAGTCGAGTTTTTTTTTTTTTTTTTTTCATCACAATCCATTTT
  ATTCCACAGCATGTTTAAGGGAATGTTATTTTCAGGAAGATGTTTGGGATTAAAATAATT
  AAAAAAAACTGGAAATTCACACATTTATGTATCTCTTCTTTTCATTAAATATATATCTGT
  TTGGCAAAAAATGTTAGACTCTTTTGTACAAAAACAAAGCAAAAAAAAAAAAAAAAACTA
  ACCCAAAACTCTTGCAAATTGTAAATACCAGTAATAATTACAATGATGTAAACTTGGATG
  TCTGACAGCTTGACCATCCTGTGGAACTTGAGTTGCAGCAGGACCTCCCAGGAAAAGGTA
  ACACCAAACGCTTGGATAGCAACAGTCCGTCCTAAAGGCATTTAGGTTTAGTCTATTAAA
  GATGGAGCGAGACTACTGATTTCACCATTTGTGCTTTCAGCTAAAAGGCTGGGACCGTGG
  GTGGAAATGAAGAAGGCAGTTCTGGCCTGGGGCAGGGAGAGGAAACCCCAGACTAGCTGG
  TGAGGAGGGAAGATTTGTCACGATCACAGTGCAGACGCAAAGTGGGCATGGGACCTGTGG
  ACTGTTTGGGAAATTTATGCTAGGGAGGATCACGAGGAGGCTGCGGCTGCTGCTCTTGGG
  ACCAAAGAGGAACAGAGCAGGGCAAAAACAGGGAGAGAGGGTGGAGGAGATTTGAGCGCA
  GATCTTAATTCAAGTGTAACTGGGTGTGCGNAGAGATNCTANNAAAGAGGGAAAAGAGGA
  TGTGGGGGTTTGGTTCTTCTCATCAAATAGCTAGGGCCTTTTCCTTCAGACGGGGCCTTG
  GGGCACTGCCCTGGGTCGGNTGGGCAAATTTTTGGGCANTCTGGGGCTGCTGGCTTTCCT
  GGCCAAGGGAAGAAGGGCCTGCCTGCAAAGGCTAG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_130442
• Insert Size: 2090 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_130442.1, NP_569709.1
• RefSeq Size: 2162 bp
• RefSeq ORF: 744 bp
• Locus ID: 9844
• UniProt ID: Q92556
• Cytogenetics: 7p14.2-p14.1
• Protein Pathways: Chemokine signaling pathway
• Gene Summary: This gene encodes a member of the engulfment and cell motility protein family. These proteins interact with dedicator of cytokinesis proteins to promote phagocytosis and cell migration. Increased expression of this gene and dedicator of cytokinesis 1 may promote glioma cell invasion, and single nucleotide polymorphisms in this gene may be associated with diabetic nephropathy. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2013]
  Transcript Variant: This variant (2) represents use of an alternate promoter and differs in the 5' UTR and 5' coding region, compared to variant 1. These differences cause translation initiation at a downstream AUG and result in an isoform (2) with a shorter N-terminus, compared to isoform 1. Both variants 2 and 3 encode the same isoform (2). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.