Fbx32 (FBXO32) (NM_058229) Human Untagged Clone

CAT#: SC120247

FBXO32 (untagged)-Human F-box protein 32 (FBXO32), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Fbx32
• Synonyms: Fbx32; MAFbx
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC120247 sequence for NM_058229 edited (data generated by NextGen Sequencing)
  ATGCCATTCCTCGGGCAGGACTGGCGGTCCCCCGGGCAGAACTGGGTGAAGACGGCCGAC
  GGCTGGAAGCGCTTCCTGGATGAGAAGAGCGGCAGTTTCGTGAGCGACCTCAGCAGTTAC
  TGCAACAAGGAGGTATACAATAAGGAGAATCTTTTCAACAGCCTGAACTATGATGTTGCA
  GCCAAGAAGAGAAAGAAGGACATGCTGAATAGCAAAACCAAAACTCAGTATTTCCACCAA
  GAAAAATGGATCTATGTTCACAAAGGAAGTACTAAAGAGCGCCATGGATATTGCACCCTG
  GGGGAAGCTTTCAACAGACTGGACTTCTCAACTGCCATTCTGGATTCCAGAAGATTTAAC
  TACGTGGTCCGGCTGTTGGAGCTGATAGCAAAGTCACAGCTCACATCCCTGAGTGGCATC
  GCCCAAAAGAACTTCATGAATATTTTGGAAAAAGTGGTACTGAAAGTCCTTGAAGACCAG
  CAAAACATTAGACTAATAAGGGAACTACTCCAGACCCTCTACACATCCTTATGTACACTG
  GTCCAAAGAGTCGGCAAGTCTGTGCTGGTCGGGAACATTAACATGTGGGTGTATCGGATG
  GAGACGATTCTCCACTGGCAGCAGCAGCTGAACAACATTCAGATCACCAGGCCTGCCTTC
  AAAGGCCTCACCTTCACTGACCTGCCTTTGTGCCTACAACTGAACATCATGCAGAGGCTG
  AGCGACGGGCGGGACCTGGTCAGCCTGGGCCAGGCTGCCCCCGACCTGCACGTGCTCAGC
  GAAGACCGGCTGCTGTGGAAGAAACTCTGCCAGTACCACTTCTCCGAGCGGCAGATCCGC
  AAACGATTAATTCTGTCAGACAAAGGGCAGCTGGATTGGAAGAAGATGTATTTCAAACTT
  GTCCGATGTTACCCAAGGAAAGAGCAGTATGGAGATACCCTTCAGCTCTGCAAACACTGT
  CACATCCTTTCCTGGAAGGGCACTGACCATCCGTGCACTGCCAATAACCCAGAGAGCTGC
  TCCGTTTCACTTTCACCCCAGGACTTTATCAACTTGTTCAAGTTCTGA
  
  Clone variation with respect to NM_058229.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_058229 unedited
  GATTTTGTAATACGACTCACTATAGGGCGGCCGCGCTGCAACGCTTGAGATCCTCTCCGC
  GCCCGCCACCCCGCAGGGTGCCCCGCGCCGTTCCCGCCGCCCCGCCGCCCCCGTCGCGGG
  CCCCTGCACCCCGAGCATCCGCCCCGGGTGGCACGTCCCCGAGCCCACCAGGCCGGCCCC
  GTCTCCCCATCCGTCTAGTCCGCTCGCGGTGCCATGCCATTCCTCGGGCAGGACTGGCGG
  TCCCCCGGGCAGAACTGGGTGAAGACGGCCGACGGCTGGAAGCGCTTCCTGGATGAGAAG
  AGCGGCAGTTTCGTGAGCGACCTCAGCAGTTACTGCAACAAGGAGGTATACAATAAGGAG
  AATCTTTTCAACAGCCTGAACTATGATGTTGCAGCCAAGAAGAGAAAGAAGGACATGCTG
  AATAGCAAAACCAAAACTCAGTATTTCCACCAAGAAAAATGGATCTATGTTCACAAAGGA
  AGTACTAAAGAGCGCCATGGATATTGCACCCTGGGGGAAGCTTTCAACAGACTGGACTTC
  TCAACTGCCATTCTGGATTCCAGAAGATTTAACTACGTGGTCCGGCTGTTGGAGCTGATA
  GCAAAGTCACAGCTCACATCCCTGAGTGGCATCGCCCAAAAGAACTTCATGAATATTTTG
  GAAAAAGTGGTACTGAAAGTCCTTGAAGACCAGCAAAACATTAGACCTAATAGGGAACTA
  CTCCAGACCCTCTACACATNCTTATGTACACTGGTCCAAAGAGTCGGNCAGTCTGTGCTG
  GTCGGGAACATTACATGGNGGGTGTATCGGATGGAGACAATTCTCACTGGCAGCAGCAGC
  TGAACACATTCAGATCACCAGCCTGGCTTCAAGGCCTCACCTTCACTGACCTGNCTTNNG
  TGGCTACACTGACTCATGCANAGGCTGACGACGGNCGGNACCTGGTCAGNCTGGGCCAGC
  TGNCCCCGACTGCCGTGCTTANNGAGACCGCTGNTTG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_058229 unedited
  NGGCCTATTATGTACCGCGCCGCATTCTANGATCGATTTTTTTTTTTTTTTTTTTTGTTA
  AAAAAAAATTTATTTACAGTATTTTGCTTTTCCATACCAATTCTAGTGAGAAGTTCACAT
  TCTTAAATTCCCAGTCAGCAACTGCATTTCTCCCCTCCAATGTCCTCTCCATGACTTATC
  TCTGAAGTTTCGAGCCAATGTTTAAAATGTACACTATTTACAAATGAAGTGTCCAAATGC
  CATATTCCCAGCTCTCCAGTCAGCAGGGGGACCCTTCTGAAGTGTTGTCATGTGCTGGGA
  TTCAGAACTTGAACAAGTTGATAAAGTCCTGGGGTGAAAGTGAAACGGAGCAGCTCTCTG
  GGTTATTGGCAGTGCACGGATGGTCAGTGCCCTTCCAGGAAAGGATGTGACAGTGTTTGC
  AGAGCTGAAGGGTATCTCCATACTGCTCTTTCCTTGGGTAACATCGGACAAGTTTGAAAT
  ACATCTTCTTCCAATCCAGCTGCCCTTTGTCTGACAGAATTAATCGTTTGCGGATCTGCC
  GCTCGGAGAAGTGGTACTGGCAGAGTTTCTTCCACAGCAGCCGGTCTTCGCTGAGCACGT
  GCAGGTCGGGGGCAGCCTGGCCCATGCTGACCAGGTCCCGCCCGTCGCTCAGCCTCTGCA
  TGATGTTCAGTTGTAAGCACAAAGGCAGGTCAGTGAAAGTGATGCCTTTGAAGGCAGGCC
  TGGTGATCTGAATGTTGTTCAACTGCTGCTGCCAGTGGAGAATTGTCTCCATCCGATACA
  CCCACATGTTAATGTTTCCCGACCAGCACAGACTTGCCGACTCTTTGGACCAGTGTACAT
  AAGATGTGGTAAAGGGTCTGGAATAA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_058229
• Insert Size: 1500 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_058229.2, NP_478136.1
• RefSeq Size: 1530 bp
• RefSeq ORF: 1068 bp
• Locus ID: 114907
• UniProt ID: Q969P5
• Cytogenetics: 8q24.13
• Domains: F-box
• Gene Summary: This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of the ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class and contains an F-box domain. This protein is highly expressed during muscle atrophy, whereas mice deficient in this gene were found to be resistant to atrophy. This protein is thus a potential drug target for the treatment of muscle atrophy. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jun 2011]
  Transcript Variant: This variant (1) has a longer and alternate 5' terminal sequence containing an in-frame translation start codon, as compared to variant 2. It encodes a longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.