MRPL53 (NM_053050) Human Untagged Clone

CAT#: SC120204

MRPL53 (untagged)-Human mitochondrial ribosomal protein L53 (MRPL53), nuclear gene encoding mitochondrial protein

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MRPL53
• Synonyms: L53MT
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC120204 sequence for NM_053050 edited (data generated by NextGen Sequencing)
  ATGGCAGCTGCCTTGGCTCGGCTTGGTCTGCGGCCTGTCAAACAGGTTCGGGTTCAGTTC
  TGTCCCTTCGAGAAAAACGTGGAATCGACGAGGACCTTCCTGCAGACGGTGAGCAGTGAG
  AAGGTCCGCTCCACTAATCTCAACTGCTCAGTGATTGCGGACGTGAGGCATGACGGCTCC
  GAGCCCTGCGTGGACGTGCTGTTCGGAGACGGGCATCGCCTGATTATGCGCGGCGCTCAT
  CTCACCGCTCTGGAAATGCTCACCGCCTTCGCCTCCCACATCCGGGCCAGGGACGCGGCG
  GGCAGCGGGGACAAGCCGGGCGCTGATACTGGTCGCTGA
  
  Clone variation with respect to NM_053050.4
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_053050 unedited
  AGGATATTTGTATACGACTCACTTATAGGGCGGCCGCGAATTCGCACGAGGCATGGCAGC
  TGCCTTGGCTCGGCTTGGTCTGCGGCCTGTCAAACAGGTTCGGGTTCAGTTCTGTCCCTT
  CGAGAAAAACGTGGAATCGACGAGGACCTTCCTGCAGACGGTGAGCAGTGAGAAGGTCCG
  CTCCACTAATCTCAACTGCTCAGTGATTGCGGACGTGAGGCATGACGGCTCCGAGCCCTG
  CGTGGACGTGCTGTTCGGAGACGGGCATCGCCTGATTATGCGCGGCGCTCATCTCACCGC
  TCTGGAAATGCTCACCGCCTTCGCCTCCCACATCCGGGCCAGGGACGCGGCGGGCAGCGG
  GGACAAGCCGGGCGCTGATACTGGTCGCTGACAGCGCCAAAGAGACCAACAAGATGATTT
  TAGCGTGGACTAGGACACTTAACCTAAGAAGAGTTTCACTTAATCATTCAAATCACTATC
  TGAAGGGTCACGGAGCGCAAAATAAAGTTTAAAACCCTGCTAAAAAAAAAAAAAAAAAAC
  TCGACTCTAGATTGCGGCCGCGGTCATAGCTGTTTCCTGAACAGATCCCGGGTGGCATCC
  CTGTGACCCCTCCCCAGTGCCTCTCCTGGCCCTGGAAGTTGCCACTCCAGTGCCCACCAG
  CCTTGTCCTAATAAAATTAAGTTGCATCATTTTGTCTGACTAGGTGTCCTTCTATATATT
  ATGGGGTGGAGGGGGGATGTATGGAGCAAGGGGCAAGTTGNGAAGACAACCTGTAGGGCC
  TGCGGGGTCTATTGGGACCAAGCTGGATGCAGGGCACAATCTTGGCCACTGAATCTCCGC
  CTCTGGTTCAGCGATCTCCTGCCTACCTCCGAGTGTTGGATTCAGCTTGCTGACAGGTCA
  CTATTTTGTTTTTGGAGAAACGGTTCACCTATGCCAGCTGCCN
  
• Restriction Sites: NotI-NotI
• ACCN: NM_053050
• Insert Size: 1460 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_053050.2, NP_444278.1
• RefSeq Size: 632 bp
• RefSeq ORF: 339 bp
• Locus ID: 116540
• UniProt ID: Q96EL3
• Cytogenetics: 2p13.1
• Gene Summary: Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. A pseudogene corresponding to this gene is found on chromosome 1p. [provided by RefSeq, Jul 2008]