IFT43 (NM_052873) Human Untagged Clone

CAT#: SC120155

IFT43 (untagged)-Human chromosome 14 open reading frame 179 (C14orf179), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: IFT43
• Synonyms: C14orf179; CED3; RP81; SRTD18
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_052873, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGAGGATTTGCTCGACTTGGACGAGGAGCTTCGCTACAGCTTGGCTACCTCCAGGGCCAAGATGGGTC
  GCCGAGCTCAACAGGAGTCAGCGCAGGCCGAGAATCACCTCAATGGCAAGAATTCCTCTTTGACTCTGAC
  TGGAGAGACTTCCTCTGCTAAATTACCTCGCTGCCGACAGGGAGGCTGGGCAGGTGATTCCGTGAAGGCT
  TCGAACGGTACCCAAACAGGCAAACAACAGCTGGATCTGAACGCATGCTATCACAAAACGCATCACAGAG
  ATTTGGGGCTGGCTTCATTGGAAGAGGCAGATATTCCTATCATTCCGGATCTGGAGGAAGTACAGGAAGA
  AGACTTTGTTTTGCAGGTGGCAGCCCCTCCCAGCATCCAGATAAAGCGGGTGATGACCTACCGTGACCTG
  GACAATGACCTCATGAAGTACTCAGCCATTCAGACACTGGATGGGGAGATCGACCTGAAACTCCTCACCA
  AAGTGCTCGCGCCGGAGCACGAAGTCCGGGAGGATGATGTCGGCTGGGACTGGGACCATCTGTTCACTGA
  GGTGTCCTCAGAGGTCCTCACTGAGTGGGACCCACTGCAGACGGAGAAGGAGGACCCTGCGGGGCAGGCC
  AGGCACACCTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_052873 unedited
  GGGTGCANAATTTGTTATACGACTCCACTATAGGGCGGCCGCGATTCGGCACGAGGCGGA
  GATGGAGGATTTGCTCGACTTGGACGAGGAGCTTCGCTACAGCTTGGCTACCTCCAGGGC
  CAAGATGGGTCGCCGAGCTCAACAGGAGTCAGCGCAGGCCGAGAATCACCTCAATGGCAA
  GAATTCCTCTTTGACTCTGACTGGAGAGACTTCCTCTGCTAAATTACCTCGCTGCCGACA
  GGGAGGCTGGGCAGGTGATTCCGTGAAGGCTTCGAAGTTTAGGAGGAAGGCTTCTGAAGA
  AATAGAAGATTTCCGCCTCAGACCACAGAGCCTGAATGGATCAGATTATGGAGGAGATAT
  TCCTATCATTCCGGATCTGGAGGAAGTACAGGAAGAAGACTTTGTTTTGCAGGTGGCAGC
  CCCTCCCAGCATCCAGATAAAGCGGGTGATGACCTACCGTGACCTGGACAATGACCTCAT
  GAAGTACTCAGCCATTCAGACACTGGATGGGGAGATCGACCTGAAACTCCTCACCAAAGT
  GCTCGCGCCGGAGCACGAAGTCCGGGAGGATGATGTCGGCTGGGACTGGGACCATCTGTT
  CACTGAGGTGTCCTCAGAGGTCCTCACTGAGTGGGACCCACTGCAGACGGAGAAGGAGGA
  CCCTGCGGTGCAGGCCAGGCACACCTGAGCCCCGTCACCATGCTCTAGACATGAAGAAAT
  GCCATGAGCTTAAAGCTAAAAAAGCTTGTAAGCAGCTCCGAATTTTTACCTGGGATATTT
  TGTTATAAAAATATCTATATTCAGTCACCCCATTGGATAATCCAATGGCATAAATTGCTT
  ATTCTGGCCCTCTCAAAAAAAAAAAAAAACTCCACTCTAAATTGCGGCCGCGTTCTAACC
  TG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_052873 unedited
  GGGTTTCGCTTTGGACCCGCGGCCGCAANCTAGGATCGAGTTTTTTTTTTTTTTTTTTGA
  GGATGGCACAGAATAAGCAATTTATTGCAATTGAATTATCCAATGTGGTTGACTGAATAT
  AAATATTTTTATTACAAAATATTCCAGGTAAAAATTCGGAGCTGCTTACAAGCTTCTTTA
  GCTTTAAGCTCATTGCATTTCTTCATGTCTAGAGCATGGGTGACGGGCTCAGGTGTGCCT
  GGCCTGCCCCGCAGGGTCCTCCTTCTCCGTCTGCAGTGGGTCCCACTCAGTGAGGACCTC
  TGAGGACACCTCAGTGAACAGATGGTCCCAGTCCCAGCCGACATCATCCTCCCGGACTTC
  GTGCTCCGGCGCGAGCACTTTGGTGAGGAGTTTCAGGTCGATCTCCCCATCCAGTGTCTG
  AATGGCTGAGTACTTCATGAGGTCATTGTCCAGGTCACGGTAGGTCATCACCCGCTTTAT
  CTGGATGCTGGGAGGGGCTGCCACCTGCAAAACAAAGTCTTCTTCCTGTACTTCCTCCAG
  ATCCGGAATGATAGGAATATCTCCTCCATAATCTGATCCATTCAGGCTCTGTGGTCTGAG
  GCGGAAATCTTCTATTTCTTCAGAAGCCTTCCTCCTAAACTTCGAAGCCTTCACGGAATC
  ACCTGCCCAGCCTCCCTGTCGGCAGCGAGGTAATTTAGCAGAGGAAGTCTCTCCAGTCAG
  AGTCAAAGAGGAATTCTTGCCATTGAGGTGATTCTCGGCCTGCGCTGACTCCTGTTGAGC
  TCGGCGACCCCATCTGGCCCTGGAGTAGCCAAGCTGTAGCGAAGCTCCTCGTCCAAGTCG
  AGCAAATCCTCCATCTCCGCCTCGTGCCGAATTCGCGGCCGCCTATAGTGAGTCGTATAC
  CAAATCCTGA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_052873
• Insert Size: 760 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_052873.1, NP_443105.1
• RefSeq Size: 850 bp
• RefSeq ORF: 642 bp
• Locus ID: 112752
• UniProt ID: Q96FT9
• Cytogenetics: 14q24.3
• Gene Summary: This gene encodes a subunit of the intraflagellar transport complex A (IFT-A). IFT-A is a multiprotein complex that plays an important role in cilia assembly and maintenance by mediating retrograde ciliary transport. Mutations in this gene are a cause of cranioectodermal dysplasia-3 (CED3), also known as Sensenbrenner syndrome. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Dec 2011]
  Transcript Variant: This variant (1) encodes the longest isoform (1).