Factor I (CFI) (NM_000204) Human Untagged Clone
Product Images
![](https://cdn.origene.com/img/defaults-img-expression-plasmids.jpg)
Specifications
Product Data | |
Type | Human Untagged Clone |
Tag | Tag Free |
Symbol | Factor I |
Synonyms | AHUS3; ARMD13; C3b-INA; C3BINA; FI; IF; KAF |
Vector | pCMV6-XL4 |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | None |
Sequence Data |
>OriGene ORF within SC120051 sequence for NM_000204 edited (data generated by NextGen Sequencing)
ATGAAGCTTCTTCATGTTTTCCTGTTATTTCTGTGCTTCCACTTAAGGTTTTGCAAGGTC ACTTATACATCTCAAGAGGATCTGGTGGAGAAAAAGTGCTTAGCAAAAAAATATACTCAC CTCTCCTGCGATAAAGTCTTCTGCCAGCCATGGCAGAGATGCATTGAGGGCACCTGTGTT TGTAAACTACCGTATCAGTGCCCAAAGAATGGCACTGCAGTGTGTGCAACTAACAGGAGA AGCTTCCCAACATACTGTCAACAAAAGAGTTTGGAATGTCTTCATCCAGGGACAAAGTTT TTAAATAACGGAACATGCACAGCCGAAGGAAAGTTTAGTGTTTCCTTGAAGCATGGAAAT ACAGATTCAGAGGGAATAGTTGAAGTAAAACTTGTGGACCAAGATAAGACAATGTTCATA TGCAAAAGCAGCTGGAGCATGAGGGAAGCCAACGTGGCCTGCCTTGACCTTGGGTTTCAA CAAGGTGCTGATACTCAAAGAAGGTTTAAGTTGTCTGATCTCTCTATAAATTCCACTGAA TGTCTACATGTGCATTGCCGAGGATTAGAGACCAGTTTGGCTGAATGTACTTTTACTAAG AGAAGAACTATGGGTTACCAGGATTTCGCTGATGTGGTTTGTTATACACAGAAAGCAGAT TCTCCAATGGATGACTTCTTTCAGTGTGTGAATGGGAAATACATTTCTCAGATGAAAGCC TGTGATGGTATCAATGATTGTGGAGACCAAAGTGATGAACTGTGTTGTAAAGCATGCCAA GGCAAAGGCTTCCATTGCAAATCAGGTGTTTGCATTCCAAGCCAGTATCAATGCAATGGT GAGGTGGACTGCATTACAGGGGAAGATGAAGTTGGCTGTGCAGGCTTTGCATCTGTGGCT CAAGAAGAAACAGAAATTTTGACTGCTGACATGGATGCAGAAAGAAGACGGATAAAATCA TTATTACCTAAACTATCTTGTGGAGTTAAAAACAGAATGCACATTCGAAGGAAACGAATT GTGGGAGGAAAGCGAGCACAACTGGGAGACCTCCCATGGCAGGTGGCAATTAAGGATGCC AGTGGAATCACCTGTGGGGGAATTTATATTGGTGGCTGTTGGATTCTGACTGCTGCACAT TGTCTCAGAGCCAGTAAAACTCATCGTTACCAAATATGGACAACAGTAGTAGACTGGATA CACCCCGACCTTAAACGTATAGTAATTGAATACGTGGATAGAATTATTTTCCATGAAAAC TACAATGCAGGCACTTACCAAAATGACATCGCTTTGATTGAAATGAAAAAAGACGGAAAC AAAAAAGATTGTGAGCTGCCTCGTTCCATCCCTGCCTGTGTCCCCTGGTCTCCTTACCTA TTCCAACCTAATGATACATGCATCGTTTCTGGCTGGGGACGAGAAAAAGATAACGAAAGA GTCTTTTCACTTCAGTGGGGTGAAGTTAAACTAATAAGCAACTGCTCTAAGTTTTACGGA AATCGTTTCTATGAAAAAGAAATGGAATGTGCAGGTACATATGATGGTTCCATCGATGCC TGTAAAGGGGACTCTGGAGGCCCCTTAGTCTGTATGGATGCCAACAATGTGACTTATGTC TGGGGTGTTGTGAGTTGGGGGGAAAACTGTGGAAAACCAGAGTTCCCAGGTGTTTACACC AAAGTGGCCAATTATTTTGACTGGATTAGCTACCATGTAGGAAGGCCTTTTATTTCTCAG TACAATGTATAA Clone variation with respect to NM_000204.3 804 g=>a >OriGene 5' read for NM_000204 unedited
NGGGGTCACCATTTGTATACGACTCCTATAGGCGGCCCGCGAATTCGCACCAGNAGAAAA ANACCCCGACACCTCCAACATGAACTTCTTCATGTTTTCCTGTTATTTCTGTGCTTCCAC TTAAGGTTTTGCAAGGTCACTTATACATCTCAAGAGGATCTGGTGGAGAAAAAGTGCTTA GCAAAAAAATATACTCACCTCTCCTGCGATAAAGTCTTCTGCCAGCCATGGCAGAGATGC ATTGAGGGCACCTGTGTTTGTAAACTACCGTATCAGTGCCCAAAGAATGGCACTGCAGTG TGTGCAACTAACAGGAGAAGCTTCCCAACATACTGTCAACAAAAGAGTTTGGAATGTCTT CATCCAGGGACAAAGTTTTTAAATAACGGAACATGCACAGCCGAAGGAAAGTTTAGTGTT TCCTTGAAGCATGGAAATACAGATTCAGAGGGAATAGTTGAAGTAAAACTTGTGGACCAA GATAAGACAATGTTCATATGCAAAAGCAGCTGGAGCATGAGGGAAGCCAACGTGGCCTGC CTTGACCTTGGGTTTCAACAAGGTGCTGATACTCAAAGAAGGTTTAAGTTGTCTGATCTC TCTATAAATTCCACTGAATGTCTACATGTGCATTGCCGAGGATTAGAGACCAGTTTGGCT GAATGTACTTTTACTAAGAGAAGAACTATGGGTTACCAGGATTTCGCTGATGTGGTTTTG TTATACACAGAAAGCAGATTCTCCAATGGATGACTTCTTTCAGTGTGTGAATGGGAAATA CATTTCTCAGATGAAAGCCTGTGATGTTATCAATGATTGTGGAGACCAAATGATGAACTG TGTTGTAAACATGCCAAGGCAAAGCTTCCATTGCAAATAGGGGTTTGCATTCCAGCA >OriGene 3' read for NM_000204 unedited
NTTTAGCTTGNACCCGCGGCCGCATNCTAGGATCGAGTTTTTTTTTTTTTTTTTTTTTTT TATGCTTCACCAAANATATTTATTTGAGAATTATACAACAAAATTCCAATATGGCATAAA CTCTGTGGAGACCTTTAAAAATATCCAATGAGATTTGCTTCATTTTTCCCCCCTAGAGAA TTATTAATTATACCGTTTTATTTCCATTAAATGGAACTCTTGAGAGAAAAAGAATAGAAT GAAGAGAGAGATCACAATTTTATACATTGTACTGAGAAATAAAAGGCCTTCCTACATGGT AGCTAATCCAGTCAAAATAATTGGCCACTTTGGTGTAAACACCTGGGAACTCTGGTTTTC CACAGTTTTCCCCCCAACTCACAACACCCCAGACATAAGTCACATTGTTGGCATCCATAC AGACTAAGGGGCCTCCAGAGTCCCCTTTACAGGCATCGATGGAACCATCATATGTACCTG CACATTCCATTTCTTTTTCATAGAAACGATTTCCGTAAAACTTAGAGCAGTTGCTTATTA GTTTAACTTCACCCCACTGAAGTGAAAAGACTCTTTCGTTATCTTTTTCTCGTCCCCAGC CAGAAACGATGCATGTATCATTAGGTTGGAATAGGTAAGGAGACCAGGGGACACAGGCAG GGATGGAACGAGGCAGCTCACAATCTTTTTTGTTTCCGTCTTTTTTCATTTCAATCAAAG CGATGTCATTTTGGTAAGTGCCTGCATTGTAGTTTTCATGGAAAATAATTCTATCCACGT ATTCAATTACTATACGTTTAAGGTCGGGGTGTATCCAGTCTACTACTGTTGTCCATATTT GGTACGATGAGTTTTACTGGCTCTGAGNACATGTGCAGCAGTCAGAATCCACAGCCACCC ATATAAATCCCCACAGGTGATNCACTGGCATNCTTAATGCCCCTGCATGGAA |
Restriction Sites | NotI-NotI |
ACCN | NM_000204 |
Insert Size | 2260 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_000204.1, NP_000195.1 |
RefSeq Size | 1963 bp |
RefSeq ORF | 1752 bp |
Locus ID | 3426 |
UniProt ID | P05156 |
Cytogenetics | 4q25 |
Domains | SR, Tryp_SPc, ldl_recept_a, FIMAC |
Protein Families | Druggable Genome, Protease, Secreted Protein |
Protein Pathways | Complement and coagulation cascades |
Gene Summary | This gene encodes a serine proteinase that is essential for regulating the complement cascade. The encoded preproprotein is cleaved to produce both heavy and light chains, which are linked by disulfide bonds to form a heterodimeric glycoprotein. This heterodimer can cleave and inactivate the complement components C4b and C3b, and it prevents the assembly of the C3 and C5 convertase enzymes. Defects in this gene cause complement factor I deficiency, an autosomal recessive disease associated with a susceptibility to pyogenic infections. Mutations in this gene have been associated with a predisposition to atypical hemolytic uremic syndrome, a disease characterized by acute renal failure, microangiopathic hemolytic anemia and thrombocytopenia. Primary glomerulonephritis with immune deposits and age-related macular degeneration are other conditions associated with mutations of this gene. [provided by RefSeq, Dec 2015] Transcript Variant: This variant (2) encodes isoform 2. |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC216645 | CFI (Myc-DDK-tagged)-Human complement factor I (CFI) |
USD 543.00 |
|
RC216645L1 | Lenti ORF clone of Human complement factor I (CFI), Myc-DDK-tagged |
USD 843.00 |
|
RC216645L2 | Lenti ORF clone of Human complement factor I (CFI), mGFP tagged |
USD 843.00 |
|
RC216645L3 | Lenti ORF clone of Human complement factor I (CFI), Myc-DDK-tagged |
USD 843.00 |
|
RC216645L4 | Lenti ORF clone of Human complement factor I (CFI), mGFP tagged |
USD 843.00 |
|
RG216645 | CFI (tGFP-tagged) - Human complement factor I (CFI) |
USD 743.00 |
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