PCBD1 (NM_000281) Human Untagged Clone

CAT#: SC120016

PCBD1 (untagged)-Human pterin-4 alpha-carbinolamine dehydratase/dimerization cofactor of hepatocyte nuclear factor 1 alpha (PCBD1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PCBD1
• Synonyms: DCOH; PCBD; PCD; PHS
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_000281 edited
  ATGGCTGGCAAAGCACACAGGCTGAGCGCTGAGGAGAGGGACCAGCTGCTGCCAAACCTG
  AGGGCTGTGGGGTGGAATGAGCTGGAAGGCCGTGATGCCATCTTCAAGCAGTTTCATTTC
  AAAGACTTCAACAGGGCCTTTGGGTTCATGACAAGAGTGGCCCTGCAGGCTGAGAAACTG
  GACCACCATCCTGAATGGTTTAACGTGTACAACAAGGTCCACATCACGCTGAGCACCCAT
  GAGTGTGCCGGCCTTTCAGAACGGGACATAAACCTGGCCAGCTTCATCGAACAAGTAGCA
  GTGTCCATGACATAG
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000281 unedited
  NGGTTCACATATTTGNTATACGACTCACTATTAGGGCGGCCGCGATTCGGCACGAGGGCC
  GCCCGCGCCATGGCTGGCAAAGCACACAGGCTGAGCGCTGAGGAGAGGGACCAGCTGCTG
  CCAAACCTGAGGGCTGTGGGGTGGAATGAGCTGGAAGGCCGTGATGCCATCTTCAAGCAG
  TTTCATTTCAAAGACTTCAACAGGGCCTTTGGGTTCATGACAAGAGTGGCCCTGCAGGCT
  GAGAAACTGGACCACCATCCTGAATGGTTTAACGTGTACAACAAGGTCCACATCACGCTG
  AGCACCCATGAGTGTGCCGGCCTTTCAGAACGGGACATAAACCTGGCCAGCTTCATCGAA
  CAAGTAGCAGTGTCCATGACATAGACCCTGCCCTTCCTCTTTGAATTCTTCCGGGGGAAA
  GGGTGACTGAACTGGGAGTCCAGGGAGGGAGCTGAGGAGCCCTTACCCTCCCACCACTCC
  CCTCCCAAGACCCAGCCGCCGCCGTTGAGGGCTGAGTCCTTGCTGTGGGATGTGCCAGTG
  TCCCCACCAACACCAGGAATTTAGACCTTTTCCCTGCACCACTCTCTTCATCCTGNGGGC
  TCTGTTACACTAATTTGAATAAACTCTCCCCTTTCTTTGCAACTTCCCAGCAACAATAAT
  GATTTTCTTGCCAGGCCGTCTCTTGCTCCCTATTCATTTCCCAGGAAGCTGTGATACAGG
  GTGAAATAAAGTCTTGTCTTAGAAAACCAGACCCTAAACCCCCCACTATGTAATAGAAAC
  ACATGTGGTTTTTATGTCTCANATAAAACTATTATATCACTTGGAAAAAAAAAAAAAAAA
  AACTCGACTCTAGATTGCGGNCCGCGTCATAGCTGTTTCCTGACAGACCCCGGTTGCATT
  CCTGTGACCCCTTCCC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000281 unedited
  GACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTCCAGTGATATAATAGT
  TTTATTTGAGACATAAAAACACATGTGTTTCTATTACATAGTGTGGGGTTTAGGGTCCTG
  GTTTCTAAGACAAGACTTTATTTCACCCTGTATCACAGCTTCCTGGGAAATGAATTAGGG
  AGCAAGAGACGGCCTGGCAAGAAAATCATTATTGTTGCTGGGAAGTTGCAAAGAAAGGGG
  AGAGTTTATTCAAATTAGTGTAACAGAGCCCCCAGGATGAAGAGAGTGGTGCAGGGAAAA
  GGTCTAAATTCCTGGTGTTGGTGGGGACACTGGCACATCCCACAGCAAGGACTCAGCCCT
  CAACGGCGGCGGCTGGGTCTTGGGAGGGGAGTGGTGGGAGGGTAAGGGCTCCTCAGCTCC
  CTCCCTGGACTCCCAGTTCAGTCACCCTTTCCCCCGGAAGAATTCAAAGAGGAAGGGCAG
  GGTCTATGTCATGGACACTGCTACTTGTTCGATGAAGCTGGCCAGGTTTATGTCCCGTTC
  TGAAAGGCCGGCACACTCATGGGTGCTCAGCGTGATGTGGACCTTGTTGTACACGTTAAA
  CCATTCAGGATGGTGGTCCAGTTTCTCAGCCTGCAGGGCCACTCTTGTCATGAACCCAAA
  GGCCCTGTTGAAGTCTTTGAAATGACACTGCTTGAAGATGGCATCACGGCCTTTCAGCTC
  ATTCCACCCACAGCCCTCAAGTTTGGCAGCAGCTGGTCCCCTCTCTCAGCGCTCAGCCTG
  TGTGCTTTGCCAGCATGGGCCCGGGCGGNCCTTGTTGCTAAATTNCGGCGCCGCCCTAAG
  GGATGCGGATTAAAAACTCCGCCGGTTCTTTAACGACCCCGCCTATATAAATTCACCACA
  GAACCCCTACGGCCATTGGGCAAAGGCGCGGGGCTTTCCCATTCCTGAAGTCCCT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000281
• Insert Size: 820 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000281.2, NP_000272.1
• RefSeq Size: 1025 bp
• RefSeq ORF: 315 bp
• Locus ID: 5092
• UniProt ID: P61457
• Cytogenetics: 10q22.1
• Domains: Pterin_4a
• Protein Families: Druggable Genome
• Gene Summary: This gene encodes a member of the pterin-4-alpha-carbinolamine dehydratase family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. The encoded protein functions as both a dehydratase involved in tetrahydrobiopterin biosynthesis, and as a cofactor for HNF1A-dependent transcription. A deficiency of this enzyme leads to hyperphenylalaninemia. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]
  Transcript Variant: This variant (1) encodes isoform 1.