ADPRH (NM_001125) Human Untagged Clone

CAT#: SC119411

ADPRH (untagged)-Human ADP-ribosylarginine hydrolase (ADPRH)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ADPRH
• Synonyms: ARH1; hARH1
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC119411 sequence for NM_001125 edited (data generated by NextGen Sequencing)
  ATGGAGAAGTATGTGGCTGCTATGGTGCTGAGTGCAGCTGGAGATGCCCTGGGGTACTAC
  AATGGGAAGTGGGAGTTCCTCCAGGATGGGGAGAAGATACACCGGCAGTTGGCCCAGCTG
  GGCGGCTTGGATGCCCTAGACGTGGGAAGGTGGAGAGTTAGTGACGACACAGTGATGCAC
  TTGGCCACAGCAGAAGCTCTTGTGGAAGCTGGGAAAGCCCCTAAGTTGACTCAACTGTAT
  TACCTCCTTGCTAAGCATTACCAAGACTGCATGGAAGACATGGATGGGCGGGCACCAGGT
  GGTGCCTCGGTGCACAACGCCATGCAGCTGAAGCCGGGCAAGCCCAATGGCTGGAGGATT
  CCCTTCAACAGCCATGAGGGCGGCTGTGGGGCTGCCATGCGGGCCATGTGCATCGGTCTC
  AGGTTCCCACACCATAGCCAACTGGACACACTGATCCAAGTGAGCATCGAGAGTGGTCGG
  ATGACCCACCACCACCCAACAGGCTACCTGGGGGCCCTTGCGTCTGCTCTTTTTACAGCC
  TATGCAGTGAATAGCAGACCACCCTTGCAGTGGGGAAAAGGACTGATGGAGCTGCTACCA
  GAAGCTAAAAAGTACATTGTCCAATCAGGCTACTTTGTAGAGGAAAATCTTCAACACTGG
  TCCTACTTCCAAACCAAATGGGAAAATTACCTAAAACTTAGAGGGATTTTGGATGGAGAA
  TCAGCCCCTACCTTCCCTGAGTCTTTCGGTGTGAAGGAGAGGGATCAGTTCTACACCTCC
  CTGAGCTACTCTGGCTGGGGTGGCAGCAGTGGGCACGATGCCCCCATGATTGCCTACGAT
  GCTGTTCTTGCTGCAGGAGACTCCTGGAAGGAGCTTGCCCACCGAGCCTTTTTCCATGGT
  GGAGACAGTGATTCTACAGCTGCCATTGCTGGCTGCTGGTGGGGAGTTATGTATGGTTTT
  AAAGGAGTGAGTCCCTCCAACTATGAGAAACTAGAATACAGAAACCGGCTGGAAGAGACA
  GCTAGGGCTTTATATTCTCTCGGGTCAAAAGAAGACACTGTAATTTCCCTTTAG
  
  Clone variation with respect to NM_001125.2
  546 t=>a
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001125 unedited
  GTAAGTACAAATTTTGNTAATACGACTCACTATAGGGCGGCCGCGATTCGGCACCAGGGC
  GACGGAGGTCCCGTCCACTCTCGCTTGGGTGCAGAAGGGCGCGGCGGTGCCAGGAAGCCG
  CCTTGGAGGCTCCAAGATTAGCTCACTCAGCGCGGCCAAGCGCAGGTGCGGGGGCCCTCG
  CCGCCACCCTGCTTATCTGGGGACAGTCCCGGAACCCGGTGACCCGGCTCCGCCCCAACC
  CGCGCCCTCGTCCACCCGCAGCAGCCAAGGCCTCTTCCCCGGCCCCGGGAGCCCGCGCCA
  CGCCCGTCGCTCGCGCGTTCCAGTCCAGGCTACAGCAGGGGCTCTCCAGCCTGCATTCCA
  TGGCCAACAGCAGTGCAGCTGCAGAAGCACTTCTGGAAGCCTCCTGACTTGAACTAGACC
  TTGGACACCCTCTCCAGAGCCCAGCAATTGTGAGGGACTGATGGAGAAGTATGTGGCTGC
  TATGGTGCTGAGTGCAGCTGGAGATGCCCTGGGGTACTACAATGGGAAGTGGGAGTTCCT
  CCAGGATGGGGAGAAGATACACCGGCAGTTGGCCCAGCTGGGCGGCTTGGATGCCCTAGA
  CGTGGGAAGGTGGAGAGTTAGTGACGACACAGTGATGCACTTGGCCACAGCAGAAGCTCT
  TGTGGAAGCTGGGAAAGCCCCTAAGTTGACTCAACTGTATTACCTCCTTGCTAAGCATTA
  CCAAGACTGCATGGAAGACATGGANTGGGCGGGCACCAGGTGGTGCCTCGGTGCACAACG
  CCATGCAGCTGAAGCCGGGCAAGCCCAATGGCTGGAGGATTCCCTNTCAACAGCCATGAG
  GCGGCTGTGGGGCTGCCATGCNGGCCATGTGCATCGGTCTCAGTTNNCCACCCATAGCCA
  ACTGGACCACTGATCCA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001125 unedited
  NNNGGGGTTTGCTATGGAACCGCGGCCGCAATCTAGNGATCGGTTTTTTTTTTTTTTTTT
  TTACGATGTGTCAGACCATCTTTATTGATTTACCCTAATGTTATGCATAAAGTTTATCTC
  TTCCCCCCATTCGTTCTCATCCTCCCTTCTCTCTCTCTCTCTCTCTTTCTCTGACACACA
  TACACACACATAAAGAAGCAAAGTCACCAGCTGAGAGCGAAGTTTGGGGTGGAAGTTTTG
  AGGTTTGAGAAAAAAGAAGTCTGAAAGTCTAACCTCTTTTTGAAATGTGCCTGTGATGAA
  GAGTGACAATCGGCAAAGCAAATCTAATAGGATCTCTAAAGAGCCTTTTCCTGTTGTTTA
  CAATTGTGGCTAAAGCCCTGCACTAGAAAACAACAAATGCTAAGATCCTCCCTGCATCCC
  CTACCCCCGACCCCCTGGGGCACACACAACCCTCCTCCACCACGTTCTAAAAAGACTGGA
  AGGACTTGGAAAAACATTCTTATGGGATGAACTAGAAGGTGGCAGGACTGCTATCATCAG
  ACCTCATTTACCCACCGCTTCCAGCTGAAAACCAGGGATTTCACAAAGGAAAAATCATGA
  GAGTCATAGTTCTGTGGTTTTCTCAAAAGGAGAAGGAAGGGACGCTCTTCCTTCCCACCC
  CTGAGAACAGGCCAAATGAGAGCAAGCCCCCTGGCTGGACTGAGGGTGACCAAATGAATG
  TGACGATGGCATCTCATTTCCCAGGTGGACATGTGCTGGGCAGCATGTGCCTAGGACTGA
  CCCAAAAACACCCAGCACCACACACCAGTATTGATGTGATGACCAGGCCCAAAACAAAAG
  GCTGTGGGAAGGAGTGAGGAGAAGGTGGGCCAATTGCCTGAACAAACAAAGCAGGT
  
• Restriction Sites: ECoRI-NOT
• ACCN: NM_001125
• Insert Size: 3510 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001125.2, NP_001116.1
• RefSeq Size: 3461 bp
• RefSeq ORF: 1074 bp
• Locus ID: 141
• UniProt ID: P54922
• Cytogenetics: 3q13.33
• Domains: ADP-ribosyl_GH
• Gene Summary: The enzyme encoded by this gene catalyzes removal of mono-ADP-ribose from arginine residues of proteins in the ADP-ribosylation cycle. Unlike the rat and mouse enzymes that require DTT for maximal activity, the human enzyme is DTT-independent. Alternatively spliced transcript variants that encode different protein isoforms have been described. [provided by RefSeq, May 2014]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longer protein. Variants 1 and 2 encode the same protein (isoform 1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.