Cyclin D1 (CCND1) (NM_053056) Human Untagged Clone

CAT#: SC119035

CCND1 (untagged)-Human cyclin D1 (CCND1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Cyclin D1
• Synonyms: BCL1; D11S287E; PRAD1; U21B31
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC119035 sequence for NM_053056 edited (data generated by NextGen Sequencing)
  ATGGAACACCAGCTCCTGTGCTGCGAAGTGGAAACCATCCGCCGCGCGTACCCCGATGCC
  AACCTCCTCAACGACCGGGTGCTGCGGGCCATGCTGAAGGCGGAGGAGACCTGCGCGCCC
  TCGGTGTCCTACTTCAAATGTGTGCAGAAGGAGGTCCTGCCGTCCATGCGGAAGATCGTC
  GCCACCTGGATGCTGGAGGTCTGCGAGGAACAGAAGTGCGAGGAGGAGGTCTTCCCGCTG
  GCCATGAACTACCTGGACCGCTTCCTGTCGCTGGAGCCCGTGAAAAAGAGCCGCCTGCAG
  CTGCTGGGGGCCACTTGCATGTTCGTGGCCTCTAAGATGAAGGAGACCATCCCCCTGACG
  GCCGAGAAGCTGTGCATCTACACCGACAACTCCATCCGGCCCGAGGAGCTGCTGCAAATG
  GAGCTGCTCCTGGTGAACAAGCTCAAGTGGAACCTGGCCGCAATGACCCCGCACGATTTC
  ATTGAACACTTCCTCTCCAAAATGCCAGAGGCGGAGGAGAACAAACAGATCATCCGCAAA
  CACGCGCAGACCTTCGTTGCCCTCTGTGCCACAGATGTGAAGTTCATTTCCAATCCGCCC
  TCCATGGTGGCAGCGGGGAGCGTGGTGGCCGCAGTGCAAGGCCTGAACCTGAGGAGCCCC
  AACAACTTCCTGTCCTACTACCGCCTCACACGCTTCCTCTCCAGAGTGATCAAGTGTGAC
  CCAGACTGCCTCCGGGCCTGCCAGGAGCAGATCGAAGCCCTGCTGGAGTCAAGCCTGCGC
  CAGGCCCAGCAGAACATGGACCCCAAGGCCGCCGAGGAGGAGGAAGAGGAGGAGGAGGAG
  GTGGACCTGGCTTGCACACCCACCGACGTGCGGGACGTGGACATCTGA
  
  Clone variation with respect to NM_053056.2
  723 g=>a
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_053056 unedited
  GTTCACATTTTGTATACGACTCACTATAGGCGGCCGCGAATCGGCACGAGGCGCAGTAGC
  AGCGAGCAGCAGAGTCCGCACGCTCCGGCGAGGGGCAGAAGAGCGCGAGGGAGCGCGGGG
  CAGCAGAAGCGAGAGCCGAGCGCGGACCCAGCCAGGACCCACAGCCCTCCCCAGCTGCCC
  AGGAAGAGCCCCAGCCATGGAACACCAGCTCCTGTGCTGCGAAGTGGAAACCATCCGCCG
  CGCGTACCCCGATGCCAACCTCCTCAACGACCGGGTGCTGCGGGCCATGCTGAAGGCGGA
  GGAGACCTGCGCGCCCTCGGTGTCCTACTTCAAATGTGTGCAGAAGGAGGTCCTGCCGTC
  CATGCGGAAGATCGTCGCCACCTGGATGCTGGAGGTCTGCGAGGAACAGAAGTGCGAGGA
  GGAGGTCTTCCCGCTGGCCATGAACTACCTGGACCGCTTCCTGTCGCTGGAGCCCGTGAA
  AAAGAGCCGCCTGCAGCTGCTGGGGGCCACTTGCATGTTCGTGGCCTCTAAGATGAAGGA
  GACCATCCCCCTGACGGCCGAGAAGCTGTGCATCTACACCGACAACTCCATCCGGCCCGA
  GGAGCTGCTGCAAATGGAGCTGCTCCTGGTGAACAAGCTCAAGTGGAACCTGCCCGCAAT
  GACCCCGCACGATTTCATTGAACACTTCCTCTCCAAAAGCCAGAAGCGGAGGAGAACANA
  CAGATCATCCGCAAACACGCGCAGACCCTTCGTGCCCTCTGTGCACAGATGTGAAGTTCA
  TTTCCATCCGCCCTCCATGTTGCANCGGCGAGCGTGGTGGCCGCACTGCAAGCCTGAACT
  TGAGAAGCCCAACAACTTCCTGTCTACTACGGCCTAAAACGTTTCTCTCCAGAGTGATCA
  AGGGGGACCCAAACTGCCTTC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_053056 unedited
  CCGCGGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTACATAGCCAAGATGTG
  CAAATTGTCTATTTTGTCACTAAGTTTTTTAAAGGAAGGGGCAGGGGATAAGAATTCATC
  GGAACCGAACTTAGGTTGAGTACCCTAATTTTCCTTGCACCCATGCCTGTCCAATCAGAT
  GACTCTGGGAAACGCCAAACAGGCTGAATCAATGTCTTTGTGTGGTTTTTTTCTTCCAGA
  TTGTTTTTTTCTCACCTATAAAAGGATCTATCTTTAAAAATAAACTGTATTAAATCTGTA
  ACATCAAAGGCAGAAGGTTTGTGTGTGTGTGTGTGTGTGTGTGTGTATCTGTGTGTTTAA
  ATCAAGGGGAGATTGCATTTATAAATCATACTGGCCTTATGAACATCCTCTGCAATAAAT
  ATACTTTTTAGCCTTAACTATAAATTATATATTTTAGTGTTTAAAAACCTTCCGGTGTGA
  AACATCTAAGATAACCCTTAAAAACCACCTGTTCTCTAGGTAAACCTCTGAGGTCCCTAC
  TTTCAAACACCAGTTGGCACCAAAGGATTCCTAAACTTCAACTTCTTTAAAGAAAAGGAA
  AGGAACTTATCATCCTGGCAATGTGAGAATGCAAACCTTTTTCTTCTTGACTGGCACGCA
  GCCTCCCAAACACCCCACCTTCACTGCCACACAGTGGCCCACACTTGCCTCAAAGTCTGC
  TTGCAAACCAGACATGGATATTCCAAACCTTTCCATTAAAAACTGCCCTCCTTGCACACC
  AACAAAAACAGCGTTATTTCCTCCACCTATTGTACTGAAATGCCTTGAAATGGAATGGTT
  TTATATTTGAACAAGAACCACAACCAGTTCCTGTGGGTTAACCCTGACACTAACCTGCTG
  CAGGGCCCTTGGTATAAAACCAACTTTTTTAAAGGTGTCTTCANAATTGGAAAGTTAAAA
  ATAT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_053056
• Insert Size: 2880 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_053056.1, NP_444284.1
• RefSeq Size: 4306 bp
• RefSeq ORF: 888 bp
• Locus ID: 595
• UniProt ID: P24385
• Cytogenetics: 11q13.3
• Domains: cyclin_C, CYCLIN, cyclin
• Protein Families: Druggable Genome, Stem cell - Pluripotency, Stem cell relevant signaling - DSL/Notch pathway, Stem cell relevant signaling - JAK/STAT signaling pathway, Stem cell relevant signaling - Wnt Signaling pathway
• Protein Pathways: Acute myeloid leukemia, Bladder cancer, Cell cycle, Chronic myeloid leukemia, Colorectal cancer, Endometrial cancer, Focal adhesion, Glioma, Jak-STAT signaling pathway, Melanoma, Non-small cell lung cancer, p53 signaling pathway, Pancreatic cancer, Pathways in cancer, Prostate cancer, Small cell lung cancer, Thyroid cancer, Viral myocarditis, Wnt signaling pathway
• Gene Summary: The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. This protein has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb. Mutations, amplification and overexpression of this gene, which alters cell cycle progression, are observed frequently in a variety of human cancers. [provided by RefSeq, Dec 2019]