HSD17B2 (NM_002153) Human Untagged Clone

CAT#: SC118783

HSD17B2 (untagged)-Human hydroxysteroid (17-beta) dehydrogenase 2 (HSD17B2)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: HSD17B2
• Synonyms: EDH17B2; HSD17; SDR9C2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_002153, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGAGCACTTTCTTCTCGGACACAGCATGGATCTGCCTGGCTGTCCCCACAGTACTATGTGGGACAGTAT
  TTTGCAAATACAAGAAGAGCTCAGGGCAGCTGTGGAGCTGGATGGTCTGCCTGGCAGGCCTCTGTGCAGT
  CTGCCTGCTCATCCTGTCCCCTTTTTGGGGCTTGATCCTCTTCTCGGTGTCATGCTTCCTCATGTATACT
  TACTTATCTGGCCAAGAATTGTTACCTGTGGATCAGAAGGCAGTCCTGGTGACAGGTGGTGATTGCGGGC
  TTGGCCATGCTTTGTGCAAGTATCTGGATGAGCTGGGCTTCACGGTATTTGCCGGAGTTTTGAATGAAAA
  TGGCCCAGGAGCTGAGGAATTGCGAAGAACCTGCTCTCCGCGCCTCTCGGTGCTCCAAATGGACATCACG
  AAGCCAGTGCAGATAAAAGATGCTTACAGCAAGGTTGCAGCAATGCTGCAGGACAGAGGACTGTGGGCTG
  TGATCAACAATGCTGGGGTGCTTGGCTTTCCAACTGATGGGGAGCTTCTTCTTATGACTGACTACAAACA
  ATGCATGGCCGTGAACTTCTTTGGAACTGTGGAGGTCACAAAGACGTTTTTGCCTCTTCTTAGAAAATCC
  AAAGGGAGGCTGGTGAATGTCAGCAGCATGGGAGGAGGGGCCCCAATGGAAAGGCTGGCATCTTATGGCT
  CATCAAAGGCGGCTGTGACCATGTTCTCATCAGTTATGAGACTGGAGCTTTCCAAGTGGGGAATTAAAGT
  TGCTTCCATCCAACCTGGAGGCTTCCTAACAAATATCGCAGGCACCAGTGACAAGTGGGAAAAGCTGGAG
  AAGGACATTCTGGACCACCTCCCCGCTGAGGTACAGGAAGACTACGGCCAGGACTACATCTTAGCACAGC
  GGAATTTCCTCCTATTGATCAACTCGTTAGCCAGCAAGGACTTCTCTCCGGTGCTGCGGGACATCCAGCA
  TGCTATCTTGGCGAAGAGCCCTTTTGCCTATTACACGCCAGGGAAAGGCGCTTACTTGTGGATCTGCCTT
  GCTCACTATTTGCCTATTGGCATATATGATTACTTTGCTAAAAGACATTTTGGCCAAGACAAGCCCATGC
  CCAGAGCTCTAAGAATGCCTAACTACAAGAAAAAGGCCACCTAG
  
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002153
• Insert Size: 1200 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002153.1, NP_002144.1
• RefSeq Size: 1427 bp
• RefSeq ORF: 1164 bp
• Locus ID: 3294
• UniProt ID: P37059
• Cytogenetics: 16q23.3
• Domains: adh_short
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Androgen and estrogen metabolism, Metabolic pathways
• Gene Summary: Capable of catalyzing the interconversion of testosterone and androstenedione, as well as estradiol and estrone. Also has 20-alpha-HSD activity. Uses NADH while EDH17B3 uses NADPH.[UniProtKB/Swiss-Prot Function]