NDUFS4 (NM_002495) Human Untagged Clone

CAT#: SC118622

NDUFS4 (untagged)-Human NADH dehydrogenase (ubiquinone) Fe-S protein 4, 18kDa (NADH-coenzyme Q reductase) (NDUFS4), nuclear gene encoding mitochondrial protein

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: NDUFS4
• Synonyms: AQDQ; CI-18; CI-18 kDa; CI-AQDQ; MC1DN1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC118622 sequence for NM_002495 edited (data generated by NextGen Sequencing)
  ATGGCGGCGGTCTCAATGTCAGTGGTACTGAGGCAGACGTTGTGGCGGAGAAGGGCAGTG
  GCTGTAGCTGCCCTTTCCGTTTCCAGGGTTCCGACCAGGTCGTTGAGGACTTCCACATGG
  AGATTGGCACAGGACCAGACTCAAGACACACAACTCATAACAGTTGATGAANNNNTGGAT
  ATCACTACTTTAACTGGCGTTCCAGAAGAGCATATAAAAACTAGAAAAGTCAGGATCTTT
  GTTCCTGCTCGCAATAACATGCAGTCTGGAGTAAACAACACAAAGAAATGGAAGATGGAG
  TTTGATACCAGGGAGCGATGGGAAAATCCTTTGATGGGTTGGGCATCAACGGCTGATCCC
  TTATCCAACATGGTTCTAACCTTCAGTACTAAAGAAGATGCAGTTTCCTTTGCAGAAAAA
  AATGGATGGAGCTATGACATTGAAGAGAGGAAGGTTCCAAAACCCAAGTCCAAGTCTTAT
  GGTGCAAACTTTTCTTGGAACAAAAGAACAAGAGTATCCACAAAATAG
  
  Clone variation with respect to NM_002495.2
  12 g=>c;172 a=>n;173 a=>n;174 a=>n;175 t=>n;198 a=>c;312 a=>g
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002495 unedited
  TCAAATTTTGTATACGACNACTATAGGGCGGCCGCGAATTCGCACGAGGAGCAAGATGGC
  GGCGGTCTCAATGTCAGTGGTACTGAGGCAGACGTTGTGGCGGAGAAGGGCAGTGGCTGT
  AGCTGCCCTTTCCGTTTCCAGGGTTCCGACCAGGTCGTTGAGGACTTCCACATGGAGATT
  GGCACAGGACCAGACTCAAGACACACAACTCATAACAGTTGATGAAAAATTGGTTTATCT
  TGTACCACTGTGTTTTACATTCTGTGGTCTAGCCACACCAAGCCTGGAACTTGCTACTCT
  TTTTCCTGCTTCAAGGCATTTGGAGTTCCTGTTCACTCTACCTGGATGCATTTTAACTAA
  TCCTGAGGACTTCATCCGGTCACCATCTACCTATTCTTCAAATGGATATCACTACTTTAA
  CTGGCGTTCCAGAAGAGCATATAAAAACTAGAAAAGTCAGGATCTTTGTTCCTGCTCGCA
  ATAACATGCAGTCTGGAGTAAACAACACAAAGAAATGGAAGATGGAGTTTGATACCAGGG
  AGCGATGGGAAAATCCTTTGATGGGTTGGGCATCAACGGCTGATCCCTTATCCAACATGG
  TTCTAACCTTCAGTACTAAAGAAGATGCAGTTTCCTTTGCAGAAAAAAATGGATGGAGCT
  ATGACATTGAAGAGAGGAAGGTTCCAAAACCCAAGTCCAAGTCTTATGGTGCAAACTTTT
  CTTGGAACAAAAGAACCAGAGTATCCACAAAATAGGTTGGCACTGACTATATCTCTGCTT
  GACTGTGAAATAAGTCAGCTATGCAGTATTTATAGTCCATGTATAATAAATACATCTCTT
  AATCTCCTAATANNATGGNACCTTTNAACTACANNAAAAAAAAAAAAAAACTCGACTCTA
  GATTGGGGNCCGCGTCATAGA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002495 unedited
  CGACGCTTCTAGAGTCGAGTTTTTTTTTTTTTTTTTTGTAGTTTAAAGGTCCAATTTATT
  AGGAGATTAAGAGATGTATTTATTATACATGGACTATAAATACTGCATAGCTGACTTTAT
  TCACAGTCAAGCAGAGATATAGTCAGTGCCAACCTATTTTGTGGATACTCTTGTTCTTTT
  GTTCCAAGAAAAGTTTGCACCATAAGACTTGGACTTGGGTTTTGGAACCTTCCTCTCTTC
  AATGTCATAGCTCCATCCATTTTTTTCTGCAAAGGAAACTGCATCTTCTTTAGTACTGAA
  GGTTAGAACCATGTTGGATAAGGGATCAGCCGTTGATGCCCAACCCATCAAAGGATTTTC
  CCATCGCTCCCTGGTATCAAACTCCATCTTACATTTCTTTGTGTTGCTTACTCCAGACTG
  CATGTTATTGCGAGCAGGAACAAAGATCCTGACTTTTCTAGTTTTTATATGCTCTTCTGG
  AACGCCAGCTAAAGTAGTGATATGCATTTGAAGAATAGGCACATGGCGACCGGATGAAGT
  CCTCAGGATTAGTTAAAATGCATCCAGGTACAGTGAACAGGAACTCCAAATGCCTTGAAG
  CACGAAAAAGAGTACCAAGTCCCAGGCTTGGTGTGGCTAGACCACAGAATGTAAAAACAG
  TGGTACAAGATAAACCAATTTTTCATCAACCTGTCTGAATTGCGTGTTTTGAGTCTGGTC
  CTGGGCCATCTTCATGGGGAAATTCTCACCACCTGTCCGGAACCCGGAAACGAAAGGCAG
  TACAGCCCTGCCCTTTTCGCCCAAAGTTGCCCTATCCCCGGACTTGAACCCCGCCTTTTG
  CTCTGTGCCGAATCCGGCCGCCTTATGAGTCCATACAAATTGACGTATTAAAAGCTTGTT
  TTTAAACCTCCACGAACCCTACGTCATTGTGTAGGGGGGGGTTACACATTGGAAAAG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002495
• Insert Size: 820 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002495.1, NP_002486.1
• RefSeq Size: 668 bp
• RefSeq ORF: 528 bp
• Locus ID: 4724
• UniProt ID: O43181
• Cytogenetics: 5q11.2
• Domains: ETC_CI_21
• Protein Families: Druggable Genome
• Protein Pathways: Alzheimer's disease, Huntington's disease, Metabolic pathways, Oxidative phosphorylation, Parkinson's disease
• Gene Summary: This gene encodes an nuclear-encoded accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (complex I, or NADH:ubiquinone oxidoreductase). Complex I removes electrons from NADH and passes them to the electron acceptor ubiquinone. Mutations in this gene can cause mitochondrial complex I deficiencies such as Leigh syndrome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2015]
  Transcript Variant: This variant (1) encodes the longer isoform (1).