TTF1 (NKX2-1) (NM_003317) Human Untagged Clone

CAT#: SC118030

NKX2 (untagged)-Human NK2 homeobox 1 (NKX2-1), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TTF1
• Synonyms: BCH; BHC; NK-2; NKX2.1; NKX2A; NMTC1; T/EBP; TEBP; TITF1; TTF-1; TTF1
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC118030 sequence for NM_003317 edited (data generated by NextGen Sequencing)
  ATGTCGATGAGTCCAAAGCACACGACTCCGTTCTCAGTGTCTGACATCTTGAGTCCCCTG
  GAGGAAAGCTACAAGAAAGTGGGCATGGAGGGCGGCGGCCTCGGGGCTCCGCTGGCGGCG
  TACAGGCAGGGCCAGGCGGCACCGCCAACAGCGGCCATGCAGCAGCACGCCGTGGGGCAC
  CACGGCGCCGTCACCGCCGCCTACCACATGACGGCGGCGGGGGTGCCCCAGCTCTCGCAC
  TCCGCCGTGGGGGGCTACTGCAACGGCAACCTGGGCAACATGAGCGAGCTGCCGCCGTAC
  CAGGACACCATGAGGAACAGCGCCTCTGGCCCCGGATGGTACGGCGCCAACCCAGACCCG
  CGCTTCCCCGCCATCTCCCGCTTCATGGGCCCGGCGAGCGGCATGAACATGAGCGGCATG
  GGCGGCCTGGGCTCGCTGGGGGACGTGAGCAAGAACATGGCCCCGCTGCCAAGCGCGCCG
  CGCAGGAAGCGCCGGGTGCTCTTCTCGCAGGCGCAGGTGTACGAGCTGGAGCGACGCTTC
  AAGCAACAGAAGTACCTGTCGGCGCCGGAGCGCGAGCACCTGGCCAGCATGATCCACCTG
  ACGCCCACGCAGGTCAAGATCTGGTTCCAGAACCACCGCTACAAAATGAAGCGCCAGGCC
  AAGGACAAGGCGGCGCAGCAGCAACTGCAGCAGGACAGCGGCGGCGGCGGGGGCGGCGGG
  GGCACCGGGTGCCCGCAGCAGCAACAGGCTCAGCAGCAGTCGCCGCGACGCGTGGCGGTG
  CCGGTCCTGGTGAAAGACGGCAAACCGTGCCAGGCGGGTGCCCCCGCGCCGGGCGCCGCC
  AGCCTACAAGGCCACGCGCAGCAGCAGGCGCAGCACCAGGCGCAGGCCGCGCAGGCGGCG
  GCAGCGGCCATCTCCGTGGGCAGCGGTGGCGCCGGCCTTGGCGCACACCCGGGCCACCAG
  CCAGGCAGCGCAGGCCAGTCTCCGGACCTGGCGCACCACGCCGCCAGCCCCGCGGCGCTG
  CAGGGCCAGGTATCCAGCCTGTCCCACCTGAACTCCTCGGGCTCGGACTACGGCACCATG
  TCCTGCTCCACCTTGCTATACGGTCGGACCTGGTGA
  
  Clone variation with respect to NM_003317.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003317 unedited
  NGTCGCGATTAGTATACGACTCCTATAGGGCGGCCGCGAATTCGCACGAGGCTGACAGAC
  ACGTAGACCAACAGTGCGGCCCCAGGTTCGTCCCCAGACTCGCTCGCTCATTTGTTGGCG
  ACTGGGGCTCAGCGCAGCGAAGCCCGATGTGGTCCGGAGGCAGTGGGAAGGCGCGGGGCT
  GGGAGGCCGCGGCGGGAGGGAGGAGCAGCCCCGGCAGGCTCAGCCGCCGCCGAATCATGT
  CGATGAGTCCAAAGCACACGACTCCGTTCTCAGTGTCTGACATCTTGAGTCCCCTGGAGG
  AAAGCTACAAGAAAGTGGGCATGGAGGGCGGCGGCCTCGGGGCTCCGCTGGCGGCGTACA
  GGCAGGGCCAGGCGGCACCGCCAACAGCGGCCATGCAGCAGCACGCCGTGGGGCACCACG
  GCGCCGTCACCGCCGCCTACCACATGACGGCGGCGGGGGTGCCCCAGCTCTCGCACTCCG
  CCGTGGGGGGCTACTGCAACGGCAACCTGGGCAACATGAGCGAGCTGCCGCCGTACCAGG
  ACACCATGAGGAACAGCGCCTCTGGCCCCGGATGGTACGGCGCCAACCCAGACCCGCGCT
  TCCCCGCCATCTCCCGCTTCATGGGCCCGGCGAGCGGCATGAACATGAGCGGCATGGGCG
  GCCTGNGCTCGCTGGGGGACGTGAGCAAGAACATGGCCCCGCTGCCAAGCGCGCCGCGCA
  GGAAGCGCCGGGTGCTCTTCTCGCAGGCGCAGGTGTACGAGCTGGAGCGACGCTTCAAGC
  AACAGAAGTACCCTGTCGCGCCGGAGCGCGAGCACCTGGCCAGCATGATCCACCTGACGC
  CCACGCAGTCAGATCTGGTTCCAGAACCACCGCTACANATGAAGCGCCAGNNCAGGNACA
  NGGCNGCGCAGCAGCAACTGCAGCAGGACAGC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_003317 unedited
  GCGTTAAGGAGACTTTATTATTTTTTTAATTTATTAGNAGCTTCTTAGGGACTATATTTA
  AGCCAAATATCTACATAAGTTACAACAGAAAAAGATGACGCCGCAAATACCAAACTGCCA
  AATAATATACACAGATTTGTCAATGCCCATAAAAAATGTGAAGGGCTGGGGACTGGGAGT
  GGTTTTTCTTTTTACAACAAAATGTACAGATTACTAAAAACTAGGCATTTAGTCCAACTT
  TTGACAGCGTTTTACAGCTACAAGTTCACATTAAACAAACTATTTTCGCGGAGGGCGGTC
  GCCGCTGAGCCTAGGCGGCCAGAGGGTGCCGGGGAGGGGGCACTTCCTTTGTGTCAGTGA
  CAAGTGGGTTATGTTGAAGACTCTTTCCTCTCCCCAGCTCCCGTCCTCCCTTCAAAAAAA
  AAAAAAAAATCCTGGTATTTACAAGCGAGTCCTCTTTGCTGGCAGAGTGTGCCCAGAGTG
  AAGTTTGGTCTTTAGAGTCCAGAGCCATGTCAGCACAGAGCCCTCTCCAATCTGTGCCCC
  GCCCCTAGCGTGGAAAACCCATTTGAATCACCAAAAAAAGACACCCCAAAGCTGTTTTAT
  GCCCTTTTCTGCTTAAAGATTCCTTGAGATTGGATGCGCTTGGCTGCTCTTCATTTTCTT
  TTTTTTAAAAAAAAAAAACCCCCAAATTTTCAGGGGGGGAAAAAAACAAAGAAGTCCCAC
  CACTTAGGCCTTTGTGGTTTTTTTGTTCCTTGGGCCTAAAACCGGCCCGNTTCTAAAGAA
  AATTTAACGCGTTGGAACAACGGTGGATGGTGCTTCTTTGGCTGCCAGAAGGAACCGTGA
  AGCCAACCCTGGGCTTAGGCCGACCGGTGTCTTTTACCAATTCCAACCGTTTCAAGGTGT
  ACCGAATTGGGCCCCTTTCCACACCAG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_003317
• Insert Size: 2300 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003317.3, NP_003308.1
• RefSeq Size: 2352 bp
• RefSeq ORF: 1116 bp
• Locus ID: 7080
• UniProt ID: P43699
• Cytogenetics: 14q13.3
• Domains: homeobox
• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Transcription Factors
• Gene Summary: This gene encodes a protein initially identified as a thyroid-specific transcription factor. The encoded protein binds to the thyroglobulin promoter and regulates the expression of thyroid-specific genes but has also been shown to regulate the expression of genes involved in morphogenesis. Mutations and deletions in this gene are associated with benign hereditary chorea, choreoathetosis, congenital hypothyroidism, and neonatal respiratory distress, and may be associated with thyroid cancer. Multiple transcript variants encoding different isoforms have been found for this gene. This gene shares the symbol/alias 'TTF1' with another gene, transcription termination factor 1, which plays a role in ribosomal gene transcription. [provided by RefSeq, Feb 2014]
  Transcript Variant: This variant (2) differs in the 5' UTR and coding region compared to variant 1. The resulting protein (isoform 2) is shorter and has a distinct N-terminus compared to isoform 1.