H4C14 (NM_003548) Human Untagged Clone

CAT#: SC117933

HIST2H4A (untagged)-Human histone cluster 2, H4a (HIST2H4A)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: H4C14
• Synonyms: FO108; H4; H4-16; H4/n; H4C1; H4C2; H4C3; H4C4; H4C5; H4C6; H4C8; H4C9; H4C11; H4C12; H4C13; H4C15; H4F2; H4FN; HIST2H4; HIST2H4A
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_003548 edited
  ATGTCCGGCAGAGGAAAGGGCGGAAAAGGCTTAGGCAAAGGGGGCGCTAAGCGCCACCGC
  AAGGTCTTGAGAGACAACATTCAGGGCATCACCAAGCCTGCCATTCGGCGTCTAGCTCGG
  CGTGGCGGCGTTAAGCGGATCTCTGGCCTCATTTACGAGGAGACCCGCGGTGTGCTGAAG
  GTGTTCCTGGAGAATGTGATTCGGGACGCAGTCACCTACACCGAGCACGCCAAGCGCAAG
  ACCGTCACAGCCATGGATGTGGTGTACGCGCTCAAGCGCCAGGGGCGCACCCTGTACGGC
  TTCGGAGGCTAG
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003548 unedited
  CACTATAGGTGCCGGCCGCGTAATTCGGCACGAGGCTCCAGCGGTCATGTCCGGCAGAGG
  AAAGGGCGGAAAAGGCTTAGGCAAAGGGGGCGCTAAGCGCCACCGCAAGGTCTTGAGAGA
  CAACATTCAGGGCATCACCAAGCCTGCCATTCGGCGTCTAGCTCGGCGTGGCGGCGTTAA
  GCGGATCTCTGGCCTCATTTACGAGGAGACCCGCGGTGTGCTGAAGGTGTTCCTGGAGAA
  TGTGATTCGGGACGCAGTCACCTACACCGAGCACGCCAAGCGCAAGACCGTCACAGCCAT
  GGATGTGGTGTACGCGCTCAAGCGCCAGGGGCGCACCCTGTACGGCTTCGGAGGCTAGGC
  CGCCGCTCCAGCTTTGCGCGTTTCGATCCCAAAGGCCCTTTTTAGGGCCGACCACTTGCT
  CATCTGAGGAGTTGGACACTTGACTGCGTAAAGTGCAACAGTAACGATGTTGGAAGGTAA
  CTTTGGCAGTGGGGCGACAATCGGATCTGAAGTTACGGANAGCTACCGCGGCCCATAGCG
  CTCACAGCCGTAAAGACTTAAGTCGTTGACCGAAAGCGGCTTTTTCACTTACCTGGGCTT
  TTTTTTTTTTTTTTTTTTTTAAAGCCTTTATCGGTATGAAAGGTTGAATGCTCTANGTTT
  CAGCACTGCTTTCTCGGCTTGCTCTTCTGGTGCAGTATAGGCACACCTAGAGGGCCACGT
  CAGTCTTTGCGATCACCAAATCTGGTTCTGAGAAATANGCACTGGCAATTTACACATGCC
  TTTGCTGTGTAATCTCACTATATTTTGCTCAGGCAAGTGGGGAGAAGCAGCCTTNAGGTT
  TTCATTCTAGAGATGCCGGCTTTCCCACCTGATCGGCTTTAGAGTCC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_003548
• Insert Size: 2740 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003548.2, NP_003539.1
• RefSeq Size: 396 bp
• RefSeq ORF: 312 bp
• Locus ID: 8370
• UniProt ID: P62805
• Cytogenetics: 1q21.2
• Domains: H4, histone
• Protein Pathways: Systemic lupus erythematosus
• Gene Summary: Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H4 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in a histone cluster on chromosome 1. This gene is one of four histone genes in the cluster that are duplicated; this record represents the centromeric copy. [provided by RefSeq, Aug 2015]