CILP (NM_003613) Human Untagged Clone

CAT#: SC117885

CILP (untagged)-Human cartilage intermediate layer protein, nucleotide pyrophosphohydrolase (CILP)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CILP
• Synonyms: CILP-1; HsT18872
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC117885 sequence for NM_003613 edited (data generated by NextGen Sequencing)
  ATGGTGGGGACCAAGGCCTGGGTGTTCTCCTTCCTGGTCCTGGAAGTCACATCTGTGTTG
  GGGAGACAGACGATGCTCACCCAGTCAGTAAGAAGAGTCCAGCCTGGGAAGAAGAACCCC
  AGCATCTTTGCCAAGCCTGCCGACACCCTGGAGAGCCCTGGTGAGTGGACAACATGGTTC
  AACATCGACTACCCAGGCGGGAAGGGCGACTATGAGCGGCTGGACGCCATTCGCTTCTAC
  TATGGGGACCGTGTATGTGCCCGTCCCCTGCGGCTAGAGGCTCGGACCACTGACTGGACA
  CCTGCGGGCAGCACTGGCCAGGTGGTCCATGGTAGTCCCCGTGAGGGTTTCTGGTGCCTC
  AACAGGGAGCAGCGGCCTGGCCAGAACTGCTCTAATTACACCGTACGCTTCCTCTGCCCA
  CCAGGATCCCTGCGCCGAGACACAGAGCGCATCTGGAGCCCATGGTCTCCCTGGAGCAAG
  TGCTCAGCTGCCTGTGGTCAGACTGGGGTCCAGACTCGCACACGCATTTGCTTGGCAGAG
  ATGGTGTCGCTGTGCAGTGAGGCCAGCGAAGAGGGTCAGCACTGCATGGGCCAGGACTGT
  ACAGCCTGTGACCTGACCTGCCCAATGGGCCAGGTGAATGCTGACTGTGATGCCTGCATG
  TGCCAGGACTTCATGCTTCATGGGGCTGTCTCCCTTCCCGGAGGTGCCCCAGCCTCAGGG
  GCTGCTATCTACCTCCTGACCAAGACGCCGAAGCTGCTGACCCAGACAGACAGTGATGGG
  AGATTCCGAATCCCTGGCTTGTGCCCTGATGGCAAAAGCATCCTGAAGATCACAAAGGTC
  AAGTTTGCCCCCATTGTACTCACAATGCCCAAGACTAGCCTGAAGGCAGCCACCATCAAG
  GCAGAGTTTGTGAGGGCAGAGACTCCATACATGGTGATGAACCCTGAGACAAAAGCACGG
  AGAGCTGGGCAGAGCGTGTCTCTGTGCTGTAAGGCCACAGGGAAGCCCAGGCCAGACAAG
  TATTTTTGGTATCATAATGACACATTGCTGGATCCTTCCCTCTACAAGCATGAGAGCAAG
  CTGGTGCTGAGGAAACTGCAGCAGCACCAGGCTGGGGAGTACTTTTGCAAGGCCCAGAGT
  GATGCTGGGGCTGTGAAGTCCAAGGTTGCCCAGCTGATTGTCATAGCATCTGATGAGACT
  CCTTGCAACCCAGTTCCTGAGAGCTATCTTATCCGGCTGCCCCATGATTGCTTTCAGAAT
  GCCACCAACTCCTTCTACTATGACGTGGGACGCTGCCCTGTTAAGACTTGTGCAGGGCAG
  CAGGATAATGGGATCAGGTGCCGTGATGCTGTGCAGAACTGCTGTGGCATCTCCAAGACA
  GAGGAAAGGGAGATCCAGTGCAGTGGCTACACGCTACCCACCAAGGTGGCCAAGGAGTGC
  AGCTGCCAGCGGTGTACGGAAACTCGGAGCATCGTGCGGGGCCGTGTCAGTGCTGCTGAC
  AATGGGGAGCCCATGCGCTTTGGCCATGTGTACATGGGGAACAGCCGTGTAAGCATGACT
  GGCTACAAGGGCACTTTCACCCTCCATGTCCCCCAGGACACTGAGAGGCTGGTGCTCACA
  TTTGTGGACAGGCTGCAGAAGTTTGTCAACACCACCAAAGTGCTACCTTTCAACAAGAAG
  GGGAGTGCCGTGTTCCATGAAATCAAGATGCTTCGTCGGAAAGAGCCCATCACTTTGGAA
  GCCATGGAGACCAACATCATCCCCCTGGGGGAAGTGGTTGGTGAAGACCCCATGGCTGAA
  CTGGAGATTCCATCCAGGAGTTTCTACAGGCAGAATGGGGAGCCCTACATAGGAAAAGTG
  AAGGCCAGTGTGACCTTCCTGGATCCCCGGAATATTTCCACAGCCACAGCTGCCCAGACT
  GACCTGAACTTCATCAATGACGAAGGAGACACTTTCCCCCTTCGGACGTATGGCATGTTC
  TCTGTGGACTTCAGAGATGAGGTCACCTCAGAGCCACTTAATGCTGGCAAAGTGAAGGTC
  CACCTTGACTCGACCCAGGTCAAGATGCCAGAGCACATATCCACAGTGAAACTCTGGTCA
  CTCAATCCAGACACAGGGCTGTGGGAGGAGGAAGGTGATTTCAAATTTGAAAATCAAAGG
  AGGAACAAAAGAGAAGACAGAACCTTCCTGGTGGGCAACCTGGAGATTCGTGAGAGGAGG
  CTCTTTAACCTGGATGTTCCTGAAAGCAGGCGGTGCTTTGTTAAGGTGAGGGCCTACCGG
  AGTGAGAGGTTCTTGCCTAGTGAGCAGATCCAGGGGGTTGTGATCTCCGTGATTAACCTG
  GAGCCTAGAACTGGCTTCTTGTCCAACCCTAGGGCCTGGGGCCGCTTTGACAGTGTCATC
  ACAGGCCCCAACGGGGCCTGTGTGCCTGCCTTCTGTGATGACCAGTCCCCTGATGCCTAC
  TCTGCCTATGTCTTGGCAAGCCTGGCTGGGGAGGAACTGCAAGCAGTGGAGTCTTCTCCT
  AAATTCAACCCAAATGCAATTGGCGTCCCTCAGCCCTATCTCAACAAGCTCAACTACCGT
  CGGACGGACCATGAGGATCCACGGGTTAAAAAGACAGCTTTCCAGATTAGCATGGCCAAG
  CCAAGGCCCAACTCAGCTGAGGAGAGCAATGGGCCCATCTATGCCTTTGAGAACCTCCGG
  GCATGTGAAGAGGCACCACCCAGTGCAGCCCACTTCCGGTTCTACCAGATTGAGGGGGAT
  CGATATGACTACAACACAGTCCCCTTCAACGAAGATGACCCTATGAGCTGGACTGAAGAC
  TATCTGGCATGGTGGCCAAAGCCGATGGAATTCAGGGCCTGCTATATCAAGGTGAAGATT
  GTGGGGCCACTGGAAGTGAATGTGCGATCCCGCAACATGGGGGGCACTCATCGGCGGACA
  GTGGGGAAGCTGTATGGAATCCGAGATGTGAGGAGCACTCGGGACAGGGACCAGCCCAAT
  GTCTCAGCTGCCTGTCTGGAGTTCAAGTGCAGTGGGATGCTCTATGATCAGGACCGTGTG
  GACCGCACCCTGGTGAAGGTCATCCCCCAGGGCAGCTGCCGTCGAGCCAGTGTGAACCCC
  ATGCTGCATGAGTACCTGGTCAACCACTTGCCACTTGCAGTCAACAACGACACCAGTGAG
  TACACCATGCTGGCACCCTTGGACCCACTGGGCCACAACTATGGCATCTACACTGTCACT
  GACCAGGACCCTCGCACGGCCAAGGAGATCGCGCTCGGCCGGTGCTTTGATGGCACATCC
  GATGGCTCCTCCAGAATCATGAAGAGCAATGTGGGAGTAGCCCTCACCTTCAACTGTGTA
  GAGAGGCAAGTAGGCCGCCAGAGTGCCTTCCAGTACCTCCAAAGCACCCCAGCCCAGTCC
  CCTGCTGCAGGCACTGTCCAAGGAAGAGTGCCCTCGAGGAGGCAGCAGCGAGCGAGCAGG
  GGTGGCCAGCGCCAGAGTGGAGTGGTGGCCTCTCTGAGATTTCCTAGAGTTGCTCAACAG
  CCCCTGATCAACTAA
  
  Clone variation with respect to NM_003613.3
  2936 a=>g;3496 g=>a
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003613 unedited
  GGTCAGGATTTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGCAGCTGAGG
  AGTCCTGCTCAAAACACGGTCACTGGATCTGAGAACTTCCCAGGGGACCGCATTCCAGAG
  TCAGTGACTCTGTGAAGCACCCACATCTACCTCTTGCCACGTTCCCACGGGCTTGGGGGA
  AAGATGGTGGGGACCAAGGCCTGGGTGTTCTCCTTCCTGGTCCTGGAAGTCACATCTGTG
  TTGGGGAGACAGACGATGCTCACCCAGTCAGTAAGAAGAGTCCAGCCTGGGAAGAAGAAC
  CCCAGCATCTTTGCCAAGCCTGCCGACACCCTGGAGAGCCCTGGTGAGTGGACAACATGG
  TTCAACATCGACTACCCAGGCGGGAAGGGCGACTATGAGCGGCTGGACGCCATTCGCTTC
  TACTATGGGGACCGTGTATGTGCCCGTCCCCTGCGGCTAGAGGCTCGGACCACTGACTGG
  ACACCTGCGGGCAGCACTGGCCAGGTGGTCCATGGTAGTCCCCGTGAGGGTTTCTGGTGC
  CTCAACAGGGAGCAGCGGCCTGGCCAGAACTGCTCTAATTACACCGTACGCTTCCTCTGC
  CCACCAGGATCCCTGCGCCGAGACACAGAGCGCATCTGGAGCCCATGGTCTCCCTGGAGC
  AAGTGCTCAGCTGCCTGTGGTCAGACTGGGGTCCAGACTCGCACACGCATTTGCTTGGCA
  GAGATGGTGTCGCTGTGCAGTGAGGCCAGCGAAGAGGGTCAGCACTGCATGGGCCAGGAC
  TGTACAGCCTGTGACCTGACCTGCCCAATGGGCCANGTGAATGCTGACTGTGATGCCTGC
  ATGTGCCANGACTTCATGCTTCATGGGGCTGTCTCCTTTNCCGNAGTGCCCCAGCTCAGG
  GGCTGCTATCTACTCCTGACN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_003613 unedited
  GCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTACAGAGTATGCATTT
  ATTGACATCTGCCCTGGGCTACACTGGGTTTCTAGCAAGAACAAAAGCGAATGCAAAGGG
  AAATGTACAAATACAGTTAGCAACCTGGTGTAATTGGGGATACCAGGAGATGTTCTTTCC
  AGACTTGGTAGAGGAACTATGATCACCCTATATTTAATAGCTTAACTTTGAATGTGCACT
  AAAATTCCAAATTTAGCAAAGAAATAAATAAGTTATTTACATTTCAAGTAAAAGTATTGG
  GTTTTATTTCTTTATTTCACCACCATTTATATGTATGAACTATGATCAAGGCTTGCCTAT
  GTCCTGTGGAGCAAGGCCAAGAAATATGCTTTATATTGGTTATTTGATCTTCAGCATCAG
  ATTATCACTATTGCAGAGGTGGGCAAAACCATGCAAAAAGAAGAGGAAGTATATTTGCAT
  TAATGGCATTAAATGAAGTACAGTTGAAGCTGCAGAGTTTTACCAGTGGCCAATTTCTTG
  TGTTTCATTAAAAGAACATGTTCACAAAGGGGCCTTATTGTGCCATTGTGGGGGGCACGT
  GCCATTCTTTGCCTGGGACAAAGTAAGTAAAGGCTCGAACAACCATCAGTCATATCCCGA
  AAACAAAATGCCTAAATAAACCAAGGGACAGTTGGCCTTACTTTCACAAGGGTTGTTATG
  GAATGAGGGGCGACAAGGCGCATTACCCCACACCTATTGCTCACGCGCGGCTGGTCACTT
  TAGGAATTCTATAAGGGCACCTTNCCTCTGGCCCGTGCACCCCCGCTCCTACACTCCGCT
  TTCAACACGGCTTCCCCCTCATCTCTCTCCTAAACGGCCGCCGCGAGCGCCTTCCTGTTC
  TTAACGACTCTCTCGCCCACTCCTCTCCCCTTTTGGACTAGGCCTCCTCTCACTCTCTCC
  ATTTCCTGCCTACCCGTG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_003613
• Insert Size: 4760 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003613.2, NP_003604.2
• RefSeq Size: 4436 bp
• RefSeq ORF: 3555 bp
• Locus ID: 8483
• UniProt ID: O75339
• Cytogenetics: 15q22.31
• Domains: tsp_1, ig, IGc2, IG
• Protein Families: Druggable Genome, Phosphatase, Secreted Protein
• Gene Summary: Major alterations in the composition of the cartilage extracellular matrix occur in joint disease, such as osteoarthrosis. This gene encodes the cartilage intermediate layer protein (CILP), which increases in early osteoarthrosis cartilage. The encoded protein was thought to encode a protein precursor for two different proteins; an N-terminal CILP and a C-terminal homolog of NTPPHase, however, later studies identified no nucleotide pyrophosphatase phosphodiesterase (NPP) activity. The full-length and the N-terminal domain of this protein was shown to function as an IGF-1 antagonist. An allelic variant of this gene has been associated with lumbar disc disease. [provided by RefSeq, Sep 2010]