HSD17B6 (NM_003725) Human Untagged Clone

CAT#: SC117792

HSD17B6 (untagged)-Human hydroxysteroid (17-beta) dehydrogenase 6 homolog (mouse) (HSD17B6)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: HSD17B6
• Synonyms: HSE; RODH; SDR9C6
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_003725, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGTGGCTCTACCTGGCGGCCTTCGTGGGCCTGTACTACCTTCTGCACTGGTACCGGGAGAGGCAGGTGG
  TGAGCCACCTCCAAGACAAGTATGTCTTTATCACGGGCTGTGACTCGGGCTTTGGGAACCTGCTGGCCAG
  ACAGCTGGATGCACGAGGCTTGAGAGTGCTGGCTGCGTGTCTGACGGAGAAGGGGGCCGAGCAGCTGAGG
  GGCCAGACGTCTGACAGGCTGGAGACGGTGACCCTGGATGTTACCAAGATGGAGAGCATCGCTGCAGCTA
  CTCAGTGGGTGAAGGAGCATGTGGGGGACAGAGGACTCTGGGGACTGGTGAACAATGCAGGCATTCTTAC
  ACCAATTACCTTATGTGAGTGGCTGAACACTGAGGACTCTATGAATATGCTCAAAGTGAACCTCATTGGT
  GTGATCCAGGTGACCTTGAGCATGCTTCCTTTGGTGAGGAGAGCACGGGGAAGAATTGTCAATGTCTCCA
  GCATTCTGGGAAGAGTTGCTTTCTTTGTAGGAGGCTACTGTGTCTCCAAGTATGGAGTGGAAGCCTTTTC
  AGATATTCTGAGGCGTGAGATTCAACATTTTGGGGTGAAAATCAGCATAGTTGAACCTGGCTACTTCAGA
  ACGGGAATGACAAACATGACACAGTCCTTAGAGCGAATGAAGCAAAGTTGGAAAGAAGCCCCCAAGCATA
  TTAAGGAGACCTATGGACAGCAGTATTTTGATGCCCTTTACAATATCATGAAGGAAGGGCTGTTGAATTG
  TAGCACAAACCTGAACCTGGTCACTGACTGCATGGAACATGCTCTGACATCGGTGCATCCGCGAACTCGA
  TATTCAGCTGGCTGGGATGCTAAATTTTTCTTCATCCCTCTATCTTATTTACCTACATCACTGGCAGACT
  ACATTTTGACTAGATCTTGGCCCAAACCAGCCCAGGCAGTCTAA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003725 unedited
  GGTCAAAAATTGTATACGACTCATATAGGCGGCCGCGNAATCGGCACGAGGAGGAGGAAG
  CCGACTGCTGCCTGGTCTGCAAAGAAGTCCTTTCAAGTCTCTAGGACTGGACTCTTCCTA
  AGCAAGTCCGAGAAGGAAGCACCCTCACTATGTGGCTCTACCTGGCGGCCTTCGTGGGCC
  TGTACTACCTTCTGCACTGGTACCGGGAGAGGCAGGTGGTGAGCCACCTCCAAGACAAGT
  ATGTCTTTATCACGGGCTGTGACTCGGGCTTTGGGAACCTGCTGGCCAGACAGCTGGATG
  CACGAGGCTTGAGAGTGCTGGCTGCGTGTCTGACGGAGAAGGGGGCCGAGCAGCTGAGGG
  GCCAGACGTCTGACAGGCTGGAGACGGTGACCCTGGATGTTACCAAGATGGAGAGCATCG
  CTGCAGCTACTCAGTGGGTGAAGGAGCATGTGGGGGACAGAGGACTCTGGGGACTGGTGA
  ACAATGCAGGCATTCTTACACCAATTACCTTATGTGAGTGGCTGAACACTGAGGACTCTA
  TGAATATGCTCAAAGTGAACCTCATTGGTGTGATCCAGGTGACCTTGAGCATGCTTCCTT
  TGGTGAGGAGAGCACGGNGAAGAATTGTCAATGTCTCCAGCATTCTGGGAAGAGTTGCTT
  TCTTTGTAGGAGGCTACTGTGTCTCCAAGTATGGAGTGGAAGCCTTTTCAGATATTCTGA
  GGCGTGAGATTCAACATTNTGGGGTGAAAATCAGCATAGTTGAACCTGGCTACTTCAGAA
  CGGGAATGACAAACATGACACAGTCCTTAGAGCGAATGAAGCANAGTTGGAAAGAAGCCC
  CCAGCATATTAAGGAGACCTATGGACAGCAGTATTTTGGATGCCCCTTACATATCATGAA
  GGAAAGGCT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_003725 unedited
  AAATACAATATTCTATTATAAAGTACCATAATACANACCCATTTCAAGTAAGGACAAACA
  CACCAACATATTTCTTAGTAGTTTCCTCACAATAGATTATTAAAGCATAGAACAATTATT
  CATATTCATAAAGAAATGACTTCAAAATAGGTTAAATTGTTTTCCATCTACTCTGTTTAA
  TAAGGCAAGAACAAATGATTCACTTTAGACAAATAGTCTCATCAAAAAAGGGCTAAAATA
  GTAAAGATTCATCACCTAAAGTGGTAAGCTTTGGATATCTGAAATATAAACATGTTAGTA
  CTCTGATGATGCCAGATAAATGAATTCAGGCAAGAAAACACATCGTTACAAAAAGCCTGG
  GTTCTAAATCAGGATTACTGAGACACTAACAATTTCAGATTTTTGTCTTCATTCCAAGAA
  GCACCAACCCAGCTTTCTTTAGACTGCCTGGGCTGGTCTGGGCCACCATCTAGTCAAAAT
  GTCGTCTGCCACCGACGTAGGTCAATAAGATACACGGATCAATCAAAATGTACCCTCCCA
  CCCACCTGAATATCTACCTCTTGGATGCCCCGCCGCCACAGCACGTTCCATGCAGTCATC
  TGACCACCTATATGGTTTGTGCTGCAATCCCCCCCCCCCCGCCTTCATGATCTTTTCCGA
  GTCCTCGCCCTTCTCGTTGTCATAGGCCTCCTTCACTCACCCTCCGGCCGTCTCTCACCC
  TCCCCCCCACCCTTATCACGCTCCCCCCCCCCGCCCTTCTCCTGTGAATTCCCTTTCCAC
  CCCCCTCCTGTCACCCGAAAGTTCCTCCACCCTCCCAATCGCCCAATCTTCCCTCCCCCT
  TACATCCTTCCTCCCCACCCCCGTTTTTTTCCCCCCCGAAACATTGCTTCTTCCTCCCTC
  CCCCCATTCTAGCCTCCCACGTCTCCCCTCCCCATCATTTTCTTCCCCTCCCCCACTCCC
  ATTT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_003725
• Insert Size: 1700 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003725.2, NP_003716.2
• RefSeq Size: 1512 bp
• RefSeq ORF: 954 bp
• Locus ID: 8630
• UniProt ID: O14756
• Cytogenetics: 12q13.3
• Domains: adh_short
• Protein Families: Druggable Genome
• Gene Summary: The protein encoded by this gene has both oxidoreductase and epimerase activities and is involved in androgen catabolism. The oxidoreductase activity can convert 3 alpha-adiol to dihydrotestosterone, while the epimerase activity can convert androsterone to epi-androsterone. Both reactions use NAD+ as the preferred cofactor. This gene is a member of the retinol dehydrogenase family. [provided by RefSeq, Aug 2013]