ATP6V1F (NM_004231) Human Untagged Clone

CAT#: SC117488

ATP6V1F (untagged)-Human ATPase, H+ transporting, lysosomal 14kDa, V1 subunit F (ATP6V1F), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ATP6V1F
• Synonyms: ATP6S14; VATF; Vma7
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_004231 edited
  ATGGCGGGGAGGGGTAAGCTCATCGCAGTGATCGGAGACGAGGACACGGTGACTGGTTTC
  CTGCTGGGCGGCATAGGGGAGCTTAACAAGAACCGCCATCCCAATTTCCTGGTGGTGGAG
  AAGGATACAACCATCAATGAGATCGAAGACACTTTCCGGCAATTTCTAAACCGGGATGAC
  ATTGGCATCATCCTCATCAACCAGTACATCGCAGAGATGGTGCGGCATGCCCTGGACGCC
  CACCAGCAGTCCATCCCCGCTGTCCTGGAGATCCCCTCCAAGGAGCACCCATATGACGCC
  GCCAAGGACTCCATCCTGCGCAGGGCCAGGGGCATGTTCACTGCCGAAGACCTGCGCTAG
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004231 unedited
  CACGAGGGCTTCTGGTGCTCTAGGGTGAGCTCTGCCCGGCTGCAGGGATGGCGGGGAGGG
  GTAAGCTCATCGCAGTGATCGGAGACGAGGACACGGTGACTGGTTTCCTGCTGGGCGGCA
  TAGGGGAGCTTAACAAGAACCGCCATCCCAATTTCCTGGTGGTGGAGAAGGATACAACCA
  TCAATGAGATCGAAGACACTTTCCGGCAATTTCTAAACCGGGATGACATTGGCATCATCC
  TCATCAACCAGTACATCGCAGAGATGGTGCGGCATGCCCTGGACGCCCACCAGCAGTCCA
  TCCCCGCTGTCCTGGAGATCCCCTCCAAGGAGCACCCATATGACGCCGCCAAGGACTCCA
  TCCTGCGCAGGGCCAGGGGCATGTTCACTGCCGAAGACCTGCGCTAGGGGACTCCTCATA
  GCCCTCAGCCCTTCCCTCGTTTCCAGGCCTCTCCCCAGGCTTGCCATCAGCCTTCTTTAC
  TTTTTGAGCCTCTGATTTCCAATTCCCTGCTCCTTCCCACTCCATTAAGAGGCTAGGTGA
  GGCGCTTCTAGGTTGCTGGGGCTCTGCTGGTTAAGGAACAGGAAGCCTGACCATCT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_004231 unedited
  NATGGCACTTCCAGGGCCAGNANAGCACTGGGGAGGGGTCACAGGNATGCCACCCGGGAT
  CTGTTCAGGAAACAGCTATGACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTT
  TTTGGAGAGAAGCAAGAATATGACTTTAATTTAACATCAACAATGACACTGTGTAACAGC
  ACAGGGAAGAGGTAGTGGAGGGAGATGGTCAGGCTTCCTGTTCCTTAACCAGCAGAGCCC
  CAGCAACCTAGAAGCGCCTCACCTAGCCTCTTAATGGAGTGGGAAGGAGCAGGGAATTGG
  AAATCAGAGGCTCAAAAAGTAAAGAAGGCTGATGGCAAGCCTGGGGAGAGGCCTGGAAAC
  GAGGGAAGGGCTGAGGGCTATGAGGAGTCCCCTAGCGCAGGTCTTCGGCAGTGAACATGC
  CCCTGGCCCTGCGCAGGATGGAGTCCTTGGCGGCGTCATATGGGTGCTCCTTGGAGGGGA
  TCTCCAGGACAGCGGGGATGGACTGCTGGTGGGCGTCCAGGGCATGCCGCACCATCTCTG
  CGATGTACTGGTTGATGAGGATGATGCCAATGTCATCCCGGTTTAGAAATTGCCGGAAAG
  TGTCTTCGATCTCATTGATGGTTGTATCCTTCTCCACCACCAGGAAATGGGATGGGCGGT
  TCTTGTTAAGCTCCCCTATGCCGNCCAGCANGAAACCAGNTCACCGTGTCCTCGTCTCCG
  ATCACTGGGGATGAGCTTACCCCTCCCCGCCATTCCTGNAGNCGGGCAGAGCTCACCCTA
  NAGCACAGAAGCCCTCGTGCCGAATCGCCGGCGGCCTATAGGGAGTCCTATACAAAAATT
  TGACGGTTACTAAACAGCTCTGCTATTAGACCTCCACCG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_004231
• Insert Size: 730 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004231.2, NP_004222.2
• RefSeq Size: 691 bp
• RefSeq ORF: 360 bp
• Locus ID: 9296
• UniProt ID: Q16864
• Cytogenetics: 7q32.1
• Domains: ATP-synt_F
• Protein Pathways: Epithelial cell signaling in Helicobacter pylori infection, Metabolic pathways, Oxidative phosphorylation, Vibrio cholerae infection
• Gene Summary: This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c", and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This encoded protein is the V1 domain F subunit protein. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (1) is the predominant transcript.