ARHGAP1 (NM_004308) Human Untagged Clone

CAT#: SC117423

ARHGAP1 (untagged)-Human Rho GTPase activating protein 1 (ARHGAP1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ARHGAP1
• Synonyms: CDC42GAP; p50rhoGAP; RHOGAP; RHOGAP1
• Vector: pCMV6-XL6
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_004308 edited
  GAATTCGGCACGAGGCGCGGTTTCTCTTAAGGTTCTGCAGGGCAAGGCTGTCTGGGACAG
  GCTTGGCCATGGATCCGCTCTCAGAGCTGCAGGATGATCTGACCTTGGATGACACCAGCG
  AGGCTCTGAACCAGCTGAAGCTGGCCTCCATCGATGAGAAGAACTGGCCCTCGGATGAAA
  TGCCTGACTTCCCCAAGTCAGATGACTCCAAAAGCAGCTCCCCGGAACTTGTCACACACC
  TGAAGTGGGATGACCCATACTATGACATCGCCCGGCACCAGATCGTGGAGGTGGCAGGAG
  ATGACAAGTATGGGCGGAAGATCATTGTGTTTAGTGCCTGTCGAATGCCCCCCAGCCACC
  AGCTCGACCACAGCAAGCTCCTGGGGTACCTGAAGCACACCCTGGACCAGTACGTGGAGA
  GTGACTACACACTTCTGTATCTGCACCACGGCCTGACCAGCGACAACAAGCCCTCCCTCA
  GCTGGCTCCGTGATGCCTACCGGGAGTTTGACCGCAAGTACAAGAAGAACATCAAGGCCT
  TGTACATCGTGCATCCAACCATGTTCATCAAAACTCTGCTCATCCTCTTCAAGCCCCTCA
  TCAGCTTCAAGTTCGGGCAGAAGATCTTCTATGTGAATTACCTGAGCGAGCTGAGCGAGC
  ACGTGAAGCTGGAGCAGCTGGGGATCCCTCGCCAAGTGCTCAAATATGACGACTTCCTGA
  AATCCACACAGAAGAGCCCCGCGACAGCCCCCAAGCCCATGCCCCCACGGCCCCCCCTGC
  CCAACCAGCAGTTTGGAGTCTCGCTGCAGCACCTCCAGGAGAAGAATCCAGAGCAGGAGC
  CCATTCCCATTGTACTCAGGGAGACTGTTGCCTACTTACAGGCCCACGCTCTCACCACCG
  AGGGCATCTTCCGGAGGTCGGCCAACACCCAAGTGGTCCGGGAAGTGCAGCAGAAGTACA
  ACATGGGGCTGCCTGTGGATTTCGACCAGTACAATGAGCTGCACCTGCCAGCAGTCATCC
  TCAAGACCTTCCTCCGGGAGCTTCCTGAGCCCCTGCTCACCTTTGACCTCTACCCCCATG
  TGGTGGGCTTCCTCAACATTGATGAAAGCCAGAGGGTGCCAGCGACACTGCAGGTCCTCC
  AGACGCTGCCCGAGGAGAACTACCAGGTGCTTCGTTTCCTGACTGCTTTCCTGGTGCAGA
  TTTCTGCACACAGTGACCAGAACAAGATGACCAACACTAACCTGGCTGTTGTTTTCGGCC
  CTAACCTGCTGTGGGCCAAGGATGCGGCCATCACCCTCAAGGCCATTAATCCCATCAACA
  CCTTCACCAAGTTCCTTCTGGATCACCAAGGGGAGCTGTTCCCAAGCCCGGACCCCAGCG
  GGCTCTGAACCTGGCCCCTGCCCCACCAGCCCCTTCTCTGGTAGCCCGGGTTTGGACTCT
  TCCTCCTGGCATCAGGGGCCATGAAGCCCCCTGGAGAGAGATGTTGGAGCCACCCATCAG
  GCATGCTCTCCCCCACCTCTGTCCAGCCCGCCTCACGGCCCTGGCGGCCTCGCTGTTACC
  AGAAGACGTTTTTCTCTGCCTTACACTTCTCACTGCCTCTCTGGACACCGGGCTTTTGCT
  GGGCTCTCCAGAGCTGGGCTTGGCTCTTCCAGCTGGAGAAGGACTGAGCCCCAGTAACCC
  CTTTCCTGCTTTTTCCTGCCTCTGTTTCCCTAGCAAGTGTGGATGAATCAAATGACTGCC
  GGCTGGGCTGGTAGCAGGGGCCAGTCCTCCCCTCTTGATTGCTGGGAATGAGCAGCTGAG
  CTCTTTGGTGTGGACACCGCCCCAAGCTGAGGTGCCTCCCTATCTGGTAGGCTGCCCTGC
  TTTGCAGAAGCCCCACTGCATGAAGGCCTGAGTGCAAGCACCCTTGCTCCTGTACTGCCC
  CCAGCAGCGCAGTCTGGGGTATTTCCCCTGCTGAGGAGTGACAGGGCTGGAGACTGTGTC
  TGCTTTCTGCCTCCTCTTCCACACATCCTGGGTCAGCCTTTCCCAAGTGATACTGCCCCT
  GGGGTGTCCTAACCCCTTGGTTGTGCAGGGACTGGTAGCTTGGATGACATGGCTCAGAGT
  CCCAGCATCCACTTGAAACCTCCCTCTCCACCTCCCCGCCCCCGAGAGCTACAGCGTGTG
  ATGGAATCAGTGACCGGCCAGGTTAGCCTCTCCCCTCTTGGCCTCCTTTCCCACACGGGC
  AGATCATCAGCCTTCATGTTCTTGTGGACCTGTCTGTTACGGAGTCACACTGGCCCCTGC
  CCCAGCCAGAGGTGACTCAGTGCCTCAGGATCTTATGGCTCCTTCCTCCAGCTGGGGGAC
  TCTTAAAGGGGCTGGGGTAGAGACTAAGAGTGCCCGTAGGAGGGGCAGAGATTGTGCTGA
  TAGGTCAGTCCAGATAGGTGGGTTCTAGCAATTTCCCCAGGGGATTAAGGGGCTCTAGGG
  CCACCTTTTTTTTTTTTTTTCTGAGATGGAGTCTCGCTCTGTCACCAGGCTGGAGTGCAG
  TGGCGCGATCTCGGCACACCTCCGCCTTCTGGGTTCAAGCAATTCTCCTGCCTCAGCCTC
  CTGAGTAGCTGGGACTATAGGTGCATGCCACCATGCCCAGCTAATTTTTGTATTTTTAGT
  AGAGACGGGGTTTCACCATGTTGGCCAGGATGGTCTCGATCTCCTGACCTCATGATCCAC
  CCACCTCGGCCTCCCAAAGTGCTGGGATTCCAGGCGTGAGCCACTGCGCCCGGCCAGCCC
  TGTTGTCTTTCAAAGAACCTCGGCCCCAGTGATGGCTGATCTCACTCCCACTGTCTCGTA
  GAATTACACTCCTTCCTGGGGATCCCTCTTCCTGCAGCCTTGTCCTTGGGGAGTACAGGG
  AAGTACCTGCAGTGACCCTGCCCCCATGACCTTGGCCAGGCAGGATGATTCCAGAGCCCG
  TACTGTGGGGATCCTCACTGACCAGCCCAGCCCCATCCGCAAGCCAGGCTGGGCCTGGGC
  ATAACTTCGCTGGGCTCTCCCAAGACCATCAGCAGTGCATCCAGCCCCCTGGGGCCTGGG
  CTGCTGCTGGTTCTTCATCAGTCCCTTGGTCTCTAGCCTCGGCCAGGGCTGCTCTGCTCT
  CCGCCCTCCTTTGTGTATCAAGTGTCGCTCACAGCCCCATTCACTGGGGAGCCTTTCGGA
  TCTATTTGGTCTTTCTCATGTCCCCCACTGGTCTGTACCCCAGGGAGCGGGTGCTTGTAC
  TGTGTGAATCCAGTGTTCACATTCACACTTAATGACTTCCTTGGCACCAATCATGTATTT
  CACCGTTTGCACTTTTTGTATTTCAATAAAAATGTTGATGCAAAAAAAAAAAAAAAAAAA
  ACTCGAC
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004308 unedited
  ACACCCGCCCGTTGNCGCAAAGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAG
  AGCTCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGGCCGCGAA
  TTCGGCACGAGGCGCGGTTTCTCTTAAGGTTCTGCAGGGCAAGGCTGTCTGGGACAGGCT
  TGGCCATGGATCCGCTCTCAGAGCTGCAGGATGATCTGACCTTGGATGACACCAGCGAGG
  CTCTGAACCAGCTGAAGCTGGCCTCCATCGATGAGAAGAACTGGCCCTCGGATGAAATGC
  CTGACTTCCCCAAGTCAGATGACTCCAAAAGCAGCTCCCCGGAACTTGTCACACACCTGA
  AGTGGGATGACCCATACTATGACATCGCCCGGCACCAGATCGTGGAGGTGGCAGGAGATG
  ACAAGTATGGGCGGAAGATCATTGTGTTTAGTGCCTGTCGAATGCCCCCCAGCCACCAGC
  TCGACCACAGCAAGCTCCTGGGGTACCTGAAGCACACCCTGGACCAGTACGTGGAGAGTG
  ACTACACACTTCTGTATCTGCACCACGGCCTGACCAGCGACAACAAGCCCTCCCTCAGCT
  GGCTCCGTGATGCCTACCGNGAGTTTGACCGCAAGTACAAGAAGAACATCAAGGCCTTGT
  ACATCGTGCATCCAACCATGTTCATCANAACTCTGCTCATCCTCTTCAAGCCCCTCATCA
  GCTTCAAGTTCGGGCAGAAGATCTTCTATGTGAATTACCTGAGCGAGCTGAGCGAGCACG
  TGAAGCTGGAGCAGCTGGGGGATCCCCTCGCCAGTGCTCAATATGACGACTTCTGATCCC
  CCNNNAAAAGCNCCCNNNNNAAGGCCCCCAAGCNCATGCCCCCACGGNCCCCCCTGCCAA
  CCAGAAGAATTGGGAGCTTCGCTGCGCCACCTCCAGGAGAGAATCCGAGCAGGAGCCCT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_004308 unedited
  NNNTTGGATATAGCTATGNNACCGCGGCCGCTTNCTANGATCGAGTTTTTTTTTTTTTTT
  TTTTTGCATCAACATTTTTATTGAAATACAAAAAGTGCAAACGGTGAAATACATGATTGG
  TGCCAAGGAAGTCATTAAGTGTGAATGTGAACACTGGATTCACACAGTACAAGCACCCGC
  TCCCTGGGGTACAGACCAGTGGGGGACATGAGAAAGACCAAATAGATCCGAAAGGCTCCC
  CAGTGAATGGGGCTGTGAGCGACACTTGATACACAAAGGAGGGCGGAGAGCAGAGCAGCC
  CTGGCCGAGGCTAGAGACCAAGGGACTGATGAAGAACCAGCAGCAGCCCAGGCCCCAGGG
  GGCTGGATGCACTGCTGATGGTCTTGGGAGAGCCCAGCGAAGTTATGCCCAGGCCCAGCC
  TGGCTTGCGGATGGGGCTGGGCTGGTCAGTGAGGATCCCCACAGTACGGGCTCTGGAATC
  ATCCTGCCTGGCCAAGGTCATGGGGGCAGGGTCACTGCAGGTACTTCCCTGTACTCCCCA
  AGGACAAGGCTGCAGGAAGAGGGATCCCCAAGAAGGAGTGTAATTCTACGAGACAGTGGG
  AGTGAGATCAGCCATCACTGGGGCCGAAGTTCTTTGAAAGAAACAAGGCTGGCCGGGCGC
  AATGGCTCACGCCTGGGATTCCAGCACTTTGGGAGGCCCAAGTGGGTGGATCATGAGGTC
  AGGAGATCGAGACCCTCCTGGCCAACATGTGAAAACCCCTCTTTACTAAAAAACAAAATT
  AACTGGGCTGGGGGGCATGCCCCTTTTGTCCCCGCTTCTTAGGGAGGGCGAAGGCAGGAG
  AATTGCTTGAACCCCCAAAGCCGGGAGTGGGTCCCAGAT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_004308
• Insert Size: 3600 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004308.2, NP_004299.1
• RefSeq Size: 3372 bp
• RefSeq ORF: 1320 bp
• Locus ID: 392
• UniProt ID: Q07960
• Cytogenetics: 11p11.2
• Domains: RhoGAP, SEC14
• Gene Summary: This gene encodes a member of a large family of proteins that activate Rho-type guanosine triphosphate (GTP) metabolizing enzymes. The encoded protein contains a SRC homology 3 domain and interacts with Bcl-2-associated protein family members. [provided by RefSeq, Aug 2012]