PABP2 (PABPN1) (NM_004643) Human Untagged Clone

CAT#: SC117245

PABPN1 (untagged)-Human poly(A) binding protein, nuclear 1 (PABPN1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PABP2
• Synonyms: OPMD; PAB2; PABII; PABP-2; PABP2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_004643 edited
  GAATTCGGCACGAGGGTTCTAGAGAGGGTAGCTTTTCTTTTATCACGACCCTCGCATGGG
  GCGAGGGAAATGGCCGAGCATGGCTGAGGCCGCGCTCTGGCCGAGAGCAGGGCACAGCCC
  CTGCGTTGGTTCCTCTTAAGCTGTCCTCCATACCCTCCCCACTTATATTAGGAGCTGGAA
  GCTATCAAAGCTCGAGTCAGGGAGATGGAGGAAGAAGCTGAGAAGCTAAAGGAGCTACAG
  AACGAGGTAGAGAAGCAGATGAATATGAGTCCACCTCCAGGCAATGCTGGCCCAGTGATC
  ATGTCCATTGAGGAGAAGATGGAGGCTGATGCCCGTTCCATCTATGTTGGCAATGTGGAC
  TATGGTGCAACAGCAGAAGAGCTGGAAGCTCACTTTCATGGCTGTGGTTCAGTCAACCGT
  GTTACCATACTGTGTGACAAATTTAGTGGCCATCCCAAAGGGTTTGCGTATATAGAGTTC
  TCAGACAAAGAGTCAGTGAGGACTTCCTTGGCCTTAGATGAGTCCCTATTTAGAGGAAGG
  CAAATCAAGGTGATCCCAAAACGAACCAACAGACCAGGCATCAGCACAACAGACCGGGGT
  TTTCCACGAGCCCGCTACCGCGCCCGGACCACCAACTACAACAGCTCCCGCTCTCGATTC
  TACAGTGGTTTTAACAGCAGGCCCCGGGGTCGCGTCTACAGGGGCCGGGCTAGAGCGACA
  TCATGGTATTCCCCTTACTAAAAAAAGTGTGTATTAGGAGGAGAGAGAGGAAAAAAAGAG
  GAAAGAAGGAAAAAAAAAAGAATTAAAAAAAAAAAAAGAAAAACAGAAGATGACCTTGAT
  GGAAAAAAAATATTTTTTAAAAAAAAGATATACTGTGGAAGGGGGGAGAATCCCATAACT
  AACTGCTGAGGAGGGACCTGCTTTGGGGAGTAGGGGAAGGCCCAGGGAGTGGGGCAGGGG
  GCTGCTTATTCACTCTGGGGATTCGCCATGGACACGTCTCAACTGCGCAAGCTGCTGCCC
  ATGTTTCCCTGCCCCACTTCACCCCCTTGGGGGCTGCTCAAGGGTAGGTGGGCGTGGGTG
  GTAGGAGGTTTTTTTTTTACCCAGGGCTCTGGAAGGACACCAAACTGTTCTGCTTGTTAC
  CTTCCCTCCGTCTTCTCCTCGCCTTTCACAGTCCCCTCCTGCCTGCTCCTGTCCAGCCAG
  GTCTACCACCCACCCCACCCCTCTTCTCCGGCTCCCTGCCCCTCCAGATTGCCTGGTGAT
  CTATTTTGTTTCCTTTTGTGTTTCTTTTTCTGTTTTGAGTGTCTTTCTTTGCAGGTTTCT
  GTAGCCGGAAGATCTCCGTTCCGCTCCCAGCGGCTCCAGTGTAAATTCCCCTTCCCCCTG
  GGGAAATGCACTACCTTGTTTTGGGGGGTTTAGGGGTGTTTTTGTTTTTCAGTTGTTTTG
  TTTTTTTTTTTTCCTTTGCCTTTTTTCCCTTTTATTTGGAGGGAATGGGAGGAAGTGGGA
  ACAGGGAGGTGGGAGGTGGATTTTGTTTATTTTTTTAGCTCATTTCCAGGGGTGGGAATT
  TTTTTTTAATATGTGTCATGAATAAAGTTGTTTTGAAXXXXXXXXXXXXXAAAAAAAAAA
  AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
  AAAAAAAAAAAAAAAAAAACTCGAC
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004643 unedited
  NGGGTCAAATTTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGGTTCTAGA
  GAGGGTAGCTTTTCTTTTATCACGACCCTCGCATGGGGCGAGGGAAATGGCCGAGCATGG
  CTGAGGCCGCGCTCTGGCCGAGAGCAGGGCACAGCCCCTGCGTTGGTTCCTCTTAAGCTG
  TCCTCCATACCCTCCCCACTTATATTAGGAGCTGGAAGCTATCAAAGCTCGAGTCAGGGA
  GATGGAGGAAGAAGCTGAGAAGCTAAAGGAGCTACAGAACGAGGTAGAGAAGCAGATGAA
  TATGAGTCCACCTCCAGGCAATGCTGGCCCAGTGATCATGTCCATTGAGGAGAAGATGGA
  GGCTGATGCCCGTTCCATCTATGTTGGCAATGTGGACTATGGTGCAACAGCAGAAGAGCT
  GGAAGCTCACTTTCATGGCTGTGGTTCAGTCAACCGTGTTACCATACTGTGTGACAAATT
  TAGTGGCCATCCCAAAGGGTTTGCGTATATAGAGTTCTCAGACAAAGAGTCAGTGAGGAC
  TTCCTTGGCCTTAGATGAGTCCCTATTTAGAGGAAGGCAAATCAAGGTGATCCCAAAACG
  AACCAACAGACCAGGCATCAGCACAACAGACCGGGGTTTTCCACGAGCCCGCTACCGCGC
  CCGGACCACCAACTACAACAGCTCCCGCTCTCGATTCTACAGTGGTTTTAACAGCAGGCC
  CCGGNGTCGCGTCTACAGGGGCCGGGCTAGAGCGACATCATGGTATTCCCCTTACAAAAA
  AAGTGTGTATTANGAGGAGAGAGAGGGAAAAAAGAGGAAAGAAGGAAAAAAAAAAGAATT
  AAAAAAAAAAAAGAAAACCCGAGATGACCTTGATGGAAAAAAATATTTTTAAAAAAGATT
  ACTGTGGAAGGGGGGAGATCCCATACTACTGCTGAGGAG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_004643 unedited
  ATCCGTAGCCAGGAAAATCGTAAATACAACTTNTATNATGNACACATATTAAAAAAAAAT
  CCCACCCCTGGAAATGAGCTNAAAAAATAAACAAAATCCACCTCCCACCTCCCTGTTCCC
  ACTTCCTCCCATTCCCTCCAAATAAAAGGGAAAAAAGGCATCGGCAAAAAAAAAAAACAA
  AACAACTGAAAAACAAAAACACCCCTAAACCCCCCAAAACAAGGTAGTGCATTTCCCCAG
  GGGGAAGGGGAATTTACACTGGACCCGCTGGGAGCGGAACGGATACCTTTCCGGCTCCAG
  AACCCTGCTTTGAAAGACTCTCCTTCCTGAAAATGAATCACTCCCCGCACCCTAATATAC
  CACCACCCTATCTCCCCGCGCATCGCCTCCGACACACAGCGACGACTGCCTATCCCCCCC
  TCCTCCTCCCCTCCCTGCTCTCAGTCACTGTCATCTTCCCTCCCCAACAACTCCTGCCTC
  GCCCCTGCCCATATCCCCGCCTCCCCCTTCCCCCCCTCCTTTCCCCCCTACCTCCATCCT
  CCCCTCCCTCTCCCCCCCTTCCCATTCTCTTCCCTCGCCATCGACCTCCTTCTTACCTCT
  ACCCAACTCTTCTTTTCTTCATCTTCTTTTCCTCCTCTCTCCCCCCTTCCCCCCTTCCCC
  CTCCCCTCTTTATTTTACTTTCCCACACACCCCCCTATCCTTCGCCCGCTCCCCACCCCT
  CTTTTCCCTCTCCCCCCCCTACCCCCTCTCCATTTCTTCCGCTTTGTCTCTTTATCTCCG
  ACTGTCCTATTCTCCCCCTCCCCCCTCCTTTTTCACCCCCTACGTTACCCCACTACCTAT
  ATACTACACACAACTTTATATCTCTCTCTCATGTTATTCCTGTCCGCGCGATATTAATAG
  ATATTATACCTATCCAGTCCTCCGTCATTCACTCCCTCATATCCTCATATATAAAAAACT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_004643
• Insert Size: 1750 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.

Reference Data

• RefSeq: NM_004643.1, NP_004634.1
• RefSeq Size: 3107 bp
• RefSeq ORF: 921 bp
• Locus ID: 8106
• UniProt ID: Q86U42
• Cytogenetics: 14q11.2
• Domains: RRM
• Protein Families: Druggable Genome
• Gene Summary: This gene encodes an abundant nuclear protein that binds with high affinity to nascent poly(A) tails. The protein is required for progressive and efficient polymerization of poly(A) tails at the 3' ends of eukaryotic transcripts and controls the size of the poly(A) tail to about 250 nt. At steady-state, this protein is localized in the nucleus whereas a different poly(A) binding protein is localized in the cytoplasm. This gene contains a GCG trinucleotide repeat at the 5' end of the coding region, and expansion of this repeat from the normal 6 copies to 8-13 copies leads to autosomal dominant oculopharyngeal muscular dystrophy (OPMD) disease. Related pseudogenes have been identified on chromosomes 19 and X. Read-through transcription also exists between this gene and the neighboring upstream BCL2-like 2 (BCL2L2) gene. [provided by RefSeq, Dec 2010]