LOXL1 (NM_005576) Human Untagged Clone

CAT#: SC116666

LOXL1 (untagged)-Human lysyl oxidase-like 1 (LOXL1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: LOXL1
• Synonyms: LOL; LOXL
• Vector: pCMV6-XL6
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC116666 sequence for NM_005576 edited (data generated by NextGen Sequencing)
  ATGGCTCTGGCCCGAGGCAGCCGGCAGCTGGGGGCCCTGGTGTGGGGCGCCTGCCTGTGC
  GTGCTGGTGCACGGGCAGCAGGCGCAGCCCGGGCAGGGCTCGGACCCCGCCCGCTGGCGG
  CAGCTGATCCAGTGGGAGAACAACGGGCAGGTGTACAGCTTGCTCAACTCGGGCTCAGAG
  TACGTGCCGGCCGGACCTCAGCGCTCCGAGAGTAGCTCCCGGGTGCTGCTGGCCGGCGCG
  CCCCAGGCCCAGCAGCGGCGCAGCCACGGGAGCCCCCGGCGTCGGCAGGCGCCGTCCCTG
  CCCCTGCCGGGGCGCGTGGGCTCGGACACCGTGCGCGGCCAGGCGCGGCACCCATTCGGC
  TTTGGCCAGGTGCCCGACAACTGGCGCGAGGTGGCCGTCGGGGACAGCACGGGCATGGCC
  CGGGCCCGCACCTCCGTCTCCCAGCAACGGCACGGGGGCTCCGCCTCCTCGGTCTCGGCT
  TCGGCCTTCGCCAGCACCTACCGCCAGCAGCCCTCCTACCCGCAGCAGTTCCCCTACCCG
  CAGGCGCCCTTCGTCAGCCAGTACGAGAACTACGACCCCGCGTCGCGGACCTACGACCAG
  GGTTTCGTGTACTACCGGCCCGCGGGCGGCGGCGTGGGCGCGGGGGCGGCGGCCGTGGCC
  TCGGCGGGGGTCATCTACCCCTACCAGCCCCGGGCGCGCTACGAGGAGTACGGCGGCGGC
  GAAGAGCTGCCCGAGTACCCGCCTCAGGGCTTCTACCCGGCCCCCGAGAGGCCCTACGTG
  CCGCCGCCGCCGCCGCCCCCCGACGGCCTGGACCGCCGCTACTCGCACAGTCTGTACAGC
  GAGGGCACCCCCGGCTTCGAGCAGGCCTACCCTGACCCCGGTCCCGAGGCGGCGCAGGCC
  CATGGCGGAGACCCACGCCTGGGCTGGTACCCGCCCTACGCCAACCCGCCGCCCGAGGCG
  TACGGGCCGCCGCGCGCGCTGGAGCCGCCCTACCTGCCGGTGCGCAGCTCCGACACGCCC
  CCGCCGGGTGGGGAGCGGAACGGCGCGCAGCAGGGCCGCCTCAGCGTGGGCAGCGTGTAC
  CGGCCCAACCAGAACGGCCGCGGTCTCCCTGACTTGGTCCCAGACCCCAACTATGTGCAA
  GCATCCACTTATGTGCAGAGAGCCCACCTGTACTCCCTGCGCTGTGCTGCGGAGGAGAAG
  TGTCTGGCCAGCACAGCCTATGCCCCTGAGGCCACCGACTACGATGTGCGGGTGCTACTG
  CGCTTCCCCCAGCGCGTGAAGAACCAGGGCACAGCAGACTTCCTCCCCAACCGGCCACGG
  CACACCTGGGAGTGGCACAGCTGCCACCAGCATTACCACAGCATGGACGAGTTCAGCCAC
  TACGACCTACTGGATGCAGCCACAGGCAAGAAGGTGGCCGAGGGCCACAAGGCCAGTTTC
  TGCCTGGAGGACAGCACCTGTGACTTCGGCAACCTCAAGCGCTATGCATGCACCTCTCAT
  ACCCAGGGCCTGAGCCCAGGCTGCTATGACACCTACAATGCGGACATCGACTGCCAGTGG
  ATCGACATAACCGACGTGCAGCCTGGGAACTACATCCTCAAGGTGCACGTGAACCCAAAG
  TATATTGTTTTGGAGTCTGACTTCACCAACAACGTGGTGAGATGCAACATTCACTACACA
  GGTCGCTACGTTTCTGCAACAAACTGCAAAATTGTCCAATCCTGA
  
  Clone variation with respect to NM_005576.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005576 unedited
  GCACGAGGGAAAGCGCCAGCCGAGCGGCCAGCCAGTGCGGGGCTGGCCATGTAAGGCCCA
  CAGGCGGTCCTGCCCGCCCGGTGCCCTGCGGAGAGCCTCGTGCAGCCCTGGGCACCGCCC
  CTGCCCTGCCCTGACCCCTTGGCCTTGAAATGCTGTCATCGGAGGAGCCGTCCCGCTCGG
  GACAAGGCCAGCATGGACAAAGCTAGAGCTGGGGCAAGCAAGGAGCCTTCCTGTCCTCGA
  GGCCGTGGGAAGAGAAGCACGCCCAGGGGGCCACTCCTGAGAGCCTCTCTGTCCACCAGG
  CCTCTGCAGAGGGGTCACCATGGCTCTGGCCCGAGGCAGCCGGCAGCTGGGGGCCCTGGT
  GTGGGGCGCCTGCCTGTGCGTGCTGGTGCACGGGCAGCAGGCGCAGCCCGGGCAGGGCTC
  GGACCCCGCCCGCTGGCGGCAGCTGATCCAGTGGGAGAACAACGGGCAGGTGTACAGCTT
  GCTCAACTCGGGCTCAGAGTACGTGCCGGCCGGACCTCAGCGCTCCGAGAGTAGCTCCCG
  GGTGCTGCTGGCCGGCGCGCCCCAGGCCCAGCAGCGGCGCAGCCACGGGAGCCCCCGGCG
  TCGGCAGGCGCCGTCCCTGCCCCTGCCGGNGCGCGTGGGCTCGGACACCGT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_005576 unedited
  ACTTATGTACGCGGCCGCAATTTANGATCGATTTTTTTTCTTTTTTTNTTTTTAGAAAAA
  ACCTTGTTTTATTCAGCCCANACCTGGGCAATCTAGCTCTGTGGTATGGCCAAAGTATAA
  AAAATAAAAACCAACAACAACAAAACAACACTGTAGAGAAAAGTGAAGTATAAAATGGGG
  TCTGGCGTCCGGAATCCCTGTGGTTTTCATCCACGTTCGCTCCCTGAGGCCGGCAGGGAG
  GGATGCCCCTCCCTGCCTGTGGGTTGGGGGCTGGGCCCCTCGGCGGGAGGCTGCCCGGGG
  AGCAAGGCCTGCTTTGGAAGGGGAGAGATTGGCCATCTGTCCCTCCCGGAGATCAGGATT
  GGACAATTTTGCAGTTTGTTGCAGAAACGTAGCGACCTGTGTAGTGAATGTTGCATCTCA
  CCACGTTGTTGGTGAAGTCAGACTCCAAAACAATATACTTTGGGTTCACGTGCACCTTGA
  GGATGTAGTTCCCAGGCTGCACGTCGGTTATGTCGATCCACTGGCAGTCGATGTCCGCAT
  TGTAAGTGTCATAGCAGCCTGAGCTCAAGCCCTGGGTATGAGAGGTGCATGCATAGCGCT
  TGAGGTTGCCGAAGTCACAGGTGCTGTCCTCCAGGCAGAAACTGGCCTTGTGGCCCTCGG
  CCACCTTCTTGCCTGTGGCTGCATCCAGTAGGTCGTAATGGCTGAACTCGTCCATGCTGT
  GGTAATGCTGGTGGCAGCTGTGCCACTCCCAGGTGTGCCGTGGCCCGTTGGGGAGGAAGT
  CTGCTGTGCCCTGGTTCTCACGCGCTGGGGGAACGCANTAACACCCGACATCGTATTCCG
  TGGGCTCAGGGCATAAGCTGGGCTGGCANACACTTTTCCTCCGCACACAAGCAGGAGTTC
  AAGTGGG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_005576
• Insert Size: 2500 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005576.2, NP_005567.2
• RefSeq Size: 2361 bp
• RefSeq ORF: 1725 bp
• Locus ID: 4016
• UniProt ID: Q08397
• Cytogenetics: 15q24.1
• Domains: Lysyl_oxidase
• Protein Families: Secreted Protein
• Gene Summary: This gene encodes a member of the lysyl oxidase family of proteins. The prototypic member of the family is essential to the biogenesis of connective tissue, encoding an extracellular copper-dependent amine oxidase that catalyzes the first step in the formation of crosslinks in collagen and elastin. The encoded preproprotein is proteolytically processed to generate the mature enzyme. A highly conserved amino acid sequence at the C-terminus end appears to be sufficient for amine oxidase activity, suggesting that each family member may retain this function. The N-terminus is poorly conserved and may impart additional roles in developmental regulation, senescence, tumor suppression, cell growth control, and chemotaxis to each member of the family. Mutations in this gene are associated with exfoliation syndrome. [provided by RefSeq, Jan 2016]