ACTR1A (NM_005736) Human Untagged Clone

CAT#: SC116595

ACTR1A (untagged)-Human ARP1 actin-related protein 1 homolog A, centractin alpha (yeast) (ACTR1A)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ACTR1A
• Synonyms: ARP1; Arp1A; CTRN1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC116595 sequence for NM_005736 edited (data generated by NextGen Sequencing)
  ATGGAGTCCTACGATGTGATCGCCAACCAGCCTGTCGTGATCGACAACGGATCCGGTGTG
  ATTAAAGCTGGTTTTGCTGGTGATCAGATCCCCAAATACTGCTTTCCAAACTATGTGGGC
  CGACCCAAGCACGTTCGTGTCATGGCAGGAGCCCTTGAAGGCGACATCTTCATTGGCCCC
  AAAGCTGAGGAGCACCGAGGGCTGCTTTCAATCCGCTATCCCATGGAGCATGGCATCGTC
  AAGGATTGGAACGACATGGAACGCATTTGGCAATATGTCTATTCTAAGGACCAGCTGCAG
  ACTTTCTCAGAGGAGCATCCTGTGCTCCTGACTGAGGCGCCTTTAAACCCACGAAAAAAC
  CGGGAACGAGCTGCCGAAGTTTTCTTCGAGACCTTCAATGTGCCCGCTCTTTTCATCTCC
  ATGCAAGCTGTACTCAGCCTTTACGCTACAGGCAGGACCACAGGGGTGGTGCTGGATTCT
  GGGGATGGAGTCACCCATGCTGTGCCCATCTATGAGGGCTTTGCCATGCCCCACTCCATC
  ATGCGCATCGACATCGCGGGCCGGGACGTCTCTCGCTTCCTGCGCCTCTACCTGCGTAAG
  GAGGGCTACGACTTCCACTCATCCTCTGAGTTTGAGATTGTCAAGGCCATAAAAGAAAGA
  GCCTGTTACCTATCCATAAACCCCCAAAAGGATGAGACGCTAGAGACAGAGAAAGCTCAG
  TACTACCTGCCTGATGGCAGCACCATTGAGATTGGTCCTTCCCGATTCCGGGCCCCTGAG
  TTGCTCTTCAGGCCAGATTTGATTGGAGAGGAGAGTGAAGGCATCCACGAGGTCCTGGTG
  TTCGCCATTCAGAAGTCAGACATGGACCTGCGGCGCACGCTTTTCTCTAACATTGTCCTC
  TCAGGAGGCTCTACCCTGTTCAAAGGTTTTGGTGACAGGCTCCTGAGTGAAGTGAAGAAA
  CTAGCTCCAAAAGATGTGAAGATCAGGATATCTGCACCTCAGGAGAGACTGTATTCCACG
  TGGATTGGGGGCTCCATCCTTGCCTCCCTGGACACCTTTAAGAAGATGTGGGTCTCCAAA
  AAGGAATATGAGGAAGACGGTGCCCGATCCATCCACAGAAAAACCTTCTAA
  
  Clone variation with respect to NM_005736.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005736 unedited
  NGTTCACATTTGTATACGACTCCTATAGGCGGCCCGCGAATTCGCACGAGGGGCGGACCC
  GGCTGGGCAGTTCCTTCCCCANAAGGAGAGATTCCTCTGCCATGGAGTCCTACGATGTGA
  TCGCCAACCAGCCTGTCGTGATCGACAACGGATCCGGTGTGATTAAAGCTGGTTTTGCTG
  GTGATCAGATCCCCAAATACTGCTTTCCAAACTATGTGGGCCGACCCAAGCACGTTCGTG
  TCATGGCAGGAGCCCTTGAAGGCGACATCTTCATTGGCCCCAAAGCTGAGGAGCACCGAG
  GGCTGCTTTCAATCCGCTATCCCATGGAGCATGGCATCGTCAAGGATTGGAACGACATGG
  AACGCATTTGGCAATATGTCTATTCTAAGGACCAGCTGCAGACTTTCTCAGAGGAGCATC
  CTGTGCTCCTGACTGAGGCGCCTTTAAACCCACGAAAAAACCGGGAACGAGCTGCCGAAG
  TTTTCTTCGAGACCTTCAATGTGCCCGCTCTTTTCATCTCCATGCAAGCTGTACTCAGCC
  TTTACGCTACAGGCAGGACCACAGGGGTGGTGCTGGATTCTGGGGATGGAGTCACCCATG
  CTGTGCCCATCTATGAGGGCTTTGCCATGCCCCACTCCATCATGCGCATCGACATCGCGG
  GCCGGGACGTCTCTCGCTTCCTGCGCCTCTACCTGCGTAAGGAGGGCTACGACTTCCACT
  CATCCTCTGAGTTTGAGATTGTCAAGGCCATAAAAGAAAGAGCCTGTTACCTATCCATAA
  ACCCCCAAAGGATGAGACGCTAGAGACAGAGAAAGCTCAGTACTACCTGCCTNGATGCAG
  CACCATTGAGATTGGTCCTTTTCCGATTNCGGNNCCCTGAGNTGCTCTTCAGCCAGATTT
  GATTGGAGAG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_005736 unedited
  TGGACCGGCGGCCGCAATCTAGNATCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT
  TTTTTTTTTTTTTTTTGAGGTTTAAAGGGGAGTTAACTTCAAAAAAAGGGGAAAATGATG
  TCCCCACCTTAAAAGGGTTTTTTGGGGATGGATCGGGCACCGGCTTCCTCATATTCCTTT
  TTGGAAACCCACATTTTTTTAAAGGGGTCCAGGGAGGCAAGGGTGGAGCCCCCAATCCAC
  GGGGAATACAATCTCTCCTGAGGGGCAAATATCCTGATCTTCACATTTTTTGGAGCTAGT
  TTCTTCACTTCACTCAAGAGCCTGTCACCAAAACCTTTGAACAGGGTAAAACCTCCTGAA
  AGGACAATGTTAAAAAAAAACGTGCGCCCCAAGGCCCTGTCTGACTTCTGAATGGGGAAC
  ACCAAGACCCCGGGGATGCCTTCACTCTCCTTTCCAAACAAATTTGGCCTGAAAAACAAC
  TCAGGGGCCCGGAATCGGGAAGGACCAATCTCAATGGGGCTGCCATCAGGCAGGGAGTAC
  TGAGCTTTCTCTGTCTCTAACGTCTCATCCTTTTGGGGGTTTATGGATAGGTAACAAGCT
  CTTTCTTTTATGGCCTTGACAATCTCAAACTCAAAGGATGAGTGGAAATCGTACCCCTCC
  TTACCCAGGTAGAGGCGCAAGAAACGAGAGACCTCCCCGGCCCCCGAATGTCAATGCCCA
  TGATGGAATGGGGGCATGGCAAAACCCCTCATAGAGGGGCCCAGATTGGGTGACTTCCAT
  CCCANAATCCACAACCACCCCTGGGGGCCCTCCCTGTACCGAAAAGGCTGATTCCAACTT
  TCCTTGAAAATGAAAAAGACGGCCCATCTGAAGGTTCAAAAAAACTTCGCACGCTCGTCC
  CGCGTTTTTTCGGGGTTAAAGGGCCCTCATCCAGGACCA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_005736
• Insert Size: 1300 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005736.2, NP_005727.1
• RefSeq Size: 2828 bp
• RefSeq ORF: 1131 bp
• Locus ID: 10121
• UniProt ID: P61163
• Cytogenetics: 10q24.32
• Domains: ACTIN
• Gene Summary: This gene encodes a 42.6 kD subunit of dynactin, a macromolecular complex consisting of 10-11 subunits ranging in size from 22 to 150 kD. Dynactin binds to both microtubules and cytoplasmic dynein. It is involved in a diverse array of cellular functions, including ER-to-Golgi transport, the centripetal movement of lysosomes and endosomes, spindle formation, chromosome movement, nuclear positioning, and axonogenesis. This subunit is present in 8-13 copies per dynactin molecule, and is the most abundant molecule in the dynactin complex. It is an actin-related protein, and is approximately 60% identical at the amino acid level to conventional actin. [provided by RefSeq, Jul 2008]